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Controllable synthesis and stabilization of Tamarix aphylla-mediated copper oxide nanoparticles for the management of Fusarium wilt on musk melon

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Abstract

Excessive use of pesticides and mineral fertilizers poses a serious threat to ecoenvironment sustainability and human health. Nano pesticides or Nano fungicides have attained great attention in the field of agriculture due to their unique characteristics, by improving crop growth with enhancing pathogenesis-related defense system. However, there is a need to develop a sustainable mechanism for the synthesis of fungicides which replace the chemical pesticides to avoid their hazardous impact. Here in, Tamarix aphylla mediated CuO-Nanoparticles (NPs) were synthesized, characterized and their activity was evaluated under in-vitro and in-vivo conditions. The structural and elemental analysis of NPs were carried out by using X-ray powder diffraction (XRD), Fourier transformed infrared spectroscopy (FTIR), UV–visible spectrophotometer, Scanning electron microscope (SEM) and Transmission electron microscope (TEM). In the greenhouse, at an optimum concentration of 50 mg/L reduced disease severity very effectively and enhanced plant growth. Application of NPs also assisted in the induction of systemic response of defense-related genes in melon. Under In vitro condition at 100 mg/L significantly reduced mycelial growth (84.5%) by directly acting on the pathogenic cell wall. Our work confirmed that dosedependent concentration of T. aphylla extract based biological CuO-NPs enhance plant growth and help to effectively resist against F. oxysporum infection.

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References

  • Acharyulu N, Dubey R, Swaminadham V, Kalyani R, Kollu P, Pammi S (2014) Green synthesis of CuO nanoparticles using Phyllanthus amarus leaf extract and theirantibacterial activity against multidrug resistance bacteria. Int J Eng Res Technol 3(4)

  • Adisa IO, Pullagurala VLR, Peralta-Videa JR, Dimkpa CO, Elmer WH, Gardea-Torresdey JL, White JC (2019) "Recent advances in nano-enabled fertilizers and pesticides: a critical review of mechanisms of action. Environ Sci Nano 6(7):2002–2030

    Article  CAS  Google Scholar 

  • Alam T, Khan RAA, Ali A, Sher H, Ullah Z, Ali M (2019) Biogenic synthesis of iron oxide nanoparticles via Skimmia laureola and their antibacterial efficacy against bacterial wilt pathogen Ralstonia solanacearum. Mater Sci Eng C 98:101–108

    Article  CAS  Google Scholar 

  • Alengebawy A, Abdelkhalek ST, Qureshi SR, Wang M-Q (2021) Heavy metals and pesticides toxicity in agricultural soil and plants: ecological risks and human health implications. Toxics 9(3):42

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Alghuthaymi MA, Almoammar H, Rai M, Said-Galiev E, Abd-Elsalam KA (2015) Myconanoparticles: synthesis and their role in phytopathogens management. Int J Biol Macromol 29(2):221–236

    CAS  Google Scholar 

  • Asghar MA, Zahir E, Shahid SM, Khan MN, Asghar MA, Iqbal J, Walker G (2018) Iron, copper and silver nanoparticles: green synthesis using green and black tea leaves extracts and evaluation of antibacterial, antifungal and aflatoxin B1 adsorption activity. LWT 90:98–107

    Article  CAS  Google Scholar 

  • Behravan M, Panahi AH, Naghizadeh A, Ziaee M, Mahdavi R, Mirzapour A (2019) Facile green synthesis of silver nanoparticles using Berberis vulgaris leaf and root aqueous extract and its antibacterial activity. Int J Biol Macromo 124:148–154

    Article  CAS  Google Scholar 

  • Bibi S, Afzal M, Aziz N, Din B, Khan M, Khan A, Komal H (2015) Antifungal activity of Tamarix aphylla (L.) Karst. stem-bark extract against some pathogenic fungi. Am Eurasian J Sustain Agric 15:541–545

    Google Scholar 

  • Borgatta J, Ma C, Hudson-Smith N, Elmer W, Plaza Pérez CD, De La Torre-Roche R, Zuverza-Mena N, Haynes CL, White JC, Hamers RJ (2018) Copper based nanomaterials suppress root fungal disease in watermelon (Citrullus lanatus): role of particle morphology, composition and dissolution behavior. ACS Sustain Chem Eng 6(11):14847–14856

    Article  CAS  Google Scholar 

  • Çalışkan G, Mutaf T, Öncel SS, Elibol M (2019) Green synthesis of metal nanoparticles using Microalga galdieria sp. In: pro ICMBE

  • Carmona-Hernandez S, Reyes-Pérez JJ, Chiquito-Contreras RG, Rincon-Enriquez G, Cerdan-Cabrera CR, Hernandez-Montiel LG (2019) Biocontrol of postharvest fruit fungal diseases by bacterial antagonists: a review. Agronomy 9(3):121

    Article  CAS  Google Scholar 

  • Castillo-Henríquez L, Alfaro-Aguilar K, Ugalde-Álvarez J, Vega-Fernández L, de Oca-Vásquez GM, Vega-Baudrit JR (2020) Green synthesis of metal nanoparticles from plant extracts, and their possible application as antimicrobial agents in the agricultural area

  • Chmielowska J, Veloso J, Gutierrez J, Silvar C, Díaz J (2010) Cross-protection of pepper plants stressed by copper against a vascular pathogen is accompanied by the induction of a defence response. Plant Sci 178(2):176–182

    Article  CAS  Google Scholar 

  • Chomicki G, Schaefer H, Renner SS (2020) Origin and domestication of Cucurbitaceae crops: insights from phylogenies, genomics and archaeology. New Phytol 226(5):1240–1255

    Article  PubMed  Google Scholar 

  • Choudhary RC, Kumaraswamy R, Kumari S, Sharma S, Pal A, Raliya R, Biswas P, Saharan V (2017) Cu-chitosan nanoparticle boost defense responses and plant growth in maize (Zea mays L). Sci Rep 7(1):1–11

    Article  CAS  Google Scholar 

  • Chun S-C, Chandrasekaran M (2019) Chitosan and chitosan nanoparticles induced expression of pathogenesis-related proteins genes enhances biotic stress tolerance in tomato. Int J Biol Macromol 125:948–954

    Article  CAS  PubMed  Google Scholar 

  • Devi HS, Boda MA, Shah MA, Parveen S, Wani AH (2019) Green synthesis of iron oxide nanoparticles using Platanus orientalis leaf extract for antifungal activity. Green Process Synth 8(1):38–45

    Article  CAS  Google Scholar 

  • Dukare AS, Paul S, Nambi VE, Gupta RK, Singh R, Sharma K, Vishwakarma RK (2019) Exploitation of microbial antagonists for the control of postharvest diseases of fruits: a review. Crit Rev Food Sci Nutr 59(9):1498–1513

    Article  CAS  PubMed  Google Scholar 

  • Elmer W, White JC (2018) The future of nanotechnology in plant pathology. Annu Rev Phytopathol 56:111–133

    Article  CAS  PubMed  Google Scholar 

  • Everts KL, Himmelstein JC (2015) Fusarium wilt of watermelon: towards sustainable management of a re-emerging plant disease. Crop Prot 73:93–99

    Article  Google Scholar 

  • Fisher MC, Henk DA, Briggs CJ, Brownstein JS, Madoff LC, McCraw SL, Gurr SJ (2012) Emerging fungal threats to animal, plant and ecosystem health. Nature 484(7393):186–194

    Article  CAS  PubMed  Google Scholar 

  • Fones HN, Bebber DP, Chaloner TM, Kay WT, Steinberg G, Gurr SJ (2020) Threats to global food security from emerging fungal and oomycete crop pathogens. Nat Food 1(6):332–342

    Article  Google Scholar 

  • Gao L-W, Yang S-L, Wei S-W, Huang D-F, Zhang Y-D (2020) "Supportive role of the Na+ transporter CmHKT1; 1 from Cucumis melo in transgenic Arabidopsis salt tolerance through improved K+/Na+ balance. Plant Mol Biol 103:561–580

    Article  CAS  PubMed  Google Scholar 

  • Hemmati S, Baghayeri M, Kazemi S, Veisi H (2018) Biosynthesis of silver nanoparticles using oak leaf extract and their application for electrochemical sensing of hydrogen peroxide. Appl Organomet Chem 32(11):e4537

    Article  CAS  Google Scholar 

  • Hirpara DG, Gajera H (2020) Green synthesis and antifungal mechanism of silver nanoparticles derived from chitin-induced exometabolites of Trichoderma interfusant. Appl Organomet Chem 34(3):e5407

    Article  CAS  Google Scholar 

  • Ijaz I, Gilani E, Nazir A, Bukhari A (2020) Detail review on chemical, physical and green synthesis, classification, characterizations and applications of nanoparticles. Green Chem Lett Rev 13(3):59–81

    Article  CAS  Google Scholar 

  • Iravani S, Korbekandi H, Mirmohammadi SV, Zolfaghari B (2014) Synthesis of silver nanoparticles: chemical, physical and biological methods. Res Pharm Sci 9(6):385

    CAS  PubMed  PubMed Central  Google Scholar 

  • Jadhav MS, Kulkarni S, Raikar P, Barretto DA, Vootla SK, Raikar U (2018) "Green biosynthesis of CuO and Ag–CuO nanoparticles from Malus domestica leaf extract and evaluation of antibacterial, antioxidant and DNA cleavage activities. New J Chem 42(1):204–213

    Article  CAS  Google Scholar 

  • Jeyapratha J, Christina C, Chandra Lekha N (2022) Green synthesis of copper nanoparticles from the flower extract of Ipomea pes-caprae collected from Thoothukudi coastal areas.

  • Kalia A, Sharma SP, Vashisht VK (2017) Scanning electron microscopy study of root tissue of muskmelon: transferring Fusarium wilt resistance from snapmelon to muskmelon. J Appl Nat Sci 9(3):1317–1323

    Article  CAS  Google Scholar 

  • Khatami M, Nejad MS, Salari S, Almani PGN (2016) Plant-mediated green synthesis of silver nanoparticles using Trifolium resupinatum seed exudate and their antifungal efficacy on Neofusicoccum parvum and Rhizoctonia solani. IET Nanobiotechnol 10(4):237–243

    Article  PubMed  PubMed Central  Google Scholar 

  • Lee Y-J, Kim K, Shin I-S, Shin KS (2020) Antioxidative metallic copper nanoparticles prepared by modified polyol method and their catalytic activities. J Nanopart Res 22(1):1–8

    Article  CAS  Google Scholar 

  • Liu HF, Xue XJ, Yu Y, Xu MM, Lu CC, Meng XL, Zhang BG, Ding XH, Chu ZH (2020) Copper ions suppress abscisic acid biosynthesis to enhance defence against Phytophthora infestans in potato. Mol Plant Pathol 21(5):636–651

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • López-Vargas ER, Ortega-Ortíz H, Cadenas-Pliego G, de Alba Romenus K, Cabrera de la Fuente M, Benavides-Mendoza A, Juárez-Maldonado A (2018) Foliar application of copper nanoparticles increases the fruit quality and the content of bioactive compounds in tomatoes. Appl Sci 8(7):1020

    Article  CAS  Google Scholar 

  • Mahfoudhi A, Salem AB, Garrab M, Hammami S, Gorcii M, Mastouri M, Mighri Z (2016) Antioxidant and antimicrobial activities of Tamarix aphylla (L.) Karst. growing in Tunisia. Mor. J. Chem. 4(4):2987–2995

    Google Scholar 

  • Malandrakis AA, Kavroulakis N, Chrysikopoulos CV (2019) Use of copper, silver and zinc nanoparticles against foliar and soil-borne plant pathogens. Sci Total Environ 670:292–299

    Article  CAS  PubMed  Google Scholar 

  • Mali SC, Raj S, Trivedi R (2020) Nanotechnology a novel approach to enhance crop productivity. Biochem Biophys Rep 24:100821

    Google Scholar 

  • Morsy MK, Elsabagh R, Trinetta V (2018) Evaluation of novel synergistic antimicrobial activity of nisin, lysozyme, EDTA nanoparticles, and/or ZnO nanoparticles to control foodborne pathogens on minced beef. Food Control 92:249–254

    Article  CAS  Google Scholar 

  • Mosa KA, El-Naggar M, Ramamoorthy K, Alawadhi H, Elnaggar A, Wartanian S, Ibrahim E, Hani H (2018) Copper nanoparticles induced genotoxicty, oxidative stress, and changes in superoxide dismutase (SOD) gene expression in cucumber (Cucumis sativus) plants. Front Plant Sci 9

  • Nadaroğlu H, Güngör AA, Ince S (2017) Synthesis of nanoparticles by green synthesis method. Int J Inn Res 1(1):6–9

    Google Scholar 

  • Nagajyothi P, Muthuraman P, Sreekanth T, Kim DH, Shim J (2017) Green synthesis: in-vitro anticancer activity of copper oxide nanoparticles against human cervical carcinoma cells. Arab J Chem 10(2):215–225

    Article  CAS  Google Scholar 

  • Nagar N, Devra V (2018) Green synthesis and characterization of copper nanoparticles using Azadirachta indica leaves. Mater Chem Phys 213:44–51

    Article  CAS  Google Scholar 

  • Nakhjavani M, Nikkhah V, Sarafraz M, Shoja S, Sarafraz M (2017) Green synthesis of silver nanoparticles using green tea leaves: experimental study on the morphological, rheological and antibacterial behaviour. Heat Mass Transf 53(10):3201–3209

    Article  CAS  Google Scholar 

  • Nishioka T, Marian M, Kobayashi I, Kobayashi Y, Yamamoto K, Tamaki H, Suga H, Shimizu M (2019) Microbial basis of Fusarium wilt suppression by Allium cultivation. Sci Rep 9(1):1715

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Ouda SM (2014a) Antifungal activity of silver and copper nanoparticles on two plant pathogens, Alternaria alternata and Botrytis cinerea. Res J Microbiol 9(1):34

    Article  Google Scholar 

  • Oumouloud A, El-Otmani M, Chikh-Rouhou H, Claver AG, Torres RG, Perl-Treves R, Alvarez J (2013) Breeding melon for resistance to Fusarium wilt: recent developments. Euphytica 192(2):155–169

    Article  CAS  Google Scholar 

  • Pariona N, Mtz-Enriquez AI, Sánchez-Rangel D, Carrión G, Paraguay-Delgado F, Rosas-Saito G (2019) Green-synthesized copper nanoparticles as a potential antifungal against plant pathogens. RSC Adv 9(33):18835–18843

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Polonio Á, Pineda M, Bautista R, Martínez-Cruz J, Pérez-Bueno ML, Barón M, Pérez-García A (2019) RNA-seq analysis and fluorescence imaging of melon powdery mildew disease reveal an orchestrated reprogramming of host physiology. Sci Rep 9(1):7978

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Quiterio-Gutiérrez T, Ortega-Ortiz H, Cadenas-Pliego G, Hernández-Fuentes AD, Sandoval-Rangel A, Benavides-Mendoza A, Cabrera-de la Fuente M, Juárez-Maldonado A (2019) The application of selenium and copper nanoparticles modifies the biochemical responses of tomato plants under stress by Alternaria solani. Int J Mol Sci 20(8):1950

    Article  CAS  PubMed Central  Google Scholar 

  • Sarkar J, Chakraborty N, Chatterjee A, Bhattacharjee A, Dasgupta D, Acharya K (2020) "Green synthesized copper oxide nanoparticles ameliorate defence and antioxidant enzymes in Lens culinaris. Nanomaterials 10(2):312

    Article  CAS  PubMed Central  Google Scholar 

  • Sebastiani MS, Bagnaresi P, Sestili S, Biselli C, Zechini A, Orrù L, Cattivelli L, Ficcadenti N (2017) Corrigendum: transcriptome analysis of the Melon-Fusarium oxysporum f sp melonis race 12 pathosystem in susceptible and resistant plants. Front Plant Sci 8:922

    Article  PubMed  PubMed Central  Google Scholar 

  • Shi W, Xiang L, Yu D, Gong S, Yang L (2020) Impact of the biofungicide tetramycin on the development of Fusarium head blight, grain yield and deoxynivalenol accumulation in wheat. World Mycotoxin J 13(2):235–246

    Article  CAS  Google Scholar 

  • Silvia Sebastiani M, Bagnaresi P, Sestili S, Biselli C, Zechini A, Orrù L, Cattivelli L, Ficcadenti N (2017) Transcriptome analysis of the melon-Fusarium oxysporum f sp melonis race 12 pathosystem in susceptible and resistant plants. Front Plant Sci 8:362

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Sindhu G, Murali M, Thriveni M, Anupama N, Amruthesh K (2018) Research article growth promotion and disease resistance in muskmelon induced by crude proteins of Penicillium verruculosum against gummy stem blight disease. Asian J Crop Sci 20180

  • Soto KM, Quezada-Cervantes CT, Hernández-Iturriaga M, Luna-Bárcenas G, Vazquez-Duhalt R, Mendoza S (2019) Fruit peels waste for the green synthesis of silver nanoparticles with antimicrobial activity against foodborne pathogens. LWT 103:293–300

    Article  CAS  Google Scholar 

  • Sudantha I, Suwardji S, Aryana I, Pramadya I, Jayadi I (2020) The effect of liquid bio fungicides dosage Trichoderma spp against fusarium wilt diseases, growth and yield of onion. J Phys Conf Ser

  • Suleiman MHA (2019) "Ethnobotanical, phytochemical, and biological study of Tamarix aphylla and Aerva javanica medicinal plants growing in the Asir region Saudi Arabia. Trop Conserv Sci 12:1940082919869480

    Article  Google Scholar 

  • Sun Q, Li J, Le T (2018) Zinc oxide nanoparticle as a novel class of antifungal agents: current advances and future perspectives. Sci Total Environ 43:11209–11220

    Google Scholar 

  • Taheriniya S, Behboodi Z (2016) Comparing green chemical methods and chemical methods for the synthesis of titanium dioxide nanoparticles. Int J Pharm Sci Res 7(12):4927

    CAS  Google Scholar 

  • Vatankhah M, Saberi-Riseh R, Moradzadeh Eskandari M, Afzali H (2019) Evaluation of some fungicides for the control of Fusarium dry rot of potato. J Crop Prot 8(3):275–285

    Google Scholar 

  • Winans MJ, Gallagher JE (2020) Metallomic and lipidomic analysis of S. cerevisiae response to cellulosic copper nanoparticles uncovers drivers of toxicity. Metallomics 12(5):799–812

    Article  CAS  PubMed  Google Scholar 

  • Worrall EA, Hamid A, Mody KT, Mitter N, Pappu HR (2018) Nanotechnology for plant disease management. Agronomy 8(12):285

    Article  CAS  Google Scholar 

  • Yang S, Xiong X, Arif S, Gao L, Zhao L, Shah IH, Zhang Y (2020) A calmodulin-like CmCML13 from Cucumis melo improved transgenic Arabidopsis salt tolerance through reduced shoot's Na+, and also improved drought resistance. Plant Physiol Biochem.

  • Zeebaree SYS, Zeebaree AYS (2019) Green copper nanoparticles as oxidising catalysts for multi-component reactions for synthesis of 1, 3, 4-thiadiazole derivatives at ambient temperature. Results Mater 100001

  • Zhang M, Xu J, Ren R, Liu G, Yao X, Lou L, Xu J, Yang X (2021) Proteomic analysis of Fusarium oxysporum-induced mechanism in grafted watermelon seedlings. Front Plant Sci 12:69

    Google Scholar 

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Acknowledgements

We are thankful to Amir Hassan Shah for his help and guidance in characterization of nanoparticles. We thank Qamar Zaman and Asmat Nawaz for their help to analyze the data from XRD and FTIR.

Accession Number:

1. Thaumatin like protein (TLP) (MELO3C003144).

2. Auxin influx carrier protein (AUX) (MELO3C013367),

3. Pathogenesis related protein 1 (PR1) (MELO3C017497),

4. Lucine-rich repeat protein (LRR) (MEL03C023962).

Funding

The study was financial supported by Shanghai Science and Technology Commission (20392000300), Shanghai Agriculture Applied Technology Development Program (No. 20180203).

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Authors and Affiliations

  1. School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, People’s Republic of China

    Iftikhar Hussain Shah, Kashif Hayat, Samiah Arif, Irfan Ali Sabir, Qingliang Niu & Yidong Zhang

  2. Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, People’s Republic of China

    Muhammad Ashraf

  3. Zhejiang Key Lab of Crop Germplasm, Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, People’s Republic of China

    Ali Raza Khan

  4. Queensland Alliance for Agriculture and Food Innovation, University of Queensland, Brisbane, 4072, Australia

    Muhammad Abdullah

  5. School of Life Sciences, Anhui Agricultural University, Hefei, 230036, People’s Republic of China

    Muhammad Aamir Manzoor

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  1. Iftikhar Hussain Shah
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  2. Muhammad Ashraf
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  3. Ali Raza Khan
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  4. Muhammad Aamir Manzoor
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  5. Kashif Hayat
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  6. Samiah Arif
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  7. Irfan Ali Sabir
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  8. Muhammad Abdullah
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  9. Qingliang Niu
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  10. Yidong Zhang
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Contributions

YZ supervised and designed the research. HIS designed and performed most of the experiments. MA revised the manuscript. ARK help in SEM and TEM analysis. KH, MAM helped in antifungal and DNA fragmentation assay. SA, IAS and MA assisted in RNA extraction, qRT-PCR and data analysis. QN and YZ revised, discussed and finalized the manuscript.

Corresponding author

Correspondence to Yidong Zhang.

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Shah, I.H., Ashraf, M., Khan, A.R. et al. Controllable synthesis and stabilization of Tamarix aphylla-mediated copper oxide nanoparticles for the management of Fusarium wilt on musk melon. 3 Biotech 12, 128 (2022). https://doi.org/10.1007/s13205-022-03189-0

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