Abstract
Self-nanoemulsifying drug delivery system (SNEDDS) containing aqueous leaf extracts of Justicia adhatoda (JA) and Psidium guajava (PG), was designed to evaluate their ability to enhance platelet count. The ternary phase diagram was constructed to identify the self-emulsifying regions and nanoemulsion prepared using clove oil, tween 80 and transcutol. The globule size of the prepared SNEDDS formulation at different folds of dilution in buffers of various pH was evaluated. The average globule size of the optimized JA2- and PG2-loaded SNEDDS at different folds of dilutions was in the range of 103.4 ± 7.1 to 238.3 ± 5.4 nm and 240.2 ± 7.9 to161.7 ± 3.7 nm, respectively. The viscosity of JA2 and PG2 loaded SNEDDS formulations were found to be 621 and 642 centipoise, respectively with no observed signs of phase separation, turbidity or precipitation during the freeze–thaw process. Oral administration of combined JA2 and PG2 loaded SNEDDS formulation to Wistar rats depleted with platelets showed a significant increase in platelet count when compared to a marketed tablet Caripill. Pharmacodynamic studies in platelet-depleted Wistar rats enhanced the platelet count after administration of SNEDDS containing lyophilized aqueous extracts JA2 and PG2.
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Acknowledgements
The authors would like to thank PSG Sons and Charities for providing all the facilities required to perform this work. We also thank Dr. Sivaram Hariharan, for his extensive English proofreading and editing services.
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Conceptualization: [VS] and [GS]; Methodology: [GS]; Formal analysis and investigation: [VS], [GS], [GV] and [GS]; Writing—original draft preparation: [AK] and [KS]; Writing—review and editing: [DB] and [VS]; Funding acquisition: [GS]; Resources: [GS]; Supervision: [VS].
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The studies were carried out according to the guidelines of the Council for the Purpose of Control and Supervision of Experiments on Animals, Ministry of Social Justice and Empowerment, Government of India, and the study protocol was carried out after obtaining the consent from PSG Institutional Animal Ethical Committee with registration no: 158/PO/ReBi/SL/99/CPCSEA.
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Saipriya, G., Karuppaiah, A., Syamala, G. et al. Self-nanoemulsifying drug delivery system of aqueous leaf extracts of Justicia adhatoda and Psidium guajava to enhance platelet count. 3 Biotech 12, 124 (2022). https://doi.org/10.1007/s13205-022-03164-9
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DOI: https://doi.org/10.1007/s13205-022-03164-9