Abstract
Pectin is one of the most important components of the plant cell wall. Galacturonosyltransferase-like (GATL) is an important enzyme involved in forming pectin in Arabidopsis thaliana. In this study, 12 PtGATL genes were identified and characterized based on the Populus trichocarpa genome using bioinformatics methods. The results showed that the PtGATLs contained four typical motifs, including DXD, LPPF, GLG, and HXXGXXKPW. According to phylogenetic analysis, PtGATLs were divided into six groups. Chromosome distribution and genome synteny analysis showed that there were 11 segmental-duplicated gene pairs with repeated fragments on chromosomes 2, 5, 7, 8, 10, and 14. Tissue-specific expression profiles indicated that these PtGATLs had different expression patterns. The transcription level of PtGATLs was regulated by different carbon dioxide and nitrogen concentrations. In conclusion, the identification and analysis of PtGATL genes in poplar provide important information on the gene function.
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Acknowledgements
This work was supported by the Science Fund Project of Heilongjiang Province of China (ZD2020C004), the Special Fund for Basic Scientific research operation Fee of Central University (2572017EA05), and the Innovation Project of State Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University) (2019A03).
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ZX and CQ conceived and designed the study, JS performed most of the experiments, SZ, CX and RC conducted the sampling, SZ, and JS performed bioinformatics calculations, CX and RC processed and analyzed the data, and JS, CQ, GL, C Y and ZX wrote the manuscript.
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13205_2022_3129_MOESM1_ESM.jpg
Supplementary file1 Fig. S1 GO analysis of the PtGATL genes. The gene ontologies were predicted for the categories of cellular components and molecular functions (JPG 177 KB)
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Supplementary file2 Fig. S2 Chromosomal distribution of PtGATL genes. The chromosome numbers and gene names were presented in the upper and both sides of red line, respectively. The red line represented the chromosomes, and the left most scale indicated the chromosome length (Mb) (JPG 39 KB)
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Suo, J., Zhang, S., Xu, C. et al. Identification and expression analysis of the PtGATL genes under different nitrogen and carbon dioxide treatments in Populus trichocarpa. 3 Biotech 12, 67 (2022). https://doi.org/10.1007/s13205-022-03129-y
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DOI: https://doi.org/10.1007/s13205-022-03129-y