Abstract
Copy number variations (CNVs) belong to mutations in the genome level with loci in the region of genic or intergenic. It is through different effects (such as position effect and dose effect) that influence complex traits and diseases. Deleted in Malignant Brain Tumors 1 (DMBT1) gene is a member of the scavenger receptor cysteine-rich super family. In cattle, this gene has been associated with the susceptibility to bovine tuberculosis. In this study, a new CNV was found in DMBT1 gene of Chinese cattle breeds and tested in two different Chinese cattle breeds (Jiaxian red and Pinan) for frequency distribution analysis. Besides, the body size data such as body length, body height, chest girth, chest width, rump length, and rump girth for Jiaxian (JX) and Pinan (PN) cattle were collected and associated with the newly identified CNV. The CNV was significantly associated with the body length and chest girth of JX cattle, and the rump length of PN cattle (P < 0.05). Furthermore, the expression profile of the DMBT1 gene was tested in calves’ tissues and the myoblasts differentiation. It was found that the DMBT1 gene expression was high in tuberculosis susceptible tissues (liver and lungs) at the calf stage and high in myoblast early differentiation. These tests were done using the qPCR method. As the result, the CNV of DMBT1 gene could be used as a candidate marker for bovine growth and health in marker-assisted selection (MAS) breeding.
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Acknowledgements
This research has been supported by the Henan Beef Cattle Industrial Technology System (No. S2013-08); Henan provincial modern agricultural industrial park (YUNONG plan2019-38); China Agriculture Research System of MOF and MARA (No. CARS-37); Science-Technology Foundation for innovation and creativity of Henan Academy of Agricultural Sciences (2020CX09).
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LZ and JX wrote the original draft. YZ reviewed and edited the manuscript. XL, ZZ, JZ, YW, ZY, PY, FC, WH, ZQ, GY, and CL provided the technical and material. EW, HC, BR, and YH directed and supervised the project.
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Animal care and study protocols were in accordance with the Animal Care Commission of the College of Veterinary Medicine, Northwest A&F University.
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Zheng, L., Xu, J., Liu, X. et al. The copy number variation of DMBT1 gene effects body traits in two Chinese cattle breeds. 3 Biotech 12, 93 (2022). https://doi.org/10.1007/s13205-022-03124-3
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DOI: https://doi.org/10.1007/s13205-022-03124-3