Abstract
A fluorescence and biosurfactant-producing strain HN3-2 was isolated from a rhizosphere soil sample of wheat plants and the chromosome of the strain HN3-2 was sequenced and was analyzed by multiple bioinformatics tools in this study. The genome size of the strain HN3-2 is 6,441,476 bp, with a GC content of 60.54%. 16Sr RNA-based phylogeny analysis showed that the strain HN3-2 belongs to Pseudomonas koreensis subgroup in Pseudomonas species. Preliminary data from genome mining have showed that the strain Pseudomonas sp. HN3-2 is capable of producing a peptide-type metabolite. Solid-phase extraction, reversed-phase high performance liquid chromatography (RP-HPLC) together with liquid chromatography–mass spectrometry, high-resolution mass spectrometry and tandem mass spectrometry analysis have led to the purification and identification of a cyclic lipopeptide (CLP) bananamide C (1) from the fermentative broth of the strain Pseudomonas sp. HN3-2. Moreover, the biological activity tests showed that banananmide 3 displays moderate antagonistic activity against Staphylococcus aureus and Escherichia coli. Collectively, these results provide the possibility of developing the CLP bananamide C as a drug leads for medical applications.
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Acknowledgements
The author acknowledges the grants from the National Natural Science Foundation of China (No. 32060625), the Natural Science Foundation of Gansu Province (No. 20JR5RA527) and the Project for Enhancing the Research Capability of Young Teachers in Northwest Normal University (No. NWNU-LKQN-18-26) for supporting this study.
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The first author conceived and designed the study, conducted the experiments, wrote the manuscript and finally approved the submission of the manuscript.
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Accession Number: The full genome of the strain Pseudomonas sp. HN3-2 has been released in NCBI database under the accession number of CP081200.
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Ma, Z. Analysis of the complete genome sequence of a rhizosphere-derived Pseudomonas sp. HN3-2 leads to the characterization of a cyclic lipopeptide-type antibiotic bananamide C. 3 Biotech 12, 35 (2022). https://doi.org/10.1007/s13205-021-03100-3
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DOI: https://doi.org/10.1007/s13205-021-03100-3