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Fabrication of a sensing platform for identification of tumor necrosis factor-alpha: a biomarker for neonatal sepsis

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Abstract

Neonatal sepsis is a prime cause of neonatal deaths across the globe. Presently, various medical tests and biodevices are available in neonatal care. These diagnosis platforms possess several limitations such as being highly expensive, time-consuming, or requiring skilled professionals for operation. These limitations can be overcome through biosensor development. This work discusses the assembling of an electrochemical sensing platform that is designed to detect the level of tumor necrosis factor-alpha (TNF-α). The sensing platform was moderated with nanomaterials molybdenum disulfide nanosheets (MoS2NSs) and silicon dioxide-modified iron oxide nanoparticles (Fe3O4@SiO2NPs). The integration of nanomaterials helps in accomplishing the improved characteristics of the biosensor in terms of conductivity, selectivity, and sensitivity. Further, the molecularly imprinted polymer (MIP) approach was incorporated for sensing the presence of TNF-α on the surface of the working electrode. The electrochemical response of the electrode was recorded at different conditions. A broad concentration range was selected to optimize the biosensor from 0.01 pM to 100 nM. The sensitivity of the biosensor was higher and it exhibits a lower detection limit (0.01 pM).

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Abbreviations

TNF-α:

Tumor necrosis factor-alpha

EOS:

Early-onset sepsis

MoS2NSs:

Molybdenum disulfide nanosheets

Fe3O4@SiO2NPs:

Silicon dioxide-modified iron oxide nanoparticles

DPV:

Differential pulse voltammetry

MIPtemplate :

Molecularly imprinted polymer with template

LOS:

Late-onset sepsis

SPE:

Screen-printed electrode

HCl:

Hydrochloric acid

DW:

Distilled water

Fe3O4 :

Iron oxide

NIP:

Non-imprinted polymer

SWV:

Square wave voltammetry

BET:

Brunauer–Emmett–Teller

CV:

Cyclic voltammetry

LOD:

Limit of detection

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Funding

The work was financially supported by the Extramural Research Grant (File No. EMR/2016/007564) and Young Scientist Scheme (File No. YSS/2015/000023) of the Science and Engineering Research Board, Government of India, and Technology Development Program (TDP) (TDP/BDTD/33/2019) of Department of Science and Technology (DST), Government of India, and by Department of Biotechnology (DBT) (BT/PR36874/MED/97/475/2020), Government of India.

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Authors and Affiliations

  1. Amity Institute of Nanotechnology (AINT), Amity University Uttar Pradesh (AUUP), Sector-125, Noida, 201313, India

    Sapna Balayan, Nidhi Chauhan & Utkarsh Jain

  2. Amity Institute of Biotechnology (AIB), Amity University Uttar Pradesh (AUUP), Sector-125, Noida, 201313, India

    Prabhanshu Kumar

  3. Drug Discovery and Development Laboratory, Department of Chemistry, University of Delhi, Delhi, 110007, India

    Ramesh Chandra

  4. Institute of Nanomedical Sciences (INMS), University of Delhi, Delhi, 110007, India

    Ramesh Chandra

Authors
  1. Sapna Balayan
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  2. Nidhi Chauhan
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  3. Prabhanshu Kumar
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  4. Ramesh Chandra
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  5. Utkarsh Jain
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Correspondence to Ramesh Chandra or Utkarsh Jain.

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Balayan, S., Chauhan, N., Kumar, P. et al. Fabrication of a sensing platform for identification of tumor necrosis factor-alpha: a biomarker for neonatal sepsis. 3 Biotech 12, 37 (2022). https://doi.org/10.1007/s13205-021-03083-1

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