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Functional properties and potential application of ethanol tolerant β-glucosidases from Pichia ofunaensis and Trichosporon multisporum yeasts

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Abstract

β-Glucosidases have been extensively investigated to integrate the enzyme complex for cellulose fiber saccharification and for improving the aroma of wine. To produce these enzymes, greater attention has been given to filamentous fungi and bacteria, and few investigations have targeted the potential applications of enzymes secreted by yeasts. Addressing this issue, in this study, β-glucosidases were produced by the Pichia ofunaensis and Trichosporon multisporum yeasts, via solid state fermentation with wheat bran as a substrate. When using p-Nitrophenyl β-d-glucopyranoside (pNPG) as an enzyme substrate, maximum β-glucosidase activities were detected at pH 5.5–6.0 and 50–60 °C for P. ofunaensis, and pH 5–6 and 55 °C for T. multisporum. Both enzymes were able to hydrolyze cellobiose and exhibited stability over a wide range of pH (3.5–9.0) for 24 h at 4 °C, thermostability up to 50 °C for 1 h and tolerance to 10 mM phenolic compounds. Negative modulation on enzyme activity was observed in the presence of Cu2+, Fe3+, Zn2+, Al3+ and Hg2+, while both β-glucosidases were tolerant to 30% methanol, isopropanol and acetone. In the presence of ethanol and glucose, enzymes from P. ofunaensis were the more active and stable of the two. These enzymes, especially the P. ofunaensis β-glucosidases, could be tested in enology for improving the aroma of wine and for integrating a cellulolytic complex to produce 2G ethanol.

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Acknowledgements

The authors would like to acknowledge the financial support provided by Fundação de Amparo à Pesquisa do Estado de São Paulo-FAPESP (processes 2017/06399-3, 2018/07036-4 and 2017/06066-4).

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Authors and Affiliations

  1. Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista “Júlio de Mesquita Filho”, R/Cristóvão Colombo, 2265, Jd Nazareth, Ibilce-Unesp, São José do Rio Preto, São Paulo, Brazil

    Jaqueline Elaine Vaz, Lacan Rabelo, Mohammed Anas Zaiter, Waldir Eduardo Simioni Pereira, Gustavo Metzker, Maurício Boscolo, Roberto da Silva, Eleni Gomes & Ronivaldo Rodrigues da Silva

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  1. Jaqueline Elaine Vaz
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  2. Lacan Rabelo
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  3. Mohammed Anas Zaiter
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  4. Waldir Eduardo Simioni Pereira
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  5. Gustavo Metzker
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  6. Maurício Boscolo
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  7. Roberto da Silva
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  8. Eleni Gomes
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  9. Ronivaldo Rodrigues da Silva
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Correspondence to Ronivaldo Rodrigues da Silva.

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Vaz, J.E., Rabelo, L., Zaiter, M.A. et al. Functional properties and potential application of ethanol tolerant β-glucosidases from Pichia ofunaensis and Trichosporon multisporum yeasts. 3 Biotech 11, 467 (2021). https://doi.org/10.1007/s13205-021-03027-9

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