Abstract
Enzymes from phytopathogenic fungi are desirable for biotechnological applications and a highly virulent phytopathogen shows great appeal for enzymes production. To assess the biotechnological potential of Kretzschmaria zonata, a plant pathogenic fungus, we analyzed its enzymatic profile after growth on six different types of lignocellulosic biomasses. The fungus was able to produce a wide variety of enzymes with superior xylanase activity. The corn cob induced the highest specific activity of xylanase, 56.30 U/mg of protein, as well as other important enzymatic activities such as endoglucanase, 11.20 U/mg of protein; pectinase, 4.52 U/mg of protein; and β-glucosidase, 2.77 U/mg of protein. The highest release of xylose, 0.88 g/L, was observed after saccharification of 10% of alkaline pretreated sugarcane bagasse by a commercial cocktail supplemented with the crude extract from K. zonata after growth on corn cob. The fungus extract is rich in hemicellulases and accessory enzymes and the result showed synergism between the enzymes present in the commercial mixture and in the K. zonata extract. This is the first report concerning the biotechnological potential of the fungus K. zonata, especially regarding to its ability to produce plant biomass degrading enzymes related to second generation ethanol production.
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Acknowledgements
The authors thank Fundação de Amparo à Pesquisa do Estado de Mato Grosso (FAPEMAT 0211243/2017), and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) for financial support of the work.
Funding
The authors thank Fundação de Amparo à Pesquisa do Estado de Mato Grosso FAPEMAT (0211243/2017), and CAPES for financial support of the work. This work was supported by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Grant no. 001).
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All authors contributed to the study conception and design. Material preparation, data collection, and analysis were performed by MLM, LFA, RISLÀ, and GPM-A. The first draft of the manuscript was written by GPM-A, RFA, and VMG and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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da Luz Morales, M., Almeida, L.F., de Souza Ladeira Àzar, R.I. et al. First report on the enzymatic profile of Kretzschmaria zonata. 3 Biotech 11, 398 (2021). https://doi.org/10.1007/s13205-021-02940-3
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DOI: https://doi.org/10.1007/s13205-021-02940-3