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MicroRNA-199-3p targets Sp1 transcription factor to regulate proliferation and epithelial to mesenchymal transition of human lung cancer cells

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Abstract

The present study was undertaken to study the function of miRNA-199-3p in the regulation of human lung cancer growth and metastasis. The results showed significant (P < 0.05) downregulation of miRNA-199-3p in lung cancer tissues and cell lines. Overexpression of miR-197 caused considerable inhibition of the viability and colony formation of the lung cancer cells. The inhibition of proliferation was found to be due to the arrest of the SK-LU-1 lung cancer cells. At the G2/M phase of the cell cycle. In silico analysis and subsequent the dual-luciferase assays showed that miR-199-3p targets Sp1 at molecular. The expression of Sp1 was significantly (P < 0.05) upregulated in lung cancer cells and tissues. Nonetheless, miR-199-3p overexpression could cause post-transcriptional suppression of Sp1. Silencing of Sp1suppress the proliferation of SK-LU-1 lung cancer cells. However, overexpression Sp1 transcription factor prevents the tumor-suppressive effects of miR-199-3p on lung cancer cells. Additionally, miR-199-3p was found to suppresses the migration, invasion and epithelial-to-mesenchymal transition of human lung cancer cells. Summing up, miRNA-199-3p/SP1 axis controls the growth and metastasis of SK-LU-1 lung cancer cells.

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    Authors and Affiliations

    1. Department of Pulmonary Medicine, The Second People’s Hospital of Yueqing, Zhejiang, 325608, China

      Jiajia Fu & Bin Xia

    2. Department of Gastroenterology and Hepatology, Zhongshan Hospital, Fudan University, Shanghai, 200032, China

      Tong Li

    3. Department of Pulmonary Medicine, PingYang Hospital Affiliated to Wenzhou Medical University, Zhenjiang, 325400, China

      Xiaozhen Jiang

    4. Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, No.180, Fenglin Road, Xuhui District, Shanghai, 200032, China

      Lijuan Hu

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    1. Jiajia Fu
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    2. Tong Li
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    3. Xiaozhen Jiang
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    4. Bin Xia
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    Contributions

    Conceptualization: JF and LH; methodology: JF, TL, XJ, BX and LH; formal analysis and investigation: JF and TL; writing—original draft preparation: JF and LH; writing—review and editing critically for important intellectual content: LH; supervision: LH.

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    Correspondence to Lijuan Hu.

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    All the authors declare that there is no conflict of interest.

    Ethical approval

    Informed consent was sought from the patients before inclusion in the present study. The research ethics committee of Zhongshan Hospital, Fudan University, Shanghai, China. Approved the study with approval number ZHF0172/2020.

    Additional information

    Jiajia Fu and Tong Li contributed to this work equally.

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    Fu, J., Li, T., Jiang, X. et al. MicroRNA-199-3p targets Sp1 transcription factor to regulate proliferation and epithelial to mesenchymal transition of human lung cancer cells. 3 Biotech 11, 352 (2021). https://doi.org/10.1007/s13205-021-02881-x

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    • DOI: https://doi.org/10.1007/s13205-021-02881-x

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