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NF-κB inhibitory, antimicrobial and antiproliferative potentials of compounds from Hawaiian fungus Aspergillus polyporicola FS910

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Abstract

Bioassay-guided experimental design and chromatographic analysis led to the isolation and identification of ten compounds (1–10) including two unusual sulfur-containing curvularin macrolides (1 and 2) from a Hawaiian fungal strain Aspergillus polyporicola FS910. Compounds 1 and 2 are rare curvularin macrolides each with a five-membered cyclic sulfur-containing moiety. The structures of the compounds were identified by HRESIMS, NMR spectroscopy, X-ray crystallography, ECD and DFT energy calculation, as well as comparing with previous literatures. Compounds 4, 6 and 8 were active against TNF-α-induced NF-κB inhibitory activity with IC50 values of 26.45, 5.41 and 15.8 µM, respectively. Compounds 3 and 5–8 exhibited anti-proliferative activity against HT1080, T46D, and A2780S cell lines, with IC50 values ranging from 2.48 to 29.17 μM. Additionally, Compound 3 showed promising antimicrobial activity against methicillin-resistant Staphylococcus aureus (MRSA), Bacillus subtilis, Escherichia coli and Candida albicans. Moreover, when tested in combination with antibiotic adjuvant disulfiram [4 µg/mL], compounds 4, 5 and 10 also displayed significant antibacterial activity against S. aureus.

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Accession Number: GenBank (Accession Number CCDC 2026987 and CCDC 2026988).

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Acknowledgements

This work was financially supported by start-up funding from Daniel K. Inouye College of Pharmacy (DKICP), Seed Grants from University of Hawaii at Hilo (UH Hilo), and the Victoria S. and Bradley L. Geist Foundation (15ADVC-74420, 17CON-86295, and 20CON-102163) (to SC). Funding for this work was also supported by Hawaii IDeA Network for Biomedical Research Excellence III and IV (INBRE-III and INBRE-IV) project: NIGMS Grant 5P20GM103466. We would also like to express our gratitude to Mr. Justin Reinicke for his help with HRMS, NMR, optical rotation and ECD data collection.

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Authors and Affiliations

  1. Department of Pharmaceutical Sciences, Daniel K. Inouye College of Pharmacy, University of Hawai’i at Hilo, Hilo, HI, 96720, USA

    Cong Wang, K. H. Ahammad uz Zaman, Xiaohua Wu & Shugeng Cao

  2. Guangxi Key Laboratory of Chemistry and Engineering of Forest Products, Guangxi Collaborative Innovation Center for Chemistry and Engineering of Forest Products, School of Chemistry and Chemical Engineering, Guangxi University for Nationalities, Nanning, 530006, People’s Republic of China

    Cong Wang

  3. Instituto de Química Rosario (CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, 2000, Rosario, Argentina

    Ariel M. Sarotti

  4. Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA, 02138, USA

    Shao-Liang Zheng

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  1. Cong Wang
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  2. K. H. Ahammad uz Zaman
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  3. Ariel M. Sarotti
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  4. Xiaohua Wu
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  6. Shugeng Cao
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Correspondence to Shugeng Cao.

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Wang, C., Zaman, K.H.A.u., Sarotti, A.M. et al. NF-κB inhibitory, antimicrobial and antiproliferative potentials of compounds from Hawaiian fungus Aspergillus polyporicola FS910. 3 Biotech 11, 391 (2021). https://doi.org/10.1007/s13205-021-02877-7

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