Abstract
A new matrix formulation was devised for catalase immobilization. Carrageenan-alginate beads different ratios were developed and soaked into different ratios of CaCl2–KCl as a hardening solution. The best formulation for loading capacity was selected, treated with polyethylene imine followed by glutaraldehyde and further studied. The best concentration of catalase for immobilization was 300U/ml and the best loading time was 6 h. The catalytic properties increased after immobilization and the immobilized catalase achieved optimum activity at a temperature range of 30–50 °C that was compared to the optimum activity of free catalase which occurred at 40 °C. Higher catalytic activity of immobilized catalase occurred at alkaline pHs than the free one which achieved optimum catalytic activity at neutral pH. A comparison between the kinetic parameters of immobilized and free catalase showed variation. The KM and Vmax of the immobilized catalase were 2.4 fold and six times higher than those of free catalase. The results of the study indicate that the formulated matrix can be used as a good matrix for catalase enzyme in various industrial applications.
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Acknowledgements
The authors would like to gratefully aknowledge Dr/Mohamed Elsayed Ali Hassan, Dr/Mohamed Ahmed Hussein Yassin, Dr/Roqaya Ibrahim Bassuny and Dr/Asmaa El-Shershaby for their scientific and technical support.
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This work received no external funding. This work was funded internally by the National Research Centre and is a part of the doctoral thesis of Ali O. Ali.
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Ali, A.O., Abdalla, M.S., Shahein, Y.E. et al. Grafted carrageenan: alginate gel beads for catalase enzyme covalent immobilization. 3 Biotech 11, 341 (2021). https://doi.org/10.1007/s13205-021-02875-9
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DOI: https://doi.org/10.1007/s13205-021-02875-9