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Impact of chitosan seed treatment of fenugreek for management of root rot disease caused by Fusarium solani under in vitro and in vivo conditions

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Abstract

The use of chitosan as an alternative for fungicides has received more attention worldwide. Hence, this study aimed to evaluate in vitro and in vivo antifungal activity of chitosan against Fusarium solani causing root rot in fenugreek. Chitosan treatments ranged from 0.1 to 2gL−1 were tested against F. solani on to potato dextrose agar and in potato dextrose broth. The results revealed that increase in concentrations of chitosan significantly reduced growth, dried biomass, sporulation and spore germination of F. solani. The hyphal swellings and distortion of F. solani mycelia were induced by chitosan. Fenugreek seeds treated with chitosan at 2 gL−1 and 0.5 gL−1 showed reduced F. solani infection and increased seed germination, respectively. In pot and field studies, fenugreek seeds treated with chitosan at 2.0 gL−1 greatly reduced root rot disease severity and also enhanced yield parameters. The activity of defence enzymes, such as chitinase, β-1, 3-glucanase and total phenol were increased in chitosan treated in fenugreek plants. This increased activity offered protection to fenugreek plants against F. solani to a greater extent. The results showed that chitosan could be used as inducer of defense response and has the potential of controlling fenugreek root rot disease.

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References

  • Abd-El-Kareem F, Saied NM, El-Mohamedy RSR (2018) Seed treatment with chitosan and ethanol-extracted Propolis for suppression bean root rot disease under greenhouse conditions. J Mater Environ Sci 9:2356–2361

    CAS  Google Scholar 

  • Abo-Elyousr KAM, Zein E-A, Hassan MHA, El-Sheakh MM (2014) Enhance suppressive effect of compost on soybean Rhizoctonia root rot by soil treatment with Trichoderma harzianum. J Plant Physiol Pathol 2:2. https://doi.org/10.4172/2329-955X.1000122

    Article  Google Scholar 

  • Ainsworth EA, Gillespie KM (2007) Estimation of total phenolic and other oxidation substrates in plant tissues using Folin–Ciocalteu reagent. Nat Protoc 2:875–877

    CAS  PubMed  Google Scholar 

  • Al-Hetar MY, ZainalAbidin MA, Sariah M, Wong MY (2011) Antifungal activity of chitosan against Fusarium oxysporum f. sp. cubense. J Appl Polym Sci 120:2434–2439

    CAS  Google Scholar 

  • Ali MZH, El-Sheikh Aly MM, Ghaly HMA, Abd-Elaziz MA (2018) Biological control of damping-off and root rot of fenugreek. J Phytopathol Pest Manag 5:59–75

    Google Scholar 

  • Avdiushko SA, Ye XS, Kuc J (1993) Detection of several enzymatic activities in leaf prints cucumber plants. Physiol Mol Plant Pathol 42:441–454

    CAS  Google Scholar 

  • Bautista-Banos S, Hernandez-Louzardo AN, Velazquez-del Valle MG, Hernandez-Lopez M, Ait BE, Bosquez-Molina E, Wilson CL (2006) Chitosan as a potential natural compound to control pre and postharvest diseases of horticultural commodities. Crop Prot 25:108–118

    CAS  Google Scholar 

  • Benhamou N, Kloepper JW, Tuzunm S (1998) Induction of resistance against Fusarium wilt of tomato by combination of chitosan with an endophytic bacteria strain: ultrastructure and cytochemistry of the host response. Planta 204:153–168

    CAS  Google Scholar 

  • Bhaskara Reddy MV, Arul J, Angers P, Couture L (1999) Chitosan treatment of wheat seeds induces resistance to Fusarium graminearum and improves seed quality. J Agric Food Chem 47:1208–1216

    CAS  PubMed  Google Scholar 

  • Bhattacharya A (2013) Fungicidal potential of chitosan against phytopathogenic Fusarium solani. J Exp Biol Agric Sci 1:259–263

    Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    CAS  Google Scholar 

  • Cavalcanti FR, Resende MLV, Carvalho CPS, Silveira JAG, Oliveira JTA (2007) An aqueous suspension of Crinipellis perniciosa mycelium activates tomato defense responses against Xanthomonas vesicatoria. Crop Prot 26:729–738

    Google Scholar 

  • Chandrasekaran M, Belachew ST, Yoon E, Chun SC (2017) Expression of β-1,3-glucanase (GLU) and phenylalanine ammonia-lyase (PAL) genes and their enzymes in tomato plants induced after treatment with Bacillus subtilis CBR05 against Xanthomonas campestris pv. vesicatoria. J Gen Plant Pathol 83:7–13

    CAS  Google Scholar 

  • Dai DH, Hu WL, Huang GR, Li W (2011) Purification and characterization of a novel extracellular chitinase from thermophilic Bacillus sp. Hu1. Afr J Biotech 10:2476–2485

    CAS  Google Scholar 

  • El-Sawy NM, Hassan El-Rehim HAA, Elbarbary AM, Hegazy EA (2010) Radiation-induced degradation of chitosan for possible use as a growth promoter in agricultural purposes. Carbohyd Polym 79:555–562

    CAS  Google Scholar 

  • El-Tantawy EM (2009) Behaviour of tomato plants as affected by spraying with chitosan and aminofort as natural stimulator substances under application of soil organic amendments. Pak J Biol Sci 12:1164–1173

    CAS  PubMed  Google Scholar 

  • Fitza KN, Payn KG, Steenkamp ET, Myburg AA, Naidoo S (2013) Chitosan application improves resistance to Fusarium circinatum in Pinus patula. S Afr J Bot 85:70–78

    CAS  Google Scholar 

  • Gavhane YN, Gurav AS, Yadav AV (2013) Chitosan and its applications: a review of literature. Int J Res Pharm Biomed Sci 4:312–331

    Google Scholar 

  • Goy RC, Britto DD, Assis OB (2009) A review of the antimicrobial activity of chitosan. Polimeros 19:241–247

    CAS  Google Scholar 

  • Hadwiger LA, Loschk DC (1981) Molecular communication in host-parasite interactions: hexosamine polymers (chitosan) as regulator compounds in race-specific and other interactions. Phytopathology 71:756–762

    CAS  Google Scholar 

  • Hassan O, Chang T (2017) Chitosan for eco-friendly control of plant disease. Asian J Plant Pathol 11:53–70

    Google Scholar 

  • Hernandez-Lauzardo AN, Velazquez-del Valle MG, Guerra-Sanchez MG (2011) Current status of action mode and effect of chitosan against phytopathogens fungi. Afr J Microbiol Res 5:4243–4247

    CAS  Google Scholar 

  • Jabnoun-Khiareddine H, El-Mohamedy RSR, Abdel-Kareem F, Aydi Ben Abdallah R, Gueddes-Chahed M, Daami-Remadi M (2015) Variation in chitosan and salicylic acid efficacy towards soil-borne and air borne fungi and their suppressive effect of tomato wilt severity. J Plant Pathol Microbiol 6:325–334

    Google Scholar 

  • Kendra DF, Hadwiger LA (1984) Characterization of the smallest chitosan oligomer that is maximally antifungal to Fusarium solani and elicits pisatin formation by Pisum sativum. Exp Mycol 8:276–281

    CAS  Google Scholar 

  • Kumar MNR (2000) A review of chitin and chitosan applications. React Funct Polym 46:1–27

    CAS  Google Scholar 

  • Leuba JL, Stossel P (1986) Chitosan and other polyamines: antifungal activity and interaction with biological membranes. In: Muzzarelli R, Jeuniaux C, Graham GW (eds) Chitin in nature and technology. Plenum Press, New York, pp 215–222

    Google Scholar 

  • Lizarraga-Paulin EG, Torres-Pacheco I, Moreno-Martínez E, Miranda-Castro SP (2011) Chitosan application in maize (Zea mays) to counteract the effects of abiotic stress at seedling level. Afr J Biotech 10:6439–6446

    CAS  Google Scholar 

  • Mejdoub-Trabelsi B, Touihri S, Ammar N, Riahi A, Daami-Remadi M (2020) Effect of chitosan for the control of potato diseases caused by Fusarium species. J Phytopathol 168:18–27

    CAS  Google Scholar 

  • Mohammed SR, Zeitar EM, Eskov ID (2019) Inhibition of mycelia growth of Rhizoctonia solani by chitosan in vitro and in vivo. Open Agric J 13:156–161

    CAS  Google Scholar 

  • Mondal MMA, Malek MA, Puteh AB, Ismail MR, Ashrafuzzaman M, Naher L (2012) Effect of foliar application of chitosan on growth and yield in okra. Aust J Crop Sci 6:918–921

    CAS  Google Scholar 

  • Nelson N (1944) A photometric adaption of the Somogyi method for the determination of glucose. J Biol Chem 153:375–380

    CAS  Google Scholar 

  • Oliveira-Jr EN, El Gueddari NE, Moerschbacher BM, Franco TT (2012) Growth rate inhibition of phytopathogenic fungi by characterized chitosans. Braz J Microbiol 43:800–809

    Google Scholar 

  • Park SY, Marsh KS, Rhim JW (2002) Characteristics of different molecular weight chitosan film affected by the type of organic solvents. J Food Sci 67:194–197

    CAS  Google Scholar 

  • Photchanachai S, Singkaew J, Thamthong J (2006) Effects of chitosan seed treatment on Colletotrichum sp. and seedling growth of chilli cv. ‘Jinda.’ Acta Hortic 712:585–590

    Google Scholar 

  • Plascencia MG, Viniegra G, Olayo R, Castillo M, Shirai K (2003) Effect of chitosan and temperature on spore germination of Aspergillus niger. Macromol Biosci 3:582–586

    Google Scholar 

  • Ragab MM, Ashour AMA, Abdel-Kader MM, El-Mohamady R, Abdel-Aziz A (2012) In vitro evaluation of some fungicide’s alternatives against Fusarium oxysporum the causal of wilt disease of pepper (Capsicum annum L.). Int J Agric for 2:70–77

    Google Scholar 

  • Ramteke PK (2019) Effect of resistance inducers on in vitro inhibition of mycelial growth and sporulation of Fusarium solani causing root rot of fenugreek. Plant Pathol Quar 9:198–209

    Google Scholar 

  • Ramteke PK, Ghule MR, Ramteke SD (2019) First report of Fusarium solani causing root rot on fenugreek (Trigonella foenum-graecum L) in India. Plant Dis 104:992

    Google Scholar 

  • Rinaudo M (2006) Chitin and chitosan: properties and applications. Prog Polym Sci 31:603–632

    CAS  Google Scholar 

  • Ryals JA, Neuenschwander MG, Willits A, Molina A, Steiner HY, Hunt MD (1996) Systemic acquired resistance. Plant Cell 8:1809–1819

    CAS  PubMed  PubMed Central  Google Scholar 

  • Sarwar N, Zahid CMH, Ikramul HAQ, Jamil FF (2005) Induction of systemic resistance in chickpea against Fusarium wilt by seed treatment with salicylic acid and bion. Pak J Bot 37:989–995

    Google Scholar 

  • Tiru M, Muleta D, Bercha G, Adugna G (2013) Antagonistic effects of rhizobacteria against coffee wilt disease caused by Gibberella xylarioides. Asian J Plant Pathol 7:109–122

    Google Scholar 

  • Zivkovic S, Stevanovic M, Durovic S, Ristic D, Stosic S (2018) Antifungal activity of chitosan against Alternaria alternata and Colletotrichum gloeosporioides. Pestic Phytomed 33:197–204

    CAS  Google Scholar 

Download references

Acknowledgements

Authors are thanks to director of ICAR-National Research Centre for Grapes, Vasumitra Life Energies Pvt Ltd and principal of Raja Shripatrao Bhagawantrao Mahavidyalaya for permission of this work. Authors are also thanks to ISF chitin and Marine products LLP for suppling chitosan powder and Owner of Green Cultivate Agro Farm for providing land for field trial.

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NA.

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Authors and Affiliations

  1. Research and Development Division, Vasumitra Life Energies Pvt Ltd, Pune, Maharashtra, 411009, India

    Mahesh R. Ghule & Hemangee Jambhekar

  2. Department of Botany, Raja Shripatrao Bhagawantrao Mahavidyalaya, District Satara, Aundh, Maharashtra, 415510, India

    Purushottam K. Ramteke

  3. Plant Physiology, ICAR-National Research Centre for Grapes, Manjari Farm, Pune, Maharashtra, 412307, India

    Sahadeo D. Ramteke, Amruta Langote & Akshay V. Gaikwad

  4. Department of Biochemistry, KP Paramedical Institutes, Hadapsar, Pune, Maharashtra, 411028, India

    Prasad S. Kodre

  5. Plant Pathology, ICAR-National Research Centre for Grapes, Manjari Farm, Pune, Maharashtra, 412307, India

    Somnath K. Holkar

Authors
  1. Mahesh R. Ghule
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  2. Purushottam K. Ramteke
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  3. Sahadeo D. Ramteke
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  6. Akshay V. Gaikwad
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  8. Hemangee Jambhekar
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Contributions

MRG project in charge, research plan and monitoring all the experiment, manuscript writing. PKR in vitro and pot study activity of chitosan against Fusarium solani. SDR total phenol estimation. PSK enzyme activity estimation. AL field observation. SKH in vitro antagonistic activity, microscopic observation and Manuscript writing. HJ manuscript writing.

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Correspondence to Mahesh R. Ghule.

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Ghule, M.R., Ramteke, P.K., Ramteke, S.D. et al. Impact of chitosan seed treatment of fenugreek for management of root rot disease caused by Fusarium solani under in vitro and in vivo conditions. 3 Biotech 11, 290 (2021). https://doi.org/10.1007/s13205-021-02843-3

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