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Characterization of Dietzia maris AURCCBT01 from oil-contaminated soil for biodegradation of crude oil

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Abstract

A bacterial strain was isolated from an oil-contaminated site and on its’ further characterization, exhibited the potential of synthesising metabolites and the ability to degrade crude oil. Its’ morphological, biochemical and 16S rRNA analysis suggested that the bacterium belongs to Dietzia maris AURCCBT01. This strain rapidly grew in the medium supplemented with n-alkanes C14, C18, C20, C28 and C32 utilizing them as a sole carbon source and produced a maximum canthaxanthin pigment of 971.37 µg/L in the n-C14 supplemented medium and produced the lowest pigment yield of 389.48 µg/L in the n-C-32 supplemented medium. Moreover, the strain effectively degraded 91.87% of crude oil in 7 days. The emulsification activity of the strain was 25% with the highest cell surface hydrophobicity (70.26%) and it showed a decrease in surface tension, indicating that the biosurfactant production lowers the surface tension. This is the first report on the characterization of the strain, Dietzia maris AURCCBT01 and its’ novelty of alkane degradation and simultaneous production of canthaxanthin pigment.

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Acknowledgements

Dr. C.K. Venil thanks the UGC for awarding the Dr. D.S. Kothari Postdoctoral Fellowship (BL/17-18/0479). In addition, the authors thank Anna University, Regional Campus—Coimbatore for providing the necessary facilities to carry out the project work.

Funding

This work was supported and funded by the University Grants Commission (UGC), New Delhi under Dr. D.S. Kothari Post-doctoral Fellowship (BL/17-18/0479) dated 25 September 2018.

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Authors and Affiliations

  1. Department of Biotechnology, Anna University, Regional Campus-Coimbatore, Coimbatore, Tamil Nadu, 641046, India

    Chidambaram Kulandaisamy Venil & Ponnuswamy Renuka Devi

  2. Department of Chemistry, Bannari Amman Institute of Technology, Sathyamangalam, Tamil Nadu, 638401, India

    Mahalingam Malathi

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  1. Chidambaram Kulandaisamy Venil
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Correspondence to Chidambaram Kulandaisamy Venil.

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13205_2021_2807_MOESM1_ESM.jpg

Supplementary file1 (JPG 52 KB) (a) Ceric ammonium nitrate test for alcohol. (b) Fehling’s test for aldehyde. (c) Bicarbonate test for acid.

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Venil, C.K., Malathi, M. & Devi, P.R. Characterization of Dietzia maris AURCCBT01 from oil-contaminated soil for biodegradation of crude oil. 3 Biotech 11, 291 (2021). https://doi.org/10.1007/s13205-021-02807-7

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