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Prevalence of tumor necrosis factor alpha inducing protein (tipα) gene of Helicobacter pylori and its association with upper gastrointestinal diseases in India

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Abstract

Helicobacter pylori (H. pylori) is known to cause several gastroduodenal diseases including chronic Gastritis, Peptic Ulcer disease and Gastric Cancer. Virulent genes of H. pylori like cagA, vacA are known to be responsible for the disease pathogenesis. However, these virulence genes are not always found to be associated with disease outcome in all populations around the world. Tumor necrosis factor alpha inducing protein tipα is a newly discovered virulence gene of H. pylori and is an inducer of certain cytokines and chemokines that are responsible for causing stomach cancer. Therefore, we conducted a study, which aims to find the prevalence of tipα gene in the Indian patients with gastroduodenal symptoms, and its association with H. pylori related gastroduodenal diseases. 267 clinical H. pylori isolates are included in our study for finding the prevalence of tipα gene and its association with cagA and vacA gene using PCR assay. The current study shows that the prevalence rate of tipα gene is 59.9%. Our study has found a significant association (p < 0.05) of tipα gene with Non Ulcer Dyspepsia (NUD) and an association of cagA and vacAs1m1 with Gastritis and Duodenal Ulcer. Our study demonstrates for the first time the presence of tipα as virulence factor of H. pylori strain in Indian population isolated from patients suffering from gastroduodenal diseases. Further, tipα is significantly associated with NUD but not with other gastroduodenal diseases in India.

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Availability of data and materials

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

Abbreviations

NUD:

Non ulcer dyspepsia

H. pylori :

Helicobacter pylori

PUD:

Peptic ulcer disease

DU:

Duodenal ulcer

vacA :

Vacuolating cytotoxin A

cag PAI:

Cytotoxin-associated gene Pathogenicity Island

cagA:

Cytotoxin-associated gene A

NF-κb:

Nuclear factor Kappa B cells

TNF-α:

Tumor necrosis factor alpha

Tipα:

Tumor necrosis factor alpha inducing protein

GERD:

Gastro-esophageal reflux disease

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Acknowledgements

We thank Amity University for providing the infrastructure and support to carry out the work.

Funding

This study has been funded by ICMR (5/13/16/2015/NCD-III).

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Author notes

  1. Shweta Mahant and Shubham Mehra share equal authorship.

Authors and Affiliations

  1. Centre for Medical Biotechnology, Amity Institute of Biotechnology, Amity University, Noida, UP, India

    Shweta Mahant, Shubham Mehra, Ayushi Chhawchharia, Sudeep Bose & Rajashree Das

  2. Department of Health and Medical Science, University of Western, Perth, WA, Australia

    Ayushi Chhawchharia

  3. ICMR-National Institute of Cholera and Enteric Diseases, Kolkata, West Bengal, India

    Bipul Chandra Karmakar, Sangita Paul & Asish Kumar Mukhopadhyay

  4. Department of Gastroenterology, Manipal Hospitals, Dwarka, New Delhi, India

    Kunal Das

Authors
  1. Shweta Mahant
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  2. Shubham Mehra
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Contributions

RD and SB have given the concept of association between Tip α and gastric cancer. RD and AKM have finalized the manuscript. SM, SP, BCK and AC have done the experiments. SM has analyzed the data and written the manuscript. AKM has provided the samples and revised the manuscript. KD has conceptualized the idea clinically and critically corrected the manuscript.

Corresponding authors

Correspondence to Sudeep Bose or Rajashree Das.

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Conflict of interest

The authors declare that they have no competing interests.

Ethics approval and consent to participate

This study was approved by institutional ethical committee of Amity Institute of Biotechnology, Noida, UP, dated 2-12-2015 and National Institute of Cholera and Enteric Diseases, Kolkata (A-1/2016-IEC, dated 22-12-2016). Written informed consent was taken.

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Consent was taken from all participant.

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Mahant, S., Mehra, S., Chhawchharia, A. et al. Prevalence of tumor necrosis factor alpha inducing protein (tipα) gene of Helicobacter pylori and its association with upper gastrointestinal diseases in India. 3 Biotech 11, 246 (2021). https://doi.org/10.1007/s13205-021-02804-w

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  • DOI: https://doi.org/10.1007/s13205-021-02804-w

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