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Optimization of medium composition to increase the expression of recombinant human interferon-β using the Plackett–Burman and central composite design in E. coli SE1

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Abstract

Recombinant human interferon-β (rhIFN-β) is therapeutically important and new commercially viable approaches are needed for its increased production. In this study, a codon-optimized gene encoding for rhIFN-β(C17S) protein was designed and expressed in E. coli SE1. As a first step of medium optimization, growth of E. coli as a function of different media components was studied. Subsequently, to optimize the media composition, a response surface methodology (RSM) was used. Our results show that optimized medium (15.0 g/L tryptone, 12.3 g/L meat extract, 1.0 g/L MgSO4 and 0.5 g/L thiamine along with minimal medium) obtained in this study provide better growth of recombinant cells and the expression level of recombinant protein was ~ 1.7-fold more than Luria–Bertani medium. The optimized medium may be utilized for the large-scale production of rhIFN-β.

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Abbreviations

CCD:

Central composite design

rhIFN-β(C17S) :

Recombinant human interferon-beta variant containing C17S substitution

PB:

Plackett–Burman

LB medium:

Luria–Bertani medium

RSM:

Response surface methodology

IPTG:

Isopropyl-1-thio β-D galactopyranoside

Cys:

Cysteine

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Funding

This work was supported in partial by the research grants to AHP from the Department of Biotechnology (New Delhi) (BT/PR23283/MED/30/1953/2018) and NIPER, SAS Nagar (NPLC-AHP). DP and GP are thankful to the University Grants Commission and Department of Biotechnology, New Delhi, Govt. of India for support.

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Author notes

  1. Dharam Pal and Gopal Patel have contributed equally to this work.

Authors and Affiliations

  1. Department of Biotechnology, National Institute of Pharmaceutical Education and Research (NIPER), S.A.S. Nagar (Mohali), Sector 67, 160062, Punjab, India

    Dharam Pal, Prakashkumar Dobariya & Abhay H. Pande

  2. Department of Pharmaceutical Technology (Biotechnology), National Institute of Pharmaceutical Education and Research (NIPER), S.A.S. Nagar (Mohali), Sector 67, 160062, Punjab, India

    Gopal Patel & Uttam Chand Banerjee

  3. Laboratory of Medicinal Plant Biotechnology, College of Pharmacy, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, 310053, PR China

    Gopal Patel & Shivraj Hariram Nile

Authors
  1. Dharam Pal
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  2. Gopal Patel
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  3. Prakashkumar Dobariya
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  4. Shivraj Hariram Nile
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  5. Abhay H. Pande
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  6. Uttam Chand Banerjee
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Contributions

AHP and UCB were in-charge of the experiments and paper writing. DP, GP and PD performed experimental studies and participated in paper writing. SHN and GP performed review and editing. All the authors read and approved the final manuscript.

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Correspondence to Uttam Chand Banerjee.

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Pal, D., Patel, G., Dobariya, P. et al. Optimization of medium composition to increase the expression of recombinant human interferon-β using the Plackett–Burman and central composite design in E. coli SE1. 3 Biotech 11, 226 (2021). https://doi.org/10.1007/s13205-021-02772-1

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