Abstract
This study aimed to identify a symbiotic fungus strain HX-1 with anti-Vibrio harveyi activity and isolate and identify the active compound. The HX-1 strain was identified as Aspergillus fumigatus according to the morphological characteristics and internal transcribed spacer (ITS) sequence analysis. The compound was isolated from the fermentation product of HX-1 strain through ethyl acetate extraction, silica gel and Sephadex LH-20 column chromatography, and semi-preparative HPLC techniques using an antibacterial-guided fractionation method. According to its physicochemical properties and spectral characteristics, the compound was identified as trypacidin having the same anti-V. harveyi activity as streptomycin sulfate, with the minimum inhibitory concentration of 31.25 µg/mL.
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Acknowledgments
This work financially supported by the Natural Science Foundation of Jiangsu Provincial Department of Education (19KJB350007), Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), Open-end Funds of Jiangsu Key Laboratory of Marine Bioresources and Environment (SH20201207) and Postgraduate Research & Practice Innovation Program of Jiangsu Province (KYCX20_2896).
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Xu, X., Guo, S., Chen, H. et al. Bioassay-guided isolation and characterization of antibacterial compound from Aspergillus fumigatus HX-1 associated with Clam. 3 Biotech 11, 193 (2021). https://doi.org/10.1007/s13205-021-02754-3
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DOI: https://doi.org/10.1007/s13205-021-02754-3