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Microwave-assisted solvent-free extraction of essential oil from Coleus aromaticus: anti-phytopathogenic potential for fruit post-harvesting

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Abstract

This work evaluates the fungicidal effect of essential oil from Coleus aromaticus (C. aromaticus) by solvent-free microwave-assisted extraction with a yield of 0.54%. Fourier-transform infrared spectroscopy was utilised to identify the functional groups, which were O–H, C–O, C–H, and C=C. Gas chromatography–mass spectrometry analysis was performed to determine the primary essential oil components, namely, thymol (92.62%), thymoquinone (2.64%), creosol (1.77%), linalool (1.68%), p-Cymene-2,5-diol (0.73%), and p-Cymene (0.56%). The inhibitory effect of essential oil extracted from C. aromaticus against the isolated fungi, Aspergillus niger from mango, was investigated. The mycelial growth inhibition of the extracted essential oil by the poisoned food test and disc diffusion assay showed the reduction at 79.63 ± 1.7 and 70.45 ± 6.54%, respectively. In vivo experiment was conducted with artificially wounded and unwounded mangoes, applying the extracted essential oil to the wounded mangoes inoculated with A. niger that could decrease the disease incidence from 100 to 58.33 ± 14.43%. Meanwhile, the treatment of the extracted essential oil did not affect the quality of the mango and it also shows improvement in weight loss reduction of the mango in comparison to the chemical fungicide and untreated mango. Hence, essential oil from C. aromaticus by solvent-free microwave-assisted extraction could be used as an effective control for the fruit spoilage and potential source of fruit preservative.

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Acknowledgements

The authors would like to thank the Ministry of Education for financing the research under the Fundamental Research Grant Scheme (FRGS/1/2018/STG01/UNIMAP/03/3).

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Authors and Affiliations

  1. Faculty of Chemical Engineering Technology, Universiti Malaysia Perlis, Kompleks Pusat Pengajian Jejawi 3, Kawasan Perindustrian Jejawi, 02600, Arau, Perlis, Malaysia

    Ahmad Anas Nagoor Gunny, Lim Pei Fang, Subash C. B. Gopinath, Noor Hasyierah Mohd Salleh & Raja Hasnida Raja Hashim

  2. Phytochemistry Unit, Herbal Medicine Research Center, Institute for Medical Research, National Institute of Health, Selangor Darul Ikhsan, Malaysia

    Norazlan Mohmad Misnan

  3. Institute of Nano Electronic Engineering, Universiti Malaysia Perlis, 01000, Kangar, Perlis, Malaysia

    Subash C. B. Gopinath

  4. Institute of Sustainable Agrotechnology, Universiti Malaysia Perlis, 02100, Padang Besar, Perlis, Malaysia

    Mohd Hishamuddin Che Mat

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  1. Ahmad Anas Nagoor Gunny
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  2. Lim Pei Fang
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  3. Norazlan Mohmad Misnan
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  4. Subash C. B. Gopinath
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  5. Noor Hasyierah Mohd Salleh
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  6. Raja Hasnida Raja Hashim
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  7. Mohd Hishamuddin Che Mat
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The authors are contributed to the preparation of the manuscript and discussion. All authors read and approved the final manuscript.

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Correspondence to Ahmad Anas Nagoor Gunny.

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Gunny, A.A.N., Fang, L.P., Misnan, N.M. et al. Microwave-assisted solvent-free extraction of essential oil from Coleus aromaticus: anti-phytopathogenic potential for fruit post-harvesting. 3 Biotech 11, 166 (2021). https://doi.org/10.1007/s13205-021-02701-2

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  • DOI: https://doi.org/10.1007/s13205-021-02701-2

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