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Lactobacillus animalis pZL8a: a potential probiotic isolated from pig feces for further research

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Abstract

This study aimed to screen a potential anti-diarrheal probiotic for pigs to meet the growing demand for antibiotic alternatives in livestock. Six intestinal pathogens, Escherichia coli (O157: H7) ATCC 43888, Staphylococcus aureus ATCC 6538, Listeria monocytogenes ATCC 19115, Salmonella Typhimurium ATCC 14028, Shigella boydii ATCC 9207, and Staphylococcus haemolyticus ZSY2 were employed as indicator bacteria. Our result showed that Lactobacillus animalis pZL8a isolated from pig feces had extensive and higher antibacterial activity against indicator pathogens among 9 tested strains. In addition, valuable attributes of pZL8a such as great tolerance of low pH (3.0) and bile salts (0.3%), high-level adhesion to Caco-2 cells, and similar susceptibility to the reference strain Lactobacillus rhamnosus GG (LGG) were observed. Compared with control, pZL8a supplement significantly improved the level of immunoglobulin G (IgG), immunoglobulin M (IgM), and interleukin-2 (IL-2) in mouse serum. Therefore, L. animalis pZL8a was proposed as a potential probiotic for further research and hope to reduce or replace the application of antibiotics in animal production.

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Acknowledgements

This study was supported by the National Key R&D Program of China (2018YFD0501804, 2018YFD0502305) and NBCITS (CARS-37); State Key Laboratory of Veterinary Etiological Biology Project. We thank Edanz Group Ltd. for revising the manuscript.

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Authors and Affiliations

  1. State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Yanchangbu, Lanzhou, 730046, Gansu, China

    Dan Jia, Jiahui Wang, Junlong Liu, Hehai Li, Aihong Liu, Jinming Wang, Guiquan Guan, Jianxun Luo, Hong Yin & Youquan Li

  2. College of Life Science and Engineering, Lanzhou University of Technology, Lanzhou, 730050, China

    Dan Jia, Ya Wang & Jiahui Wang

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  1. Dan Jia
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  2. Ya Wang
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  3. Jiahui Wang
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  4. Junlong Liu
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  5. Hehai Li
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  6. Aihong Liu
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  7. Jinming Wang
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  8. Guiquan Guan
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  9. Jianxun Luo
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  10. Hong Yin
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  11. Youquan Li
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Contributions

All the authors contributed to this work. DJ analyzed the data and wrote the paper; YW and YL designed the experiments; JW, JL, and HL performed assessment in vitro, JW and GG registered and managed experimental animals, and AL detected samples involved in an experiment in vivo. JL and HY revised the text.

Corresponding author

Correspondence to Youquan Li.

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The authors declare that they have no conflict of interest.

Ethics approval

All of the animal experiments in the study were approved by the Animal Ethics Committee of the Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences. In the present study, all experimental animals used were dealt with according to the Animal Ethic Procedures and Guidelines of the People’s Republic of China (SYXK2010-0001).

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Jia, D., Wang, Y., Wang, J. et al. Lactobacillus animalis pZL8a: a potential probiotic isolated from pig feces for further research. 3 Biotech 11, 132 (2021). https://doi.org/10.1007/s13205-021-02681-3

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