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Identification of Chilli leaf curl virus and associated betasatellite infecting Osteospermum fruticosum in Rajasthan, India

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Abstract

A monopartite begomovirus associated with betasatellite was identified from Osteospermum fruticosum (Cape Daisy) showing severe yellowing vein net symptoms in Rajasthan, India through molecular characterization. The DNA-A shared the highest nucleotide (96.61%) identity to Chilli leaf curl Ahmedabad virus (KM880103), while the betasatellite depicted the highest sequence similarity (99.28%) to Chilli leaf curl betasatellite (JF706231, 99.28%). Based on the sequence identity with other begomoviruses known to date, they were recognized as Chilli leaf curl virus (CDI, MH355641) and Chilli leaf curl betasatellite (CDB1, MH355642), respectively. Phylogenetic analysis showed that DNA-A (CD1) clustered with ChiLCV Goa (KP235539), whereas the betasatellite (CDB1) clustered with ChiLCB Jodhapur (JF70623). Recombination events were observed among the clades of ChiLCV, showing intragenic recombination in Rep (C1) and coat protein (V1/AV1) regions. To our knowledge, this is the first report of ChiLC begomovirus strain affecting O. fruticosum.

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References

  • Briddon RW, Bull SE, Mansoor S et al (2002) Universal primers for the PCR-mediated amplification of DNA β a molecule associated with some monopartite begomoviruses. Mol Biotechnol 20:315–318

    Article  CAS  Google Scholar 

  • Briddon RW, Stanley J (2006) Subviral agents associated with plant single-stranded DNA viruses. Virology 344:198–210

    Article  CAS  Google Scholar 

  • Bull SE, Briddon RW, Markham PG (2003) Universal Primers for the PCR-mediated amplification of DNA 1: a satellite-like molecule associated with begomovirus-DNA beta complexes. Mol Biotechnol 23:83–86

    Article  CAS  Google Scholar 

  • Cui XF, Tao XR, Xie Y, Fauquet CM, Zhou XP (2004) A DNAb associated with Tomato yellow leaf curl China virus is required for symptominduction. J Virol 78:13966–74

    Article  CAS  Google Scholar 

  • Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15

    Google Scholar 

  • Inoue-Nagata AK, Lima MF, Gilbertson RL (2016) A review of geminivirus diseases in vegetables and other crops in Brazil: current status and approaches for management. Horticultura Brasileira 34:8–18

    Article  Google Scholar 

  • Khan AJ, Akhtar S, Al-Zaidi AM et al (2013) Genetic diversity and distribution of a distinct strain of chili leaf curl virus and associated betasatellite infecting tomato and pepper in Oman. Virus Res 177:87–97

    Article  CAS  Google Scholar 

  • Kumar RV, Singh AK, Singh AK, Yadav T, Basu S, Kushwaha N, Chattopadhyay B, Chakraborty S (2015) Complexity of begomovirus and betasatellitepopulation associated with chilli leaf curl disease in India. J General Virol. 96:3143–3158

    Article  CAS  Google Scholar 

  • Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874

    Article  CAS  Google Scholar 

  • Malathi VG, Devi R, Chakraborty S, Biswas KK, Roy A, Venkataravanappa V, Mandal B (2017) Begomoviruses and their satellites occurring in India: distribution, diversity and pathogenesis. In: Mandal B, Rao G, Baranwal V, Jain R (eds) A century of plant virology in India, 1st edn. Springer, Singapore, pp 75–177

    Chapter  Google Scholar 

  • Martin DP, Murrell B, Golden M, Khoosal A, Muhire B (2015) RDP4: Detection and analysis of recombination patterns in virus genomes. Virus Evol 1:vev003

    Article  Google Scholar 

  • Nehra C, Marwal A, Verma RK et al (2019) Papaya yellow leaf curl virus: a newly identified begomovirus infecting Carica papaya L. from the Indian Subcontinent. J Hortic Sci Biotechnol 94:475–480

    Article  Google Scholar 

  • Romay G, Chirinos D, Geraud-Pouey F, Desbiez C (2010) Association of an atypical alphasatellite with a bipartite new world begomovirus. Adv Virol 155:1843–1847

    CAS  Google Scholar 

  • Shahid MS, Illyasb M, Waheed A, Gaur RK (2016) Role of Alphasatellite in Begomoviral Disease Complex. Pak J Sci Ind Res Ser B biol Sci 59(1):52–58

    CAS  Google Scholar 

  • Shahid MS, Raza A, Al-Sadi AM, Briddon RW (2019) Identification of Chilli leaf curl virus associated with tomato leaf curl betasatellite infecting Mentha in Oman. Can J Plant Path 41(2):291–295

    Article  CAS  Google Scholar 

  • Thakur H, Jindal SK, Sharma A, Dhaliwal MS (2018) Chilli leaf curl virus disease: a serious threat for chilli cultivation. J Plant Dis Prot 125:239–249

    Article  Google Scholar 

  • Wu PJ, Zhou XP (2005) Interaction between a nanovirus-like component and the Tobacco curly shoot virus/satellite complex. Acta Biochim Biophys Sin 37(1):25–31

    Article  CAS  Google Scholar 

  • Zhou X (2013) Advances in understanding begomovirus satellites. Annu Rev Phytopathol 51:357–381

    Article  CAS  Google Scholar 

  • Zerbini FM, Briddon RW, Idris A et al (2017) ICTV virus taxonomy profile: Geminiviridae. J Gen Virol 98:131–133

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biosciences, School of Sciences, Mody University of Science and Technology, Lakshmangarh, Sikar, Rajasthan, 332311, India

    Megha Mishra & Rakesh Kumar Verma

  2. Department of Biotechnology, DDU Gorakhpur University, Gorakhpur, Uttar Pradesh, 273009, India

    R. K. Gaur

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  1. Megha Mishra
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  2. Rakesh Kumar Verma
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  3. R. K. Gaur
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Correspondence to R. K. Gaur.

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Mishra, M., Verma, R.K. & Gaur, R.K. Identification of Chilli leaf curl virus and associated betasatellite infecting Osteospermum fruticosum in Rajasthan, India. 3 Biotech 10, 169 (2020). https://doi.org/10.1007/s13205-020-2159-9

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