Abstract
Carotenoids are group of colored terpenoids with antioxidant properties and widespread in nature including in microorganisms. Lactobacillus plantarum subsp. plantarum KCCP11226 was previously isolated from kimchi, while exhibiting the production of 4,4′-diaponeurosporene as a C30 carotenoid. In this study, full genome sequencing of the strain KCCP11226 was performed. Genome analysis revealed that the dehydrosqualene synthase (crtM) and dehydrosqualene desaturase (crtN) genes, which are major genes for biosynthesis of 4,4′-diaponeurosporene, were shown to act as an operon in most L. plantarum strains, but they were uncommon in other Lactobacillus species. In vitro experiments revealed that the production of 4,4′-diaponeurosporene was greatly increased by oxidative stress. In this situation, mRNA expressions of crtN and crtM were also significantly increased. In conclusion, genome analysis of L. plantarum subsp. plantarum KCCP11226 suggested the presence of a well-conserved C30 carotenoid biosynthetic pathway that includes the crtM–crtN operon. The genomic information on L. plantarum subsp. plantarum KCCP11226 could further elucidate the functions of genes involved in isoprenoid biosynthetic pathway, especially in C30 carotenoid biosynthesis.
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Acknowledgements
This work was supported by an Incheon National University Research Grant in 2017.
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M-JS and W-HC designed and coordinated all the experiments; MK performed in vitro experiments into C30 carotenoid production and wrote the manuscript; D-HJ analyzed the genome data; D-HS performed the genome sequencing and sequence assembly. All authors have read and approved the final manuscript.
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Kim, M., Jung, DH., Seo, DH. et al. Genome analysis of Lactobacillus plantarum subsp. plantarum KCCP11226 reveals a well-conserved C30 carotenoid biosynthetic pathway. 3 Biotech 10, 150 (2020). https://doi.org/10.1007/s13205-020-2149-y
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DOI: https://doi.org/10.1007/s13205-020-2149-y