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Neuroblastoma SH-SY5Y cytotoxicity, anti-amyloidogenic activity and cyclooxygenase inhibition of Lasianthus trichophlebus (Rubiaceae)

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Abstract

The anti-amyloidogenic potential and cyclooxygenase anti-inflammatory activity of Lasianthus trichophlebus extracts were evaluated. The MeOH extract (LTM) and chloroform extract (LTC) exhibited significant cytotoxic inhibition against the neuroblastoma SH-SY5Y cell with an IC50 of 17.52 μg/mL and 12.28 μg/mL, respectively. Thioflavin T assay indicated the LTC extract inhibition (70.56% at 50 μg/mL) to be statistically comparable (p < 0.05) to the positive control. Cyclooxygenase inhibition against COX-1 and COX-2 enzymes gave IC50 values for the LTM extract to be 18.20 and 29.60 µg/mL, respectively; while, the LTC extract showed 4.11 and 2.78 µg/mL, respectively. LC–MS of the LTM extract identified 22 putative compounds, which may prove to be pharmacologically relevant. This study has provided potential insights into the utilization of L. trichophlebus to develop safer plant-based agents for anti-inflammatory or neurodegenerative diseases.

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Acknowledgements

The Pascual Pharma Corporation is gratefully acknowledged for the LC–MS analysis and the Philippine DOST-NSC-SEI for the scholarship to M.W.D.L. This research was partly funded by the National Research Foundation of Korea (NRF) Grants awarded by the Korean government (MEST, No. 2017R1A2B4012636).

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Authors and Affiliations

  1. The Graduate School, University of Santo Tomas, Manila, Philippines

    Mario A. Tan, Mark Wilson D. Lagamayo & Grecebio Jonathan D. Alejandro

  2. College of Science, University of Santo Tomas, Manila, Philippines

    Mario A. Tan & Grecebio Jonathan D. Alejandro

  3. Research Center for the Natural and Applied Sciences, University of Santo Tomas, Manila, Philippines

    Mario A. Tan & Grecebio Jonathan D. Alejandro

  4. Department of BionanoTechnology, Bionano Research Institute, Gachon University, Seongnam, South Korea

    Mario A. Tan & Seong Soo A. An

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  1. Mario A. Tan
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  2. Mark Wilson D. Lagamayo
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  3. Grecebio Jonathan D. Alejandro
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  4. Seong Soo A. An
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Contributions

MAT and SSAN conceptualized the study; MWDL performed the experiments; GJDA collected and identified the plant materials; MAT, SSAN, GJDA wrote and revised the manuscript.

Corresponding authors

Correspondence to Mario A. Tan or Seong Soo A. An.

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The authors declare no conflict of interest.

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The in vivo acute oral toxicity in the Online Resource material was approved by the Institutional Animal Care and Use Committee (IACUC) at the Research Center for Natural and Applied Sciences, University of Santo Tomas, and the Philippine Bureau of Animal Industry and Animal Research Permit.

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Tan, M.A., Lagamayo, M.W.D., Alejandro, G.J.D. et al. Neuroblastoma SH-SY5Y cytotoxicity, anti-amyloidogenic activity and cyclooxygenase inhibition of Lasianthus trichophlebus (Rubiaceae). 3 Biotech 10, 152 (2020). https://doi.org/10.1007/s13205-020-2145-2

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