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A potential seedling-stage evaluation method for heat tolerance in Indian mustard (Brassica juncea L. Czern and Coss)

3 Biotech volume 10, Article number: 114 (2020) Cite this article

Abstract

Heat stress is one of the major limitations to crop productivity. In the present study, an efficient method of screening was adopted for identification of heat tolerant Indian Mustard genotypes by applying 4-day cycle of heat stress to seedlings. Thirty-four genotypes were screened based upon lipid peroxidation and survival percentage and classified them into five different classes according to membership function value (MFV) for response against high temperature. The maximum and minimum value of mean MFV were 0.89 (highly heat tolerant, TPM1) and 0.12 (highly heat sensitive, JM2), respectively. The coefficient of determination (R2) between the mean MFV and the heat tolerance index (HTI) of MDA content, survival percentage was 0.914 and 0.808 suggesting that these parameters are reliable traits to evaluate the heat tolerance of Brassica juncea genotypes. The evaluation method was further validated using identified contrasting genotypes and assessment of heat stress associated biochemical parameters. Results showed efficient recovery of tolerant genotype as compared to sensitive genotype. Expression profiling of heat stress-related genes (HSP21 and HSFA7A) showed significant upregulation in the tolerant genotype (TPM1) (9.73- and 4.87-fold, respectively) as compared to the sensitive genotype (JM2) (4.18- and 1.73-fold, respectively) under heat stress condition. The results imply development of an efficient screening method which is useful for evaluation and breeding of thermo-tolerant B. juncea.

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Funding

Research was supported by Bhabha Atomic Research Centre.

Author information

Affiliations

  1. Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Mumbai, 400085, India

    Archana N. Rai & P. Suprasanna

  2. Department of Plant Molecular Biology and Biotechnology, IGKV, Raipur, 492012, India

    Nupur Saini

  3. Division of Germplasm Evaluation, NBPGR, New Delhi, 110012, India

    Rashmi Yadav

Authors
  1. Archana N. Rai
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  2. Nupur Saini
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  3. Rashmi Yadav
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  4. P. Suprasanna
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Contributions

ANR conceived and designed the experiments; RY provided true to type genotype/ germplasm; ANR and NS conducted the experiments; ANR analysed the data; ANR and PS wrote the paper. All authors have read and approved the final manuscript.

Corresponding author

Correspondence to Archana N. Rai.

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Rai, A.N., Saini, N., Yadav, R. et al. A potential seedling-stage evaluation method for heat tolerance in Indian mustard (Brassica juncea L. Czern and Coss). 3 Biotech 10, 114 (2020). https://doi.org/10.1007/s13205-020-2106-9

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  • DOI: https://doi.org/10.1007/s13205-020-2106-9

Keywords

  • Brassica juncea
  • Heat stress
  • Screening of germplasm
  • Heat shock protein 21
  • ROS scavenging