Abstract
The biochemical properties and anti-tumorigenic activity of Cerrena unicolor (CU) and Pycnoporus sanguineus (PS) towards colon cancer cells and the effect of supplementation of the fungal culture medium with cow milk on these activities were examined. CU1-II and PS4-II exhibited anticancer properties through various mechanisms. The extracts at the 200 µg/mL concentration significantly decreased the viability of HT-29 and SW948 cells. They also exhibited pro-apoptotic properties towards the cancer cell lines (HT-29, LS 180, and SW948). Furthermore, culturing the studied fungi on milk-supplemented media may improve the pro-health properties of both milk and mushrooms. The extracts had a higher concentration of proteins, lower levels of free amino acids, and higher content of phenolic compounds than milk. They also exerted a free radical scavenging effect, which may be connected with the high activity of catalase and superoxide dismutase. The tested extracts exhibited anticancer activity: C. unicolor grown on the medium without milk and P. sanguineus grown on the medium with milk. The CU1-II and PS4-II extracts exhibited the strongest anticancer properties; however, PS4-II exerted a milder effect on normal CCD 841 CoTr cells than CU1-II. CU3-II exerted the mildest effect among all extracts on both normal and cancer cells.
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Abbreviations
- aa:
-
Amino acids
- CCD 841 CoTr:
-
Human normal colon epithelial cells
- CU:
-
Cerrena unicolor Extract
- FBS:
-
Fetal bovine serum
- HT-29:
-
Colon adenocarcinoma cells (stage I)
- LPS:
-
Lipopolysaccharide
- LS 180:
-
Colon adenocarcinoma cells (stage II)
- NO:
-
Nitric oxide
- NR:
-
Neutral red
- PI:
-
Propidium iodide
- PS:
-
Pycnoporus sanguineus Extract
- SOD:
-
Superoxide dismutase
- SR101:
-
Sulforhodamine 101
- SW948:
-
Colon adenocarcinoma cells (stage III)
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MP, RP and MF participated in research planning, performed all analyses on cancer and normal cell lines, elaborated the data obtained from experiments on cells in vitro and participated in the process of preparing the manuscript. AZ, MJ, DS, AM and KG participated in research planning, cultivation of the fungi, performed all biochemical analyses, elaborated the data obtained from biochemical experiments and participated in the process of preparing the manuscript.
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Pięt, M., Zając, A., Paduch, R. et al. Chemopreventive activity of bioactive fungal fractions isolated from milk-supplemented cultures of Cerrena unicolor and Pycnoporus sanguineus on colon cancer cells. 3 Biotech 11, 5 (2021). https://doi.org/10.1007/s13205-020-02591-w
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DOI: https://doi.org/10.1007/s13205-020-02591-w