Abstract
We report here the production of an alkaline serine protease by Aspergillus flavus isolated at 5600-m depth from deep-sea sediments of the Central Indian Basin. When grown on defatted groundnut oil meal at 30 °C for 48–72 h, this fungal isolate produced 2000–2500 ACU mL−1 of alkaline protease. The purified protease had activity optima at pH 10.0 and 45 °C. It was a thiol-independent serine protease, identified as an alkaline serine protease ALP1 with a molecular mass of 42.57 kDa. The thermostability and activity of the enzyme increased at 60 °C, in the presence of additives such as sucrose, Tween 20, sorbitol, Ca2+ and glycerol and was not adversely affected by H2O2 indicating its potential as a detergent additive.
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Acknowledgements
The authors wish to thank the Director, CSIR-National Institute of Oceanography for the support and providing all facilities to carry out this study. The second author wishes to thank the Director of the Institute for providing the facilities to carry out the dissertation work as part of the M.Sc. program of Goa University. The authors gratefully acknowledge the funding support received from the project GAP 1248 funded by Department of Biotechnology, Govt. of India, OLP1205 and YSA 1228 funded by Council of Scientific and Industrial Research, Govt. of India and GAP 2128 funded by Ministry of Earth Sciences, Gov. of India. We are also thankful to the anonymous reviewers whose suggestions and constructive comments helped us to improve the manuscript. This manuscript in NIO contribution number 6621.
Funding
The authors gratefully acknowledge the funding support received from the project GAP 1248 funded by Department of Biotechnology, Govt. of India, OLP1205 and YSA 1228 funded by Council of Scientific and Industrial Research, Govt. of India and GAP 2128 (Sampling cruise) funded by Ministry of Earth Sciences, Govt. of India.
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Accession number: 18S rRNA sequence—MT38081.
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Damare, S., Mishra, A., D’Souza-Ticlo-Diniz, D. et al. A deep-sea hydrogen peroxide-stable alkaline serine protease from Aspergillus flavus. 3 Biotech 10, 528 (2020). https://doi.org/10.1007/s13205-020-02520-x
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DOI: https://doi.org/10.1007/s13205-020-02520-x