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Mycochemical composition and insecticidal bioactivity of Algerian desert truffles extract against two stored-product insects: Sitophilus oryzae (L.) (Coleoptera: Curculionidae) and Rhyzopertha dominica (F.) (Coleoptera: Bostrychidae)

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Abstract

The present study aimed to highlight the insecticidal activity of the Algerian desert truffle Terfezia claveryi Chatin against two post-harvest pests of rice and other cereal products, the rice weevil Sitophilus oryzae (L.), and the lesser grain borer Rhyzopertha dominica (F.), using direct contact application method with the filter paper impregnation technique. Soxhlet apparatus was used for extraction with chloroform as solvent. The major compounds in chloroform extract of T. claveryi were identified using gas chromatography–mass spectrometry (GC–MS). The two-way ANOVA was used for statistical analysis to determine the exposure time and doses with the greatest insecticidal activity. Findings of this study revealed that the major compounds were fatty acids: linoleic acid, methyl ester (14.84%), 11,14-eicosadienoic acid, methyl ester (11.55%), oleic acid, methyl ester (7.1%), and palmitic acid (6.96%). However, chamazulene (0.88%) was found to be minor compound. Our results describe for the first time the presence of chamazulene in desert truffle. The result showed also that the most potent insecticidal activity of chloroform extract of T. claveryi was found towards S. oryzea with LD50 value of 162.11 µg/mL. For concentrations of 250 and 300 µg/mL, this extract was able to eradicate 96.65 ± 1.15% and 100.0 ± 0.0% of S. oryzea after 24 h of exposure. However, it caused only 47.24 ± 1.15% and 50.66 ± 1.15% of mortality of R. dominica, respectively, after 6 days. This work offers promising prospects for the use of desert truffles extracts as a potential insecticidal agent for improving quality and safety of stored foods against damage caused by stored-product pests.

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Acknowledgements

The authors acknowledge the financial support of the Algerian General Directorate of Scientific Research and Technological Development (DGRSDT) and the Algerian Ministry of Higher Education and Scientific research (MESRS). We are thankful also to Dr. Malika Gherram for her precious advice and valuable help.

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Authors and Affiliations

  1. Département de Biotechnologie, Faculté des Sciences de la Nature et de la Vie, Laboratoire de Biologie des Microorganismes et de Biotechnologie (LBMB), Université Oran1, Ahmed Benbella , B.P 1524, 31000, El M’naouer, Oran, Algeria

    Samir Neggaz & Fatima El-Houaria Zitouni-Haouar

  2. Département de Chimie, Faculté des Sciences Exactes et Appliquées, Laboratoire de Chimie Fine (LCF), Université Oran1, Ahmed Benbella, B.P 1524, 31000, El M’naouer, Oran, Algeria

    Mohammed Chenni

  3. Institut de Chimie, Université de Nice-Sophia Antipolis, Parc Valrose, 06108, Nice Cedex 2, France

    Xavier Fernandez

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  1. Samir Neggaz
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  2. Mohammed Chenni
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  3. Fatima El-Houaria Zitouni-Haouar
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  4. Xavier Fernandez
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Contributions

SN designed the study, and searched and organized the literature; SN and MC carried out experiments; SN, MC, FE-HZ-H, and XF analyzed the data, and drafted and revised the paper; all authors read and approved the final version of the manuscript.

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Correspondence to Samir Neggaz.

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Neggaz, S., Chenni, M., Zitouni-Haouar, F.EH. et al. Mycochemical composition and insecticidal bioactivity of Algerian desert truffles extract against two stored-product insects: Sitophilus oryzae (L.) (Coleoptera: Curculionidae) and Rhyzopertha dominica (F.) (Coleoptera: Bostrychidae). 3 Biotech 10, 481 (2020). https://doi.org/10.1007/s13205-020-02472-2

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