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Management of plant vigor and soil health using two agriusable nanocompounds and plant growth promotory rhizobacteria in Fenugreek

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Abstract

Application of nanocompounds and plant growth promoting rhizobacteria (PGPR) plays an important role in improving plant growth and soil health. In the present study, response of two PGPR (PS2-KX650178 and PS10-KX650179) along with nanozeolite and nanochitosan was studied on Fenugreek (Trigonella foenum-graecum), on the basis of physiological and biochemical parameters of soil and plant in pot experiment for 45 days. A significant increase (1.5–2 folds) in plant height, leaf number, leaf area and fresh weight over control was observed in Fenugreek plants when treated with nanocompounds and PGPR. Combined treatment also showed the highest level of total chlorophyll (3.27 mg g−1), sugar (6.14 μg mg−1 dry wt), soluble leaf protein (295.37 mg g−1 fresh weight) and catalase activity (23.84 U g−1 tissue) in Fenugreek plants. GC–MS analysis of plant metabolites revealed the abundance of phenols which are known to improve biotic/abiotic stresses in plants. Activity of Fluorescein Diacetate hydrolase enzyme was 2.5 times higher in the combined treatment of nanozeolite with PS10 than in control. An increase of 11% in alkaline phosphatase activity was observed in the same treatment with respect to control. The results obtained from the pot experiment clearly indicate that nanocompounds along with PGPR improved the growth of plants and soil health which suggest their benefits in agriculture practices to increase crop production.

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Abbreviations

ZnONPs:

Zinc oxide nanoparticles

CEC:

Cation exchange capacity

FDA:

Fluorescein diacetate

TOC:

Total organic carbon

Nch:

Nanochitosan

Nz:

Nanozeolite

SNPs:

Silver nanoparticles

Fe3O4 :

Iron oxide

SWCNTs:

Single-walled carbon nanotubes

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Acknowledgements

The authors gratefully acknowledge facilities provided by Department of Microbiology of G.B.Pant University of Agriculture and Technology, Pantnagar.

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Authors and Affiliations

  1. Department of Microbiology, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, 263145, India

    Swati Kumari, Anita Sharma & Parul Chaudhary

  2. Crop Production Division, Vivekananda Parvatiya Krishi Anusandhan Sansthan, Almora, Uttarakhand, 263601, India

    Priyanka Khati

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  1. Swati Kumari
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SK: research work, participated in all the experiments. AS: provided the laboratory facilities and critical checking of the manuscript. PC: wrote the manuscript and helped in data analysis. PK: isolated and characterized the bacterial culture used in the experiment.

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Correspondence to Parul Chaudhary.

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Kumari, S., Sharma, A., Chaudhary, P. et al. Management of plant vigor and soil health using two agriusable nanocompounds and plant growth promotory rhizobacteria in Fenugreek. 3 Biotech 10, 461 (2020). https://doi.org/10.1007/s13205-020-02448-2

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