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Consortia of entomo-pathogenic fungi and bio-control agents improve the agro-ecological conditions for brinjal cultivation of Assam

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Abstract

A liquid consortial formulation with three compatible biocontrol agents viz., Trichoderma harzianum, Beauveria bassiana and Metarhizium anisopliae was prepared with already standardized additives (Assam Agricultural University (AAU), Jorhat, Assam). Pot and field experiments conducted to test the efficacy of six different IPDM (Integrated Pests and Disease Management) modules with the prepared consortia showed that module-6 (seed treatment + seed bed treatment in nursery + soil application in main field + seedling dip treatment with consortia of biofertilizer, Rhizobium sp., Azotobacter sp. strain 52, Azospirillum sp. strain 71 and Bacillus sp. strain 5 W + spraying of consortia) was the best for the management of three important diseases of brinjal viz., Phomopsis leaf blight and fruit rot, Alternaria leaf spot, and Fusarium wilt. Field experiment repeated in the second year ustilizing six different modules revealed the module 6 as best in reduction of disease incidence and increasing the growth parameters and yield of the crop. Result of pooled analysis of field experiments showed a yield of 570.97q/ha with B:C ratio 3.99. The module was also found effective in improving the nutrient status with significant increase in P and K status of soil as well as microbial biomass carbon in both the years of experimentation (2014–2015 and 2015–2016). The module can be used by the farmers for organic cultivation of brinjal after further confirmation in multi locations of diverse agroecological condition of Assam.

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References

  • Banerjee B, Aggarwal PK, Pathak H, Singh AK, Chaudhary A (2006) Dynamics of organic carbon and microbial biomass in alluvial soil with tillage and amendments in rice-wheat systems. Environ Monitor Assess 119:173–189

    CAS  Google Scholar 

  • Baruah TC, Borthakur HP (1997) A text book of soil analysis. Vikas Publishing House Pvt. Ltd., New Delhi

    Google Scholar 

  • Bhagat S, Pan S (2012) Evaluation of Trichoderma spp against seedling diseases of solanaceous vegetables. J Mycopathol Res 50(1):1–10

    Google Scholar 

  • Bhat HA, Ahmed K, Ahangar RA, Quazi NA, Dar NA, Ganie SA (2013) Status and symptomatology of Alternaria leaf blight (Alternaria alternata) of Gerbera (Gerbera jamisonni) in Kashmir valley. Afr J Agric Res 8(9):819–823

    Google Scholar 

  • Bunkar RN, Mathur K (2001) Integration of biocontrol agents and fungicides for suppression of dry root rot of Capsicum frutescens. J Mycol Plant Pathol 31:33–34

    Google Scholar 

  • Dhakshinamoorthy M, Santhy P, Selvi D, Mathan KK (2000) Sustenance of crop productivity and soil fertility on an inceptisol in a long term fertilizer experiment. In: Proceedings of international conference on managing natural resources for sustainable agricultural production in the 21st Century, vol. 3. New Delhi, pp 1336–1337

  • Doifode VD, Nandkar PB (2014) Influence of biofertilizers on the growth, yield and quality of brinjal crop. Int J Life Sci Special Issue A2:17–20

    Google Scholar 

  • Dutta S, Mishra AK, Dileep Kumar BS (2008) Induction of systemic resistance against fusarial wilt in pigeon pea through interaction of plant growth promoting rhizobacteria and rhizobia. Soil Biol Biochem 40:452–461

    CAS  Google Scholar 

  • Ekinci M, Turan M, Ertan YE, Günes A, Kotan R, Dursun A (2014) Effect of plant growth promoting rhizobacteria on growth, nutrient, organic acid, amino acid and hormone content of cauliflower (Brassica oleracea L. var. botrytis) transplants. Acta Sci Pol Hortorum Cultus 13(6):71–85

    Google Scholar 

  • Ganiger VM, Mathad JC, Madalageri MB, Babalad HB, Hebsur NS, Yenagi NB (2012) Effect of organics on the physico-chemical properties of soil after bell pepper cropping under open field condition. Karnataka J Agric Sci 25(4):479–484

    Google Scholar 

  • Glick BR (2012) Plant growth promoting bacteria: mechanisms and applications. Scientifica. https://doi.org/10.6064/2012/963401

    Article  PubMed  PubMed Central  Google Scholar 

  • Horsefall JG, Barratt RW (1945) An improved grading system for measuring plant diseases. Phytopathology 35:655

    Google Scholar 

  • Jackson ML (1973) Soil chemical analysis. Prentice Hall of India Pvt. Ltd., New Delhi

    Google Scholar 

  • Jenkinson DS, Powlson DS (1976) The effect of biocidal treatments on metabolism in soil. V. A method for measuring soil biomass. Soil Biol Biochem 8:209–213

    CAS  Google Scholar 

  • Kaushik H (2015) Persistence and viability of Metarhizium anisopliae in the tea ecosystemof Assam. M.Sc. (Agri) Thesis, AAU, Jorhat,Assam

  • Kay SL, Stewart A (1994) Evaluation of fungal antagonists for control of onion white rot in soil box trials. Plant Pathol 43:371–377

    Google Scholar 

  • Khan VM, Manohar KS, Kumawat SK, Verma HP (2013) Effect of vermicompost and biofertilizers on yield and soil nutrient status after harvest of cowpea (Vigna unguiculata (L.)). W Agric Sustain Dev 1(1):79–81

    Google Scholar 

  • Kleifeld O, Chet I (1992) Trichoderma harzianum-Interaction with plants and effect on growth response. Plant Soil 144:267–272

    Google Scholar 

  • Kumar S (1998) Epidemiology and management of Phomopsis disease of brinjal. Ph.D. Thesis, Department of Plant Pathology, CSK, Himachal Pradesh Agricultural University, Palampur

  • Latha P, Jeyaraman S, Prabakaran R (2014) Study effects of microbial and chemical fertilizer on yield component in brinjal (Solanum melongena Linn.) C. var CO-2. Int J Curr Microbiol Appl Sci 3(8):817–822

    Google Scholar 

  • Manjusha S (1996) Response of culture with graded doses of nitrogen on growth, yield and quality of brinjal and tomato. M.Sc. (Agri) Thesis (Unpub.), Dr. P.D.K.V. Akola (India)

  • Marschner H (1995) Mineral nutrition of higher plants, 2nd edn. Academic Press, London

    Google Scholar 

  • Mohammad N, Alam Z, Nassereldeen AK, Adebayo OS (2011) Development of compatible fungal mixed culture for composting process of oil palm industrial waste. Afr J Biotechnol 10(81):18657–18665

    CAS  Google Scholar 

  • Nathkumar S, Veeraraghavathatham D (2000) Effect of nutrient management on growth parameters and yield of brinjal (Solanum melongena L.) cv PLR-1. South Indian Hort 48(1–6):31–35

    Google Scholar 

  • Ohwaki Y, Hirata H (1992) Differences in carboxylic acid exudation among P-starved leguminous crops in relation to carboxylic acid contents in plant tissues and phospho-lipid level in roots. Soil Sci Plant Nutr 38:235–243

    CAS  Google Scholar 

  • Osowski J (2003) Effectiveness of some chemical strategy protection of potato against an early blight (Alternaria solani). J Pl Prot Res 43(4):361–367

    CAS  Google Scholar 

  • Patten CL, Glick BR (1996) Bacterial biosynthesis of indole-3-acetic acid. Can J Microbiol 42:207–220

    CAS  PubMed  Google Scholar 

  • Pawar SV, Dey U, Munde VG, Sutar DS, Pal D (2013) Management of seed/soil borne diseases of safflower by chemical and biocontrol agents. Afr J Microbiol Res 7(18):1834–1837

    CAS  Google Scholar 

  • Pieterse CMJ, Van Loon LC (1999) Salicylic acid-independent plant defense pathways. Trends Plant Sci 4:52–58

    CAS  PubMed  Google Scholar 

  • Pietr SJ, Wojtkowiak E, Slusarski C, Stankiewicz M, Lewicka T, Biesiada A, Elad Y, Kohl J, Shitenberg D (2002) The possible systemic induction of resistance in some vegetables by fungicide resistant Trichoderma isolates. In: Proceedings of the 7th working group meeting, Influence of Abiotic and Biotic factors on Biocontrol agents at Pine Bay, Kusadasi, Turkey, 22–25 May 2002. Bulletin-OIL-SROP 25, 10,331

  • Piper CS (1966) Soil and plant analysis. Hans Publishers, Bombay

    Google Scholar 

  • Prabhu M, Veeraraghavathatham D, Srinivasan K (2003) Effect of nitrogen phosphorus on growth and yield of brinjal hybrid COBH-1. South Hort 51(1–6):152–156

    Google Scholar 

  • Rahman A, Ali F, Hossain KMA, Laila L (2011) Screening of different eggplant cultivars against wilt caused by fungi, bacteria and nematodes. J Exp Sci 2(1):6–10

    Google Scholar 

  • Raziq F, Ishtiaq S (2010) Integrated control of Alternaria solani with Trichoderma spp and fungicides under in vitro conditions. Sarhad J Agric 26(4):613–619

    Google Scholar 

  • Saravanan T, Muthusamy M, Marimuthu T (2003) Development of integrated approach to manage the fusarial wilt of banana. Crop Protec 22:1117–1123

    Google Scholar 

  • Shailaja B, Patnaik HP, Mukherjee SK, Mishra BK (2011) Effect of indigenous organics on Epilachna beetle incidence, chlorophyll content and fruit yield in brinjal. J Pl Prot Environ 8(2):24–29

    Google Scholar 

  • Sharma M, Razdan VK (2012) Integrated disease management of Phomopsis leaf blight and fruit rot of brinjal (Solanum melongena L.). J Mycol Res 50(2):167–175

    Google Scholar 

  • Sharma P, Sain SK (2005) Use of biotic agents and abiotic compounds against damping off of cauliflower caused by Pythium aphanidermatum. Indian J Phytopathol 58:665395–665401

    Google Scholar 

  • Sicuia O, Dinu S, Dinu M, Fatu C, Valimareanu MC, Constantinescu F (2014) Pests and diseases management using compatible biocontrol bacteria and entomopathogenic fungal strains. Sci Bull Ser F Biotechnol 28:66–72

    Google Scholar 

  • Subbiah BV, Asija GL (1956) A rapid procedure for the determination of available nitrogen in soils. Curr Sci 25:259–260

    CAS  Google Scholar 

  • Suryaminarsih P, Kusriningrum N, Surtiningshih T (2015) Antagonistic compatibility of Streptomyces griseorubens, Gliocladium virens and Trichoderma harzianum against Fusarium oxysporum cause of tomato wilt disease. IJPSS 5(2):82–89

    Google Scholar 

  • Thakur N, Tripathi A (2015) Biological management of damping off, buck eye rot and Fusarial wilt of tomato (cv. Solan Lalima) under Mid-Hill conditions of Himachal Pradesh. Agric Sci 6:535–544

    Google Scholar 

  • Thakur NSA, Firake DM, Behere GT, Firake PD, Saikia K (2012) Biodiversity of agriculturally important insects in North Eastern Himalaya: an overview. Indian J Hill Fmg 25(2):37–40

    Google Scholar 

  • Thilagavathi R, Saravanakumar D, Ragupathi N, Samiyappan R (2007) A combination of biocontrol agents improves the management of dry root rot (Macrophomina phaseolina) in green gram. Phytopathol Mediterr 46:157–167

    CAS  Google Scholar 

  • Upamanya GK, Dutta P (2018) Development of IPDM module for cultivation of brinjal. PhD Thesis, Assam Agricultural University, Jorhat

  • Walkley A, Black IA (1934) An examination of the different method for determining soil organic matter and a proposed modification of the chromic acid titration method. Soil Sci 37:29–38

    CAS  Google Scholar 

  • Windham MT, Elad Y, Baker R (1986) A mechanism for increased plant growth induced by Trichoderma spp. Phytopathol 76:518–521

    Google Scholar 

  • Wu SC, Cao ZH, Li ZG, Cheung KC, Wong MH (2005) Effects of biofertilizer containing N-fixer, P and K solubilizers and AM fungi on maize growth: a greenhouse trial. Geoderma 125:155–166

    Google Scholar 

  • Yabuuchi E, Kosako Y, Yano I, Hotta H, Nishiuchi Y (1995) Transfer of two Burkholderia and an Alcaligenes species to Ralstonia genus nov.: proposal of Ralstonia pickettii (Ralston, Palleroni and Douderoff 1973) comb. nov., Ralstonia solanacearum (Smith 1896) comb. nov. and Ralstonia eutropha (Davis 1969) comb. nov. Microbiol Immunol 39:897–904

    CAS  PubMed  Google Scholar 

  • Yadav RL, Shukla SK, Suman A, Singh PN (2009) Trichoderma inoculation and trash management effects on soil microbial biomass, soil respiration, nutrient uptake and yield of ratoon sugarcane under subtropical conditions. Biol Fert Soils 45:461–468

    Google Scholar 

  • Zegey ED, Santhanam A, Gorfu D, Tessera M, Kassa B (2011) Biocontrol activity of Trichoderma viride and Pseudomonas fluorescens against Phytophthora infestans under green house conditions. J Agr Tech 7(6):1589–1602

    Google Scholar 

  • Zghair QN, Lal A, Mane M, Simon S (2014) Effect of bioagents and fungicide against early blight disease of tomato (Lycopersicon esculentum L.). Internat J Plant Protec 708(2):330–333

    Google Scholar 

  • Zhang Z, Xin H, Zhang J, Raza W, Xing-Ming Y, Yun-ZeR Q-R, Qi-Wei H (2014) Suppression of Fusarium wilt of banana with application of bio-organic fertilizers. Pedosphere 24(5):613–662

    CAS  Google Scholar 

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Acknowledgements

We acknowledge the help, guidance received from Director of Post Graduate Studies, Director of Research (Agri) and Director of Extension of Education, Assam Agricultural University, Jorhat, Assam during the course of the study. We are also thankful to Dr. D. J. Nath, AAU, Jorhat, Assam for providing the biofertilizer strains.

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Authors and Affiliations

  1. Department of Plant Pathology, SCS College of Agriculture, Assam Agricultural University (AAU), Dhubri, Assam, India

    Gunadhya Kumar Upamanya

  2. College of Agriculture, AAU, Jorhat, Assam, India

    Ashok Bhattacharyya

  3. College of Post Graduate Studies in Agricultural Sciences, Central Agricultural University, Umiam, Meghalaya, India

    Pranab Dutta

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  1. Gunadhya Kumar Upamanya
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Correspondence to Gunadhya Kumar Upamanya or Pranab Dutta.

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Upamanya, G.K., Bhattacharyya, A. & Dutta, P. Consortia of entomo-pathogenic fungi and bio-control agents improve the agro-ecological conditions for brinjal cultivation of Assam. 3 Biotech 10, 450 (2020). https://doi.org/10.1007/s13205-020-02439-3

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