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Novel interactions of cardamom mosaic virus VPg with cardamom histones H3 and H4

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Abstract

The host genome targeting potyviral proteins is sparsely reported. Viral genome-linked protein (VPg) is a multifaceted protein known for its interactions with a suite of host proteins, guides essential viral life cycle processes such as genome replication, translation, genome packing, and antiviral defence. Besides, VPg also plays a crucial role in assisting the transport of nuclear inclusion a protease (NIa protease) into the host nucleus. Apart from that, the role of VPg in the nucleus of the cognate host is not clear. Although NIa protease has been reported for DNase activity, the molecular mechanisms underlying host genome accessibility are not yet understood completely. Here, we employed yeast two hybrid assays to test the cardamom histones H3 and H4 interaction with the VPg and NIa protease of macluravirus cardamom mosaic virus (CdMV). Although CdMV NIa protease has the putative histone-binding ER motif of MYST histone acetyltransferase, it did not interact with host histones H3 and H4. Surprisingly, CdMV VPg displayed strong interaction with histone proteins H3 and H4. Leucine prototrophy and β-galactosidase assays were performed which validated VPg interaction with histones. To the best of our knowledge, this study is the first report for the multipartnered potyvirid protein VPg interaction with host histones H3 and H4.

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Acknowledgments

We thank Prof. Yedidya Gafni, Institute of Plant Sciences, Agricultural Research Organization (ARO), Volcani Centre, Bet Dagan, Israel, for his kind gift of yeast two hybrid vectors and EGY48 yeast strain.

Funding

This work was supported by Science and Engineering Research Board (SERB), Department of Science and Technology (DST), Government of India under Project No. CRG/2018/002106.

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Authors and Affiliations

  1. Department of Plant Sciences, School of Biological Sciences, Madurai Kamaraj University, Madurai, Tamil Nadu, 625021, India

    Sankara Naynar Palani, Ramamoorthy Sankaranarayanan & Jebasingh Tennyson

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  1. Sankara Naynar Palani
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  2. Ramamoorthy Sankaranarayanan
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  3. Jebasingh Tennyson
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Contributions

SNP and JT designed this study. The experiments were performed by SNP and RS. SNP wrote the initial draft, and RS and JT edited the manuscript.

Corresponding author

Correspondence to Jebasingh Tennyson.

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The authors declare that they have no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Palani, S.N., Sankaranarayanan, R. & Tennyson, J. Novel interactions of cardamom mosaic virus VPg with cardamom histones H3 and H4. 3 Biotech 10, 444 (2020). https://doi.org/10.1007/s13205-020-02417-9

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