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Developmental stage-associated microbiota profile of the peach fruit fly, Bactrocera zonata (Diptera: Tephritidae) and their functional prediction using 16S rRNA gene metabarcoding sequencing

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Abstract

The different developmental stage-associated microbiota of the peach fruit fly, Bactrocera zonata (Diptera: Tephritidae), was characterized using 16S rRNA gene (V3–V4 region) metabarcoding on the Illumina HiSeq platform. Taxonomically, at 97% similarity, there were total 16 bacterial phyla, comprising of 24 classes, 55 orders, 90 families and 134 genera. Proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes were the most abundant phyla with Gammaproteobacteria, Alphaproteobacteria, Actinobacteria, Bacteroidia and Bacilli being the most abundant classes. The bacterial genus Enterobacter was dominant in the larval and adult stages and Pseudomonas in the pupal stage. A total of 2645 operational taxonomic units (OTUs) were identified, out of which 151 OTUs (core microbiota) were common among all the developmental stages of B. zonata. The genus Enterobacter, Klebsiella and Pantoea were dominant among the core microbiota. PICURSt analysis predicted that microbiota associated with B. zonata may be involved in membrane transport, carbohydrate metabolism, amino acid metabolism, replication and repair processes as well as in cellular processes and signalling. The microbiota that was shared by all the developmental stages of B. zonata in the present study could be targeted and the foundation for research on microbiota-based management of fruit flies.

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Acknowledgements

The authors gratefully acknowledge the anonymous reviewers and executive editor-in-chief for their valuable comments and suggestion on the earlier version of this paper.

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Authors and Affiliations

  1. University Department of Botany, Ranchi University, Morabadi, Ranchi, Jharkhand, 843008, India

    Naiyar Naaz & Ashok Choudhary

  2. ICAR-RCER, Farming System Research Centre for Hill and Plateau Region, Plandu, Ranchi, Jharkhand, 834010, India

    Naiyar Naaz, Jaipal S. Choudhary & Bikash Das

  3. University Department of Zoology, Ranchi University, Morabadi, Ranchi, Jharkhand, 843008, India

    Abhijit Dutta

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Contributions

NN, JSC and BD designed the study; NN and JSC carried out the experiments; NN and JSC analysed the data; and NN, JSC, AC, AD and BD shared in scoping the study, data interpretation and writing the manuscript. All the authors have read and approved the final manuscript.

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Correspondence to Jaipal S. Choudhary.

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The final Sequence Read Achieves (SRAs) of each developmental stage were deposited in the NCBI SRA database with the SRA Accession Numbers: SRX6875946—SRX6875949 under the Bio-Project PRJNA570100.

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Naaz, N., Choudhary, J.S., Choudhary, A. et al. Developmental stage-associated microbiota profile of the peach fruit fly, Bactrocera zonata (Diptera: Tephritidae) and their functional prediction using 16S rRNA gene metabarcoding sequencing. 3 Biotech 10, 390 (2020). https://doi.org/10.1007/s13205-020-02381-4

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