Anxiety determination by antibody-conjugated nanoparticles on an interdigitated electrode sensor

Wang, Xijian; Gopinath, Subash C. B.; Li, Jiexing

doi:10.1007/s13205-020-02370-7

Original Article
Published: 05 August 2020

Anxiety determination by antibody-conjugated nanoparticles on an interdigitated electrode sensor

3 Biotech volume 10, Article number: 377 (2020) Cite this article

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Abstract

This work focused on the detection of cortisol on an interdigitated electrode sensor surface using an anti-cortisol antibody. To improve immobilization, antibodies were conjugated with silver nanoparticles and attached to the surface of the sensor. Cortisol interacted in a dose-dependent manner on the antibody-immobilized sensor surface, and current changes were observed. Linear regression analysis was performed by a 3σ calculation, and the limit of detection fell into the range of 0.01 and 0.1 ng/mL. The sensitivity of cortisol was calculated to be 0.01 ng/mL and the sensor discriminated against other hormones, namely norepinephrine and progesterone, with higher selectivity for cortisol. This result represented the selective detection of cortisol with high performance, which can help to determine anxiety disorders.

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References

Aparna Sharma AH (2015) Serum BDNF: a potential biomarker for major depressive disorder and antidepressant response prediction. J Depress Anxiety 4:55967667. https://doi.org/10.4172/2167-1044.1000179
Article Google Scholar
Brosel-Oliu S, Galyamin D, Abramova N et al (2017) Impedimetric label-free sensor for specific bacteria endotoxin detection by surface charge registration. Electrochim Acta 243:142–151. https://doi.org/10.1016/j.electacta.2017.05.060
CAS Article Google Scholar
Guo S, Lakshmipriya T, Gopinath SCB et al (2019) Complementation of ELISA and an interdigitated electrode surface in gold nanoparticle functionalization for effective detection of human blood clotting defects. Nanoscale Res Lett 14:222. https://doi.org/10.1186/s11671-019-3058-z
CAS Article PubMed PubMed Central Google Scholar
Haarindraprasad R, Hashim U, Gopinath SCB et al (2015) Low temperature annealed zinc oxide nanostructured thin film-based transducers: characterization for sensing applications. PLoS ONE 10:e0132755. https://doi.org/10.1371/journal.pone.0132755
CAS Article PubMed PubMed Central Google Scholar
Horiguchi Y, Miyachi S, Nagasaki Y (2013) High-performance surface acoustic wave immunosensing system on a PEG/aptamer hybridized surface. Langmuir 29:7369–7376. https://doi.org/10.1021/la304548m
CAS Article PubMed Google Scholar
Khan MS, Dighe K, Wang Z, et al (2017) Ultra-sensitive paper-based biosensor for cortisol sensing in human saliva with electrical impedance analyzer. In: 2017 IEEE healthcare innovations and point of care technologies, HI-POCT 2017. pp 184–187
Kim YH, Lee K, Jung H et al (2017) Direct immune-detection of cortisol by chemiresistor graphene oxide sensor. Biosens Bioelectron 98:473–477. https://doi.org/10.1016/j.bios.2017.07.017
CAS Article PubMed Google Scholar
Kinnamon D, Ghanta R, Lin KC et al (2017) Portable biosensor for monitoring cortisol in low-volume perspired human sweat. Sci Rep 7:13312. https://doi.org/10.1038/s41598-017-13684-7
CAS Article PubMed PubMed Central Google Scholar
Lakshmipriya T, Fujimaki M, Gopinath SCB et al (2013) A high-performance waveguide-mode biosensor for detection of factor IX using PEG-based blocking agents to suppress non-specific binding and improve sensitivity. Analyst 138:2863. https://doi.org/10.1039/c3an00298e
CAS Article PubMed Google Scholar
Lakshmipriya T, Hashim U, Gopinath SCB, Azizah N (2016) Microfluidic-based biosensor: signal enhancement by gold nanoparticle. Microsyst Technol 22:2389–2395. https://doi.org/10.1007/s00542-016-3074-1
CAS Article Google Scholar
Letchumanan I, Gopinath SCB, Md Arshad MK et al (2019a) Gold nano-urchin integrated label-free amperometric aptasensing human blood clotting factor IX: a prognosticative approach for “Royal disease”. Biosens Bioelectron 131:128–135. https://doi.org/10.1016/j.bios.2019.02.006
CAS Article PubMed Google Scholar
Letchumanan I, Md Arshad MK, Balakrishnan SR, Gopinath SCB (2019b) Gold-nanorod enhances dielectric voltammetry detection of c-reactive protein: a predictive strategy for cardiac failure. Biosens Bioelectron 130:40–47. https://doi.org/10.1016/j.bios.2019.01.042
CAS Article PubMed Google Scholar
Lin J, Gopinath SCB, Lakshmipriya T et al (2019) Target DNA detection of human papilloma virus-16 E7 gene by capture-target-reporter sandwich on interdigitated electrode sensor. Int J Biol Macromol 141:564–569. https://doi.org/10.1016/j.ijbiomac.2019.09.012
CAS Article PubMed Google Scholar
Liu X, Hsu SPC, Liu WC et al (2019) Salivary electrochemical cortisol biosensor based on tin disulfide nanoflakes. Nanoscale Res Lett 14:189. https://doi.org/10.1186/s11671-019-3012-0
CAS Article PubMed PubMed Central Google Scholar
Luan Y, Lu A, Chen J et al (2016) A label-free aptamer-based fluorescent assay for cadmium detection. Appl Sci 6:432. https://doi.org/10.3390/app6120432
CAS Article Google Scholar
Lv Q, Wang Y, Su C et al (2019) Human papilloma virus DNA-biomarker analysis for cervical cancer: signal enhancement by gold nanoparticle-coupled tetravalent streptavidin-biotin strategy. Int J Biol Macromol 134:354–360. https://doi.org/10.1016/j.ijbiomac.2019.05.044
CAS Article PubMed Google Scholar
Ramanathan S, Gopinath SCB, Md Arshad MK et al (2019) Assorted micro-scale interdigitated aluminium electrode fabrication for insensitive electrolyte evaluation: zeolite nanoparticle-mediated micro- to nano-scaled electrodes. Appl Phys A 125:548. https://doi.org/10.1007/s00339-019-2833-0
CAS Article Google Scholar
Rashkova M (2010) cortisol in saliva—a marker for increased anxiety in children. J IMAB Annu Proc 16:67–69. https://doi.org/10.5272/jimab.1642010_67
Article Google Scholar
Ren X, Meng X, Chen D et al (2005) Using silver nanoparticle to enhance current response of biosensor. Biosens Bioelectron 21:433–437. https://doi.org/10.1016/j.bios.2004.08.052
CAS Article PubMed Google Scholar
Stevens RC, Soelberg SD, Near S, Furlong CE (2008) Detection of cortisol in saliva with a flow-filtered, portable surface plasmon resonance biosensor system. Anal Chem 80:6747–6751. https://doi.org/10.1021/ac800892h
CAS Article PubMed PubMed Central Google Scholar
Upasham S, Tanak A, Jagannath B, Prasad S (2018) Development of ultra-low volume, multi-bio fluid, cortisol sensing platform. Sci Rep 8:16745. https://doi.org/10.1038/s41598-018-35199-5
CAS Article PubMed PubMed Central Google Scholar
Vreeburg SA, Hoogendijk WJG, DeRijk RH et al (2013) Salivary cortisol levels and the 2-year course of depressive and anxiety disorders. Psychoneuroendocrinology 38:1494–1502. https://doi.org/10.1016/j.psyneuen.2012.12.017
CAS Article PubMed Google Scholar
Wang H, Lakshmipriya T, Chen Y, Gopinath SCB (2019) Squamous cell carcinoma biomarker sensing on a strontium oxide-modified interdigitated electrode surface for the diagnosis of cervical cancer. Biomed Res Int 2019:1–7. https://doi.org/10.1155/2019/2807123
CAS Article Google Scholar
Yang L, Li Y (2005) AFM and impedance spectroscopy characterization of the immobilization of antibodies on indium-tin oxide electrode through self-assembled monolayer of epoxysilane and their capture of Escherichia coli O157:H7. Biosens Bioelectron 20:1407–1416. https://doi.org/10.1016/j.bios.2004.06.024
CAS Article PubMed Google Scholar

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Affiliations

Department of Psychiatry, Southeast Corner of the Intersection of Aerospace Avenue and Baomao Expressway, Xi’an Mental Health Center, Chang’an District, Xi’an, 710061, Shaanxi, China
Xijian Wang & Jiexing Li
School of Bioprocess Engineering, Universiti Malaysia Perlis, 02600, Arau, Malaysia
Subash C. B. Gopinath
Institute of Nano Electronic Engineering, Universiti Malaysia Perlis, 01000, Kangar, Perlis, Malaysia
Subash C. B. Gopinath

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Xijian Wang
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Subash C. B. Gopinath
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Jiexing Li
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The authors are contributed to the preparation of the manuscript and discussion. All authors read and approved the final manuscript.

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Correspondence to Jiexing Li.

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Wang, X., Gopinath, S.C.B. & Li, J. Anxiety determination by antibody-conjugated nanoparticles on an interdigitated electrode sensor. 3 Biotech 10, 377 (2020). https://doi.org/10.1007/s13205-020-02370-7

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Received: 19 April 2020
Accepted: 27 June 2020
Published: 05 August 2020
DOI: https://doi.org/10.1007/s13205-020-02370-7

Keywords

Anxiety
Cortisol
Electrochemical sensor
Nanomaterial
Silver nanoparticle