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Biodegradation of carbendazim by a potent novel Chryseobacterium sp. JAS14 and plant growth promoting Aeromonas caviae JAS15 with subsequent toxicity analysis

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Abstract

In the present study, carbendazim (MBC) degrading bacterial strains were isolated and identified as Chryseobacterium sp. JAS14 and Aeromonas caviae JAS15. Both the strains completely degraded 200 mg l−1 of MBC in the aqueous medium and soil within 4–9 days of incubation. In an aqueous medium, the degradation process was characterized by a rate constant of 53.16 day−1 and 42.60 day−1, following zero order model and DT50 was 1.8 days and 2.34 days for Chryseobacterium sp. JAS14 and A. caviae JAS15, respectively. A Chryseobacterium sp. JAS14 and A. caviae JAS15 inoculated into the soil without the addition of nutrients showed the degradation rate constant of 27.30 day−1 and 23.87 day−1, and DT50 was 3.66 days and 4.18 days, respectively. The metabolites during MBC biodegradation by Chryseobacterium sp. JAS14 and A. caviae JAS15 were identified as 2-aminobenzimidazole, 2-hydroxybenzimidazole, 1, 2 diaminobenzene and catechol. To our knowledge, this is the first study of the detailed biodegradation pathway of MBC by Chryseobacterium sp. JAS14 was proposed. Phytotoxicity and cytogenotoxicity assays showed that the toxicity of the MBC reduced after biodegradation by Chryseobacterium sp. JAS14 and A. caviae JAS15. In addition, A. caviae JAS15 possess important plant growth promoting traits under normal and MBC stress condition. These results suggest the Chryseobacterium sp. JAS14 and A. caviae JAS15 could be used as a bioresource for the reclamation of MBC contaminated soil.

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  1. Microbial Biotechnology Laboratory, School of Biosciences and Technology, Vellore Institute of Technology, Vellore, Tamil Nadu, 632014, India

    Sivagnanam Silambarasan & Jayanthi Abraham

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  1. Sivagnanam Silambarasan
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Correspondence to Jayanthi Abraham.

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Silambarasan, S., Abraham, J. Biodegradation of carbendazim by a potent novel Chryseobacterium sp. JAS14 and plant growth promoting Aeromonas caviae JAS15 with subsequent toxicity analysis. 3 Biotech 10, 326 (2020). https://doi.org/10.1007/s13205-020-02319-w

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