Abstract
The effect of different elicitors [jasmonic acid, salicylic acid] and precursors [calcium pantothenate, cholesterol, sodium nitroprusside] on the stimulation of biomass and secondary metabolite production in suspension cultures of Bacopa monnieri was studied. Induction of primary callus cultures was successfully carried out on the Gamborg’s B5 (B5) medium fortified with 2, 4-D (1.0 mg l−1) using Bacopa monnieri leaves as explants. The friable fine suspension cell culture was raised on parent B5 media without agar. The elicitation using different elicitors and precursors at varying concentrations was carried out over a period of 3, 6, 9, 15 days. Elicitor treated cultures showed marked increase in biomass and bacoside production around 6th–9th day (0.98 GI DW). In the present study, salicylic acid at 1.0 mg l−1 induced a maximum elicitation in bacoside content (6.58 mg g−1 DW). The present study provides favorable evidence on the potential of bacoside production using suspension cultures of B. monnieri. The study results also indicate the beneficial effects of elicitation on metabolite production in in vitro suspension cultures of B. monnieri plant known for its cognitive improving properties.
References
Ahuja A, Tripathi MK, Singh SP (2016) Plant cell cultures—an efficient resource for the production of biologically important metabolites: recent development—a review. Prog Res Int J 11(1):1–8
Hussain MS, Fareed S, Ansari S, Rahman MA, Ahmad IZ, Saeed M (2012) Current approaches toward production of secondary plant metabolites. J Pharm Bioallied Sci 4(1):10–20
Isah T (2019) Stress and defense responses in plant secondary metabolites production. Biol Res 52(1):39. https://doi.org/10.1186/s40659-019-0246-3
Kharde A, Kore S, Khetmalas M (2018) Elicitation of bacoside content using plant growth regulators in cell suspension culture of Bacopa monnieri (L.) Wettst. Plant Tissue Cult Biotech 28(2):191–199
Koul A, Sharma A, Gupta S, Mallubhotla S (2014) Cost effective protocol for micropropagation of Bacopa monnieri using leaf explants. Int J Sci Res 3(4):210–212
Lu X, Tang K, Li P (2016) Plant metabolic engineering strategies for the production of pharmaceutical terpenoids. Front Plant Sci 7:1647. https://doi.org/10.3389/fpls.2016.01647
Ochoa-Villarreal M, Howat S, Hong S et al (2016) Plant cell culture strategies for the production of natural products. BMB Rep 49(3):149–158
Ramirez-Estrada K, Vidal-Limon H, Hidalgo D et al (2016) Elicitation, an effective strategy for the biotechnological production of bioactive high-added value compounds in plant cell factories. Molecules 21(2):182. https://doi.org/10.3390/molecules2102018
Sharma M, Ahuja A, Gupta R, Mallubhotla S (2015) Enhanced bacoside production in shoot cultures of Bacopa monnieri under the influence of abiotic elicitors. Nat Prod Res 29(8):745–749
Sharma M, Koul A, Ahuja A, Mallubhotla S (2019) Suitability of bench scale bioreactor system for shoot biomass production and bacoside biosynthesis from Bacopa monnieri (L.). Eng Life Sci 19(8):584–590
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The authors are thankful for the financial assistance from University Grants Commission, Govt. of India (F. No. 41-546/2012).
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Koul, A., Mallubhotla, S. Elicitation and enhancement of bacoside production using suspension cultures of Bacopa monnieri (L.) Wettst. 3 Biotech 10, 256 (2020). https://doi.org/10.1007/s13205-020-02242-0
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DOI: https://doi.org/10.1007/s13205-020-02242-0