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Lipase production from mutagenic strain of Fusarium Incarnatum KU377454 and its immobilization using Au@Ag core shells nanoparticles for application in waste cooking oil degradation

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Abstract

In the present study, lipase production from mutated strain of Fusarium incarnatum KU377454 was optimized through central composite design (CCD) based response surface methodology (RSM). The maximum lipase production (4.01 IU/mL) was obtained within 4 days of incubation using 0.1% CaCl2 concentration and 8% wheat bran concentration. Further, salting out technique was applied for partial purification of lipase. The partially purified lipase was immobilized using Au@Ag bimetallic nanoshell. The characterization of immobilized lipase was carried out by transmission electron microscopy (TEM), field emission scanning electron microscopy (FE-SEM), Fourier transformed infrared (FTIR), energy dispersive X-ray (EDX), X-ray diffraction (XRD) and thermo gravimetric analysis (TGA). The immobilized lipase could retain its 95% of activity after 15 days of storage at 4 °C. Subsequently, Au@Ag immobilized lipase was used for the degradation of waste cooking oil (WCO), which showed higher WCO degradation (85%) compared to the free lipase mediated waste cooking oil degradation (71%). The immobilized lipase could be reused for five times without any loss of its activity.

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Funding

This work was not supported by any funding authority. It was carried out by our interest.

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Author notes

  1. Ritika Joshi and Rekha Sharma contributed equally.

Authors and Affiliations

  1. Department of Bioscience and Biotechnology, Banasthali Vidyapith, Rajasthan, 304022, India

    Ritika Joshi, Anand Prakash & Arindam Kuila

  2. Department of Chemistry, Banasthali Vidyapith, Rajasthan, 304022, India

    Rekha Sharma

  3. Plant Tissue Culture and Genetic Engineering, National Agri-Food Biotechnology Institute (NABI), Sector-81 (Knowledge City), PO Manauli, S.A.S. Nagar, Mohali, 140306, Punjab, India

    Rupam Bhunia

Authors
  1. Ritika Joshi
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  2. Rekha Sharma
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  4. Anand Prakash
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  5. Arindam Kuila
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Contributions

Ritika Joshi, Rekha Sharma and Anand Prakash performed all the experiments. Rupam Bhunia and Arindam Kuila planned the work and written the manuscript.

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Correspondence to Arindam Kuila.

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Joshi, R., Sharma, R., Bhunia, R. et al. Lipase production from mutagenic strain of Fusarium Incarnatum KU377454 and its immobilization using Au@Ag core shells nanoparticles for application in waste cooking oil degradation. 3 Biotech 9, 411 (2019). https://doi.org/10.1007/s13205-019-1949-4

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