Skip to main content
Log in

l-Asparaginase production in rotating bed reactor from Rhizopus microsporus IBBL-2 using immobilized Ca-alginate beads

  • Original Article
  • Published:
3 Biotech Aims and scope Submit manuscript

Abstract

The present work reports on the production of extracellular l-asparaginase from Rhizopus microsporus IBBL-2 using submerged fermentation (SmF) process free of glutaminase and urease activities. Primary studies done in shake flask showed that the highest l-asparaginase activity of 12.68 U mL−1 was produced at 72 h with optimized fermentation parameters such as pH 6.0, 4 × 106 fungal cells mL−1, and agitation of 180 rpm at 30 °C using one-factor-at-a-time (OFAT). Different substrates, nitrogen sources, temperature, pH, the initial number of cells and metal ions were tested to determine the impact on enzyme production. l-Asparaginase activity of 17.68 U mL−1 was produced after 48 h using immobilized calcium-alginate (Ca-alginate) cells. 4 × 106 cells mL−1 was entrapped in 3% (W/V) of alginate bead of size 2 mm each at a temperature of 30 °C and pH of 6. The process was optimized using L9 (34) Taguchi Orthogonal Array (OA) technique with a regression coefficient (R2) value of 0.9709, F value of 33.34 and p value of 0.0025. Scale-up studies involving 200-mL and 1-L rotating bed reactor (RBR) using immobilized beads were done and the results obtained are 20.21 U mL−1 and 19.13 U mL−1, respectively, the increased activity with immobilization accounts for reduced shear on cells due to increased stability as compared to the free-flowing cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

Download references

Acknowledgements

This work is supported by a research grant from the Department of Science and Technology-Science and Engineering Research Board SERB (SB-EMEQ-048/2014). The authors would like to express their sincere gratitude to Dr. Kruthi Doriya, Vaibhav Lendekar, Venkatesh Mandari, and Ashutosh Nema for their support during the experimentation process.

Author information

Authors and Affiliations

Authors

Contributions

Anup Ashok has done the primary work including experimentation, analysis and writing the paper. Dr. Devarai Santhosh Kumar has supervised the entire process and supported in the experimentation, analyses and correction of the manuscript.

Corresponding author

Correspondence to Santhosh Kumar Devarai.

Ethics declarations

Conflict of interest

On behalf of all authors, the corresponding author states that there is no conflict of interest.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ashok, A., Devarai, S.K. l-Asparaginase production in rotating bed reactor from Rhizopus microsporus IBBL-2 using immobilized Ca-alginate beads. 3 Biotech 9, 349 (2019). https://doi.org/10.1007/s13205-019-1883-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s13205-019-1883-5

Keywords

Navigation