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Construction of a species-specific vector for improved plastid transformation efficiency in Capsicum annuum L.

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Abstract

In the present study, we focused on designing a species-specific chloroplast vector for Capsicum annuum L. and finding out its transformation efficiency compared to a heterologous vector. The plastid transformation vector (CaIA) was designed to target homologous regions trnA and trnI of IR region. A selectable marker gene aadA, whose expression is controlled by psbA promoter and terminator, was cloned between two flanking regions. A heterologous vector pRB95, which targets trnfM and trnG of LSC region along with aadA driven by rrn promoter and psbA terminator, was also used for developing plastid transformation in Capsicum. Cotyledonary explants were bombarded with stabilized biolistic parameters: 900 psi pressure and 9 cm flight distance, and optimized regeneration protocol (0.7 mg/L TDZ + 0.2 mg/L IAA) was used to obtain transplastomic lines on selection medium (300 mg/L spectinomycin). The aadA integration and homoplasmy were confirmed by obtaining 1.2 and 3.7 kb amplicons in CaIA transformants and subsequently verified by Southern blotting, whereas in pRB95 transformants, integration was confirmed by PCR with 1.45 kb and 255 bp amplicons corresponding to aadA integration and flanks, respectively. The transformation efficiencies attained with two plastid vectors were found to be 20%, i.e., 10 transplastomic lines in 50 bombarded plates, with CaIA and 2%, i.e., 1 transplastomic line in 50 bombarded plates, with heterologous pRB95, respectively.

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Acknowledgements

The authors thank Ralph Bock, Germany for providing pRB95 plastid vector used in this study. We greatly acknowledge the financial assistance provided by the Department of Science and Technology-New Delhi (SR/SO/BB011/2010, SERB). KS is thankful to CAS-TWAS and University Grants Commission-PDFSS (No. F./PDFSS-2014-15-ST-TEL-9011). AS is grateful to UGC for BSR-Faculty Fellowship.

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Author notes

  1. Srinivas Kota and Raghuvardhan Lakkam contributed equally.

Authors and Affiliations

  1. Plant Biotechnology Research Unit, Department of Biotechnology, Kakatiya University, Warangal, Telangana, 506009, India

    Srinivas Kota, Raghuvardhan Lakkam, Kirnamayee Kasula, Muralikrishna Narra, V. Rao Allini & Sadanandam Abbagani

  2. Institute of Genetics and Developmental Biology, Beijing, China

    Srinivas Kota, Hao Qiang & Hu Zanmin

  3. Department of Biotechnology, Telangana University, Nizamabad, Telangana, 503322, India

    Kirnamayee Kasula

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  1. Srinivas Kota
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  2. Raghuvardhan Lakkam
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  4. Muralikrishna Narra
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Correspondence to Sadanandam Abbagani.

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Kota, S., Lakkam, R., Kasula, K. et al. Construction of a species-specific vector for improved plastid transformation efficiency in Capsicum annuum L.. 3 Biotech 9, 226 (2019). https://doi.org/10.1007/s13205-019-1747-z

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