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Polymorphism in MC1R, TYR and ASIP genes in different colored feather chickens

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Abstract

Coat color genetics successfully adapted and applied to different animal species, which provides a good demonstration of the concept of comparative genetics. In this study, we sequenced 945 bp fragments of melanocortin 1 receptor (MC1R) gene, 421 bp fragments of exon 1 of tyrosinase (TYR) gene and 266 bp fragments of exon 3 of agouti signaling protein (ASIP) gene for 250 individuals with five plumage color patterns. We detected a total of three SNPs (T398A, T637C, and G920C) in MC1R and built six haplotypes (H1–H6) based on the three SNPs. H5 and H6 haplotypes were mainly concentrated in white and grey chicken. And diplotypes H2H3 occurred in white feather and black-speckle feather with the same frequency. Moreover, a total of three SNPs (C47G, T120C, and T172C) in TYR were found and built six haplotypes (P1–P6) based on the three SNPs. Among them, haplotype P2, P3 and P6 were not occurred in black chicken, the diplotypes P1P6 and P4P6 were only distributed in white, gray and black-speckled feather. We only detected one SNP (T168C) in ASIP gene and found that genotype TT was advantage genotype in the different plumage color groups of chickens. Collectively, our study suggested an association between plumage color and genetic variation of MC1R, TYR and ASIP in chicken.

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Acknowledgements

Research was supported by the China Agriculture Research System (Grant No. CARS-41-G04); the National Science and Technology support planning project (Grant No. 2015BAD03B03); the Province Key Technologies R & D Program of Livestock and Poultry Breeding Programs of Sichuan Province (Grant Nos. 2016NYZ0025 & 2016NYZ0043 & 2016NZ0104).

Author information

Author notes

  1. Chao-wu Yang and Jin-shan Ran contributed equally to this work, and shall share the first author.

  2. Yi-ping Liu and Xiao-song Jiang contributed equally to this work, and shall share the correspondence author.

Authors and Affiliations

  1. Animal Breeding and Genetics Key Laboratory of Sichuan Province, Sichuan Animal Science Academy, Chengdu, 610066, China

    Chao-wu Yang, Chun-lin Yu, Mo-han Qiu, Zeng-rong Zhang, Hua-rui Du, Qing-yun Li, Xia Xiong, Xiao-yan Song, Bo Xia, Chen-ming Hu & Xiao-song Jiang

  2. Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, 611130, China

    Jin-shan Ran & Yi-Ping Liu

  3. Institute of Animal Genetics and Breeding, Sichuan Agricultural University, Chengdu Campus, 211 Huimin Road, Wenjiang, 611130, Sichuan Province, China

    Jin-shan Ran & Yi-Ping Liu

Authors
  1. Chao-wu Yang
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  2. Jin-shan Ran
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  3. Chun-lin Yu
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  4. Mo-han Qiu
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  5. Zeng-rong Zhang
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  6. Hua-rui Du
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  7. Qing-yun Li
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  8. Xia Xiong
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  9. Xiao-yan Song
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  10. Bo Xia
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  11. Chen-ming Hu
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  12. Yi-Ping Liu
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  13. Xiao-song Jiang
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Corresponding authors

Correspondence to Yi-Ping Liu or Xiao-song Jiang.

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All authors have no declared conflict of interest.

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All procedures carried out in this experiment were reviewed and approved by the Institutional Animal Care and Use Committee of Sichuan Agricultural University, China.

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Yang, Cw., Ran, Js., Yu, Cl. et al. Polymorphism in MC1R, TYR and ASIP genes in different colored feather chickens. 3 Biotech 9, 203 (2019). https://doi.org/10.1007/s13205-019-1710-z

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  • DOI: https://doi.org/10.1007/s13205-019-1710-z

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