Abstract
To increase the water solubility of questin and broaden its application in preventing and treating Vibrio diseases in aquaculture, an inclusion complex of questin with 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) was prepared by stirring and coevaporation strategy. The results of thin-layer chromatography and nuclear magnetic resonance spectrum confirmed the inclusion of questin into HP-β-CD. The aqueous solubility of questin in the inclusion complex reached (62.63 ± 1.21) µg/mL, which was 110 times of questin’s original solubility. The preliminary agar diffusion method indicated that questin–HP-β-CD inclusion complex showed enhanced antibacterial activity against Vibrio harveyi compared with free questin. This finding provided a reliable basis for the further application of questin as aquatic antibacterial agent.
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Acknowledgements
This work financially supported by the Priority Academic Program Development of Jiangsu Higher Education Institutions (CXKT20180216), Postgraduate Research & Practice Innovation Program of Jiangsu Province (SJCX18_0942), Natural Science Foundation of Jiangsu Province (BK20151283), Technical Plan Project of Lianyungang (CG1612) and 521 Talented Project of Lianyungang (KKC17001).
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Guo, L., Cao, X., Yang, S. et al. Characterization, solubility and antibacterial activity of inclusion complex of questin with hydroxypropyl-β-cyclodextrin. 3 Biotech 9, 123 (2019). https://doi.org/10.1007/s13205-019-1663-2
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DOI: https://doi.org/10.1007/s13205-019-1663-2