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Characterization, solubility and antibacterial activity of inclusion complex of questin with hydroxypropyl-β-cyclodextrin

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Abstract

To increase the water solubility of questin and broaden its application in preventing and treating Vibrio diseases in aquaculture, an inclusion complex of questin with 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) was prepared by stirring and coevaporation strategy. The results of thin-layer chromatography and nuclear magnetic resonance spectrum confirmed the inclusion of questin into HP-β-CD. The aqueous solubility of questin in the inclusion complex reached (62.63 ± 1.21) µg/mL, which was 110 times of questin’s original solubility. The preliminary agar diffusion method indicated that questin–HP-β-CD inclusion complex showed enhanced antibacterial activity against Vibrio harveyi compared with free questin. This finding provided a reliable basis for the further application of questin as aquatic antibacterial agent.

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Acknowledgements

This work financially supported by the Priority Academic Program Development of Jiangsu Higher Education Institutions (CXKT20180216), Postgraduate Research & Practice Innovation Program of Jiangsu Province (SJCX18_0942), Natural Science Foundation of Jiangsu Province (BK20151283), Technical Plan Project of Lianyungang (CG1612) and 521 Talented Project of Lianyungang (KKC17001).

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Authors and Affiliations

  1. Jiangsu Key Laboratory of Marine Bioresources and Environment, Huaihai Institute of Technology, Lianyungang, 222005, China

    Lei Guo, Xi Cao, Shulin Yang, Xintong Wang, Fei Zhang, Hui Chen & Le Wang

  2. Jiangsu Key Laboratory of Marine Biotechnology, Co-innovation Center of Jiangsu Marine Bio-industry Technology, Huaihai Institute of Technology, Lianyungang, 222005, China

    Lei Guo, Xi Cao, Shulin Yang, Xintong Wang, Ying Wen, Fei Zhang, Hui Chen & Le Wang

  3. Jiangsu Institute of Marine Resources Development, Lianyungang, 222004, China

    Lei Guo

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  1. Lei Guo
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  2. Xi Cao
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  3. Shulin Yang
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  4. Xintong Wang
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  8. Le Wang
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Correspondence to Lei Guo.

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Guo, L., Cao, X., Yang, S. et al. Characterization, solubility and antibacterial activity of inclusion complex of questin with hydroxypropyl-β-cyclodextrin. 3 Biotech 9, 123 (2019). https://doi.org/10.1007/s13205-019-1663-2

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