Abstract
This study evaluates the protective effect of astaxanthin against dichlorvos cytotoxicity in yeast Saccharomyces cerevisiae. Dichlorvos induce a dose-dependent cytotoxicity in yeast cells, which is mediated by oxidative stress. Our experimental results showed pre-treatment with astaxanthin enhances cell viability by 20–30% in yeast cells exposed to dichlorvos. A decrease in DCF fluorescence intensity and lipid peroxidation, increased SOD activity, and glutathione levels in astaxanthin-treated cells indicate that astaxanthin protected the cells against dichlorvos-induced oxidative stress. Reduced chromatin condensation and nuclear fragmentation in astaxanthin pre-treated cells also indicate that astaxanthin rescued the cells from dichlorvos-induced apoptosis. Our overall results suggest that dichlorvos induces oxidative stress-mediated cytotoxicity in yeast cells, and that was rescued by astaxanthin pre-treatment.
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Acknowledgements
This work was supported by grants from DST (EMR/2016/008025), and DBT under DBT-IPLS Program (BT/PR14554/INF/22/125/2010), DST-FIST and UGC-SAP, Govt. of India, for providing lab and instrumentation facility.
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Sudharshan, S.J., Subramaniyan, S., Satheeshan, G. et al. Astaxanthin supplementation reduces dichlorvos-induced cytotoxicity in Saccharomyces cerevisiae. 3 Biotech 9, 88 (2019). https://doi.org/10.1007/s13205-019-1634-7
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DOI: https://doi.org/10.1007/s13205-019-1634-7