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Isolation and characterization of lignin-degrading bacterium Bacillus aryabhattai from pulp and paper mill wastewater and evaluation of its lignin-degrading potential

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Abstract

This study reports the degradation and decolourization capability of a manganese peroxidase enzyme producing bacterium isolated from pulp and paper mill wastewater. The isolate was identified as Bacillus aryabhattai based on biochemical analysis and 16S rRNA gene sequencing. The strain was designated MG966493. This bacterium was able to reduce 67% and 54% colour and lignin, respectively, from the pulp and paper mill wastewater after 144 h of treatment at 32 °C, pH 7.6 and 120 rpm. Further, FT-IR analysis showed that during the lignin degradation process a number of metabolites were produced comprising different functional groups such as carbonyl (C=C), carboxyl (–COOH), alkene (C=C), amines (–NH2), sulphonic (–SO3) and nitro (–NO2). In addition, the SEM analysis showed that the bacterial cells exposed to pulp and paper mill wastewater have rough surfaces with reduced size as compared to the unexposed cells with smooth surfaces. This study concluded that the isolated bacterium B. aryabhattai has significant potential for the bioremediation of pulp and paper mill wastewater and thus, can be applied for their treatment at an industrial scale.

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Acknowledgements

Authors are highly thankful to the University Grant Commission (UGC), Government of India (GOI), New Delhi, India for providing the RGNF Fellowship (F1-17.1/2017-18/RGNF-2017-18-SC-UTT-40299) to Ms. Surabhi Zainith for this work. The financial support received from “Science and Engineering Research Board” (SERB), Department of Science & Technology (DST), Government of India (GOI), New Delhi, India, as “Major Research Project” (Grant no: EEQ/2017/000407) is also dully acknowledged.

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Authors and Affiliations

  1. Laboratory for Bioremediation and Metagenomics Research (LBMR), Department of Microbiology (DM), Babasaheb Bhimrao Ambedkar University (A Central University), Vidya Vihar, Raebareli Road, Lucknow, UP, 226 025, India

    Surabhi Zainith & Ram Naresh Bharagava

  2. Department of Natural Sciences, Faculty of Science and Technology, Middlesex University, The Burroughs, Hendon, London, England, NW4 4BT, UK

    Diane Purchase

  3. Department of Food Science and Biotechnology, Dongguk University-Seoul, Ilsandong-gu, 10326, Goyang-si, Gyeonggi-do, Republic of Korea

    Ganesh Dattatraya Saratale

  4. Institute of Technology and Research, Tiradentes University, Campus Farolandia, Aracaju, Brazil

    Luiz Fernando R. Ferreira

  5. School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huaian, 223003, China

    Muhammad Bilal

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  1. Surabhi Zainith
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  2. Diane Purchase
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  6. Ram Naresh Bharagava
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Correspondence to Ram Naresh Bharagava.

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Zainith, S., Purchase, D., Saratale, G.D. et al. Isolation and characterization of lignin-degrading bacterium Bacillus aryabhattai from pulp and paper mill wastewater and evaluation of its lignin-degrading potential. 3 Biotech 9, 92 (2019). https://doi.org/10.1007/s13205-019-1631-x

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