A solventogenic Clostridium sp. strain CT7 which could utilize glycerol directly to produce high yields of butanol was isolated. In the presence of crude glycerol, strain CT7 synthesized butanol through a unique butanol–ethanol (BE) fermentation pathway in which acetone and 1,3-propanediol (1,3-PDO) were not produced. The genome of strain CT7 which has a G + C content of 30.3% was estimated to be 5.99 Mb and contained 4319 putative Open Reading Frames (ORF). The putative annotated genes, which play major roles in BE production from crude glycerol, included glycerol dehydrogenase gene (gdh), acetoacetyl-CoA transferase gene (ctfA/B), and bifunctional alcohol and aldehyde dehydrogenase gene (adhE). In addition, non-typical BE production is not coupled to 1,3-propanediol formation, which may due to the defect of 1, 3-PDO dehydrogenase gene (dhaT).
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This work was supported by the National Natural Science Foundation of China (21706125, 21727818, 21706124, and 31700092), the Jiangsu Province Natural Science Foundation for Youths (BK20170993, BK20170997), the Key Science and Technology Project of Jiangsu Province (BE2016389), and the Jiangsu Synergetic Innovation Center for Advanced Bio-Manufacture (XTE1840).
Conflict of interest
The authors have declared there was no conflict of interest.
Jiasheng Lu and Tianpeng Chen are contributed equally to this work.
Nucleotide Sequence Accession Numbers: This Whole Genome project has been deposited into GenBank under the accession no. PETE00000000. The version described in this paper is version PETE01000000.
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Lu, J., Chen, T., Jiang, Y. et al. The draft genome sequence of Clostridium sp. strain CT7, an isolate capable of producing butanol but not acetone and 1,3-propanediol from crude glycerol. 3 Biotech 9, 63 (2019). https://doi.org/10.1007/s13205-019-1598-7
- Draft genome sequencing