The healing property of a bioactive wound dressing prepared by the combination of bacterial cellulose (BC) and Zingiber officinale root aqueous extract in rats

Abstract

Gluconacetobacter xylinus was used for production of bacterial cellulose (BC). The obtained BC was washed and floated in the nontoxic dose of the herb aqueous extract that was obtained from the methyl thiazol tetrazolium (MTT) assay. Twenty-four Wistar rats were divided into four separated groups and after inducing the wounds (15 mm in diameter), each group was treated with honey, BC, herb aqueous extract, and the combination of BC–herb aqueous extract. Each day the contraction percentages of the wound sites were measured. On days 3, 7, and 14, two rats from each group were euthanized, the skin samples from the wound regions were achieved and their paraffin blocks were prepared. Finally after trichrome staining, the microscopic examinations were done. MTT assay results indicated that the herb aqueous extract had dose-dependent toxic effects and the nontoxic dose of the extract was prepared and utilized for in vivo assay. Although the macroscopic analysis revealed that the BC and the herb aqueous extract had better activity in the wound contraction percentages than their combination, microscopic analysis indicated that the combination of BC–herb aqueous extract revealed all the characteristics that each material induced in the wound site alone. In conclusion, the speed of the wound healing should not be solely considered and its quality should be considered as well.

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References

  1. Al-Amin ZM, Thomson M et al (2006) Anti-diabetic and hypolipidaemic properties of ginger (Zingiber officinale) in streptozotocin-induced diabetic rats. Br J Nut 96(4):660–666

    CAS  Article  Google Scholar 

  2. Barua CC, Talukdar A et al (2009) “Wound healing activity of methanolic extract of leaves of Alternanthera brasiliana Kuntz using in vivo and in vitro model”. Indian J Exp Biol 47(12):1001–1005

    CAS  PubMed  Google Scholar 

  3. Bhagavathula N, Warner RL et al (2009) A combination of curcumin and ginger extract improves abrasion wound healing in corticosteroid-impaired hairless rat skin. Wound Repair Regen 17(3):360–366

    Article  Google Scholar 

  4. Bishop S, Walker M et al (2003) Importance of moisture balance at the wound-dressing interface. J Wound Care 12(4):125–128

    CAS  Article  Google Scholar 

  5. Esa F, Tasirin SM et al (2014) Overview of bacterial cellulose production and application. Agric Agric Sci Proc 2:113–119

    Google Scholar 

  6. Goel R, Sairam K (2002) Anti-ulcer drugs from indigenous sources with emphasis on Musa sapientum, tamrahbasma, Asparagus racemosus and Zingiber officinale. Indian J Pharmacol 34(2):100–110

    CAS  Google Scholar 

  7. Grzanna R, Lindmark L et al (2005) Ginger—an herbal medicinal product with broad anti-inflammatory actions. J Med Food 8(2):125–132

    CAS  Article  Google Scholar 

  8. Heggers JP, Kucukcelebi A et al (1996) Beneficial effect of Aloe on wound healing in an excisional wound model. J Altern Complementary Med 2(2):271–277

    CAS  Article  Google Scholar 

  9. Ji K, Wang W et al (2016) “Bacterial cellulose synthesis mechanism of facultative anaerobe Enterobacter sp. FY-07”. Sci Rep 6:21863

    CAS  Article  Google Scholar 

  10. Kucińska-Lipka J, Gubanska I et al (2015) Bacterial cellulose in the field of wound healing and regenerative medicine of skin: recent trends and future prospectives. Polym Bull 72(9):2399–2419

    Article  Google Scholar 

  11. Moradian S, Almasi H et al (2018) Development of bacterial cellulose-based active membranes containing herbal extracts for shelf life extension of button mushrooms (Agaricus bisporus). J Food Process Preserv 42(3):e13537

    Article  Google Scholar 

  12. Nayak BS, Pinto Pereira LM (2006) Catharanthus roseus flower extract has wound-healing activity in Sprague Dawley rats. BMC Complement Altern Med 6(41):1472–6882

    Google Scholar 

  13. Ojewole JA (2006) Analgesic, antiinflammatory and hypoglycaemic effects of ethanol extract of Zingiber officinale (Roscoe) rhizomes (Zingiberaceae) in mice and rats. Phytother Res 20(9):764–772

    Article  Google Scholar 

  14. Penna S, Medeiros M et al (2003) Anti-inflammatory effect of the hydralcoholic extract of Zingiber officinale rhizomes on rat paw and skin edema. Phytomedicine 10(5):381–385

    CAS  Article  Google Scholar 

  15. Pilehvar-Soltanahmadi Y, Dadashpour M et al (2018) An overview on application of natural substances incorporated with electrospun nanofibrous scaffolds to development of innovative wound dressings. Mini Rev Med Chem 18(5):414–427

    CAS  Article  Google Scholar 

  16. Pourali P, Yahyaei B (2016) Biological production of silver nanoparticles by soil isolated bacteria and preliminary study of their cytotoxicity and cutaneous wound healing efficiency in rat. J Trace Elem Med Biol 34:22–31

    CAS  Article  Google Scholar 

  17. Pourali P, Yahyaei B (2018) Effect of silver nanoparticles produced by Paenibacillus on rat Cutaneous wound healing. J Mazandaran Univ Med Sci 28(163):107–112

    Google Scholar 

  18. Pourali P, Yahyaei B et al (2014) Impregnation of the bacterial cellulose membrane with biologically produced silver nanoparticles. Curr Microbiol 69(6):785–793

    CAS  Article  Google Scholar 

  19. Pourali P, Razavian N, Zadeh et al (2016) Silver nanoparticles production by two soil isolated bacteria, Bacillus thuringiensis and Enterobacter cloacae, and assessment of their cytotoxicity and wound healing effect in rats. Wound Repair Regen 24(5):860–869

    Article  Google Scholar 

  20. Pourali P, Badiee SH et al (2017) Biosynthesis of gold nanoparticles by two bacterial and fungal strains, Bacillus cereus and Fusarium oxysporum, and assessment and comparison of their nanotoxicity in vitro by direct and indirect assays. Electron J Biotechnol 29:86–93

    CAS  Article  Google Scholar 

  21. Pourali P, Razavianzadeh N et al (2018) Assessment of the cutaneous wound healing efficiency of acidic, neutral and alkaline bacterial cellulose membrane in rat. J Mater Sci Mater Med 29(7):90

    Article  Google Scholar 

  22. Ross P, Mayer R et al (1991) Cellulose biosynthesis and function in bacteria. Microbiol Rev 55(1):35–58

    CAS  PubMed  PubMed Central  Google Scholar 

  23. Sen CK, Gordillo GM et al (2009) Human skin wounds: a major and snowballing threat to public health and the economy. Wound repair Regen 17(6):763–771

    Article  Google Scholar 

  24. Siddhan P, Sakthivel K et al (2016) “Biosynthesis of bacterial cellulose imparting antibacterial property through novel bio-agents”. Res J Biotechnol 11:9

    Google Scholar 

  25. Suwantong O, Ruktanonchai U et al (2008) Electrospun cellulose acetate fiber mats containing asiaticoside or Centella asiatica crude extract and the release characteristics of asiaticoside. Polymer 49(19):4239–4247

    CAS  Article  Google Scholar 

  26. Yahyaei B, Peyvandi N et al (2016) Production, assessment, and impregnation of hyaluronic acid with silver nanoparticles that were produced by Streptococcus pyogenes for tissue engineering applications. Appl Biol Chem 59(2):227–237

    CAS  Article  Google Scholar 

  27. Yahyaei B, Manafi S et al (2018) Production of electrospun polyvinyl alcohol/microbial synthesized silver nanoparticles scaffold for the treatment of fungating wounds. Appl Nanosci 8(3):417–426

    Article  Google Scholar 

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Correspondence to Behrooz Yahyaei.

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Pourali, P., Yahyaei, B. The healing property of a bioactive wound dressing prepared by the combination of bacterial cellulose (BC) and Zingiber officinale root aqueous extract in rats. 3 Biotech 9, 59 (2019). https://doi.org/10.1007/s13205-019-1588-9

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Keywords

  • Bacterial cellulose (BC)
  • Zingiber officinale root
  • Aqueous extract
  • Wound-healing assay