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Phyto anti-biofilm elicitors as potential inhibitors ofHelicobacter pylori

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Abstract

Helicobacterpylori (H. pylori) infection is a global public health concern. Due to its high adaptability in various adverse environments (temperature, pH, adhesion, phenotypic forms), targeting the bacterium is quite challenging. Moreover, due to its high persistence, decreased patience compliance and emerging antibiotic resistance, researchers have been forced to search for novel candidates with lesser or no side effects. Hence, in the current study, phytobioactives have been screened for its anti-biofilm attributes against H.pylori. Gastric biopsy samples have been screened using confirmatory techniques (microbiological, biochemical and molecular) for their virulent and non-virulent biomarkers. Physico-nutritive parameters were standardized. H. pylori biofilms were assessed using microtitre plate assay. Biofilms’ biomass and exopolysaccharide have been evaluated using crystal violet and ruthenium red staining, respectively. Anti-biofilm screening was performed using potent aqueous phytochemicals namely Acorus calamus, Colocasia esculenta and Vitex trifolia. The results indicated the confluent growth of the H. pylori biofilms confirmed through genotyping and grew best at 37 °C for 72 h at a pH of 7.5 on polystyrene plates. Further, among the phytochemicals tested, Acorus calamus exhibited the highestH. pylori anti-biofilm activity via a dose-dependent pattern. The overall observations of the study will pave way for newer approaches to understand and combat bacterial pathogenesis and will contribute towards better health and hygiene.

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Authors and Affiliations

  1. Department of Water and Health Faculty of Life Sciences, JSS Academy of Higher Education & Research, Sri Shivarathreeshwara Nagara, Mysore, Karnataka, 570 015, India

    Ashwini Prasad, Govindaraju Shruthi & Chandan Shivamallu

  2. Department of Biotechnology, Sri Jayachamarajendra College of Engineering, JSS Science and Technology University, Mysore, 570 006, Karnataka, India

    Aishwarya Tripurasundari Devi & M. N. Nagendra Prasad

  3. Department of Biochemistry, School of Basic and Applied Sciences, Dayananda Sagar University, Shavige Malleshwara Hills, Kumaraswamy Layout, Bangalore, Karnataka, 560 078, India

    Farhan Zameer

Authors
  1. Ashwini Prasad
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  2. Aishwarya Tripurasundari Devi
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  3. M. N. Nagendra Prasad
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  4. Farhan Zameer
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  5. Govindaraju Shruthi
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  6. Chandan Shivamallu
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Correspondence to Chandan Shivamallu.

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Prasad, A., Devi, A.T., Prasad, M.N.N. et al. Phyto anti-biofilm elicitors as potential inhibitors ofHelicobacter pylori. 3 Biotech 9, 53 (2019). https://doi.org/10.1007/s13205-019-1582-2

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