Overexpressing wheat low-molecular-weight glutenin subunits in rice (Oryza sativa L. japonica cv. Koami) seeds

Abstract

Genes encoding wheat low-molecular-weight glutenin subunits (LMW-GSs) that confer dough strength and extensibility were previously identified from Korean wheat cultivars. To improve low viscoelasticity of rice (Oryza sativa L.) dough caused by the lack of seed storage proteins comparable to wheat gluten, two genes, LMW03 and LMW28, encoding LMW-GSs are cloned from Korean wheat cultivar Jokyoung. The LMW genes are inserted into binary vectors under the control of the rice endosperm-specific Glu-B1 promoter. Transgenic rice plants expressing LMW03 or LMW28 in their seeds are generated using Agrobacterium-mediated transformation. The expression of recombinant wheat LMW-GS in the transgenic rice seeds was confirmed by SDS-PAGE and immunoblot analysis. Their accumulation in the endosperm and aleurone layers of rice seeds was observed through in situ immuno-hybridization.

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Acknowledgements

This work was supported by grants from the National Institute of Agricultural Science (RDA PJ012458) and the Next-Generation BioGreen 21 Program (RDA PJ013149 and RDA PJ013159), South Korea. This study was also supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2016R1A6A3A11931182) to KC.

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YMJ designed and conducted the experiments; KC conducted the experiments and wrote the manuscript; SHL, JYK, and OH contributed critical reading and revision of the manuscript; JYL contributed scientific advice and corrected the manuscript. All authors have read and approved the manuscript.

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Correspondence to Jong-Yeol Lee.

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Cho, K., Jo, Y., Lim, S. et al. Overexpressing wheat low-molecular-weight glutenin subunits in rice (Oryza sativa L. japonica cv. Koami) seeds. 3 Biotech 9, 49 (2019). https://doi.org/10.1007/s13205-019-1579-x

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Keywords

  • Low-molecular-weight glutenin subunit
  • Transgenic rice
  • Wheat
  • Seed storage protein