Isolation and characterization of an estrogen-degrading Pseudomonas putida strain SJTE-1

Abstract

In this report, Pseudomonas putida SJTE1 isolated from an enrichment culture of sludge was confirmed to degrade natural estrogens (17β-estradiol, estrone, estriol), estrogenic chemicals (naphthalene and phenanthrene) and testosterone. The strain completely degraded 1 mg/L 17β-estradiol in 24 h and transformed it into estrone; 90% and 75% of 50 mg/L and 100 mg/L 17β-estradiol were utilized in 7 days, respectively. The transformation efficiency of this strain against natural estrogens was much higher than that against other estrogenic chemicals. Organic carbon sources, lipopolysaccharide and surfactants could enhance the degradation efficiency of strain SJTE-1 against 17β-estradiol. The adsorption of 17β-estradiol onto the biomass was the premise for transmembrane and cellular utilization of this chemical. This work has the potential to bioremediate the environmental estrogens.

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Availability of data and material

The data supporting the conclusions of this article are included within the article and additional file. The estrogen-degrading P. putida SJTE-1 has been deposited to CGMCC with No. 6585.

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Funding

This work was supported by the National Science Foundation of China (Grant no. 31370152, 31570099).

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RL designed the experiments and wrote the manuscript. DZ and PW performed the experiments. XW assisted the experiments. All the authors discussed the results and commented on the manuscript.

Corresponding author

Correspondence to Rubing Liang.

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The authors declare that they have no competing interests.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Wang, P., Zheng, D. & Liang, R. Isolation and characterization of an estrogen-degrading Pseudomonas putida strain SJTE-1. 3 Biotech 9, 61 (2019). https://doi.org/10.1007/s13205-018-1537-z

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Keywords

  • Environmental estrogens
  • Biodegradation
  • Pseudomonas putida SJTE-1
  • 17β-Estradiol
  • Estrone