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Characterization of Pleurotus djamor neohaplonts recovered by production of protoplasts and chemical dedikaryotization

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Abstract

Production of hybrid strains is accomplished by mating monosporic isolates or neohaplonts, obtained either by chemical dedikaryotization or by production of protoplast. However, differences in growth rate among recovered neohaplonts have been reported. The presence of phenotypic and genetic changes among the neohaplonts recovered either by chemical dedikaryotization or by production of protoplast, was evaluated by measuring growth and morphology, and by molecular characterization using six ISSR markers to identify polymorphisms. Neohaplonts recovered by both methods presented variation in growth rate depending on their compatibility type and recovery method. Using ISSR markers, 59.2% polymorphism was established. Neohaplonts recovered by both monokaryotization procedures presented differences in growth rate and polymorphism.

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Acknowledgements

Financial support received: IPN-SIP Project: 20170419, 20180961.

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Authors and Affiliations

  1. Laboratorio de Cultivos Celulares de la Sección de Estudios de Posgrado e Investigación, UPIBI, Instituto Politécnico Nacional, Barrio la Laguna s/n Ticomán, CP 07340, Mexico City, México

    Abraham Sánchez Hernández, Juan Diego Valenzuela Cobos, Ramón Villanueva Arce, Yolanda de las Mercedes Gómez y Gomez & Gustavo Valencia del Toro

  2. Laboratorio de Farmacobiología, FES Iztacala, Universidad Nacional Autónoma de México, Av de los Barrios No.1. Los Reyes Iztacala, CP 54090, Tlalnepantla, Mexico State, México

    María Eugenia Garín Aguilar

  3. Centro de Ciencias de la Complejidad, Circuito Mario de la Cueva 20, Insurgentes Cuicuilco Universidad Nacional Autónoma de México, Cd. Universitaria, 04510, Mexico City, México

    Joel Herrera Martínez

  4. Departamento de Alimentos y Biotecnología, Facultad de Química, Universidad Nacional Autónoma de México, Cd. Universitaria, 04510, Mexico City, México

    Hermilo Leal Lara

  5. Medicina Traslacional, ESM, Instituto Politécnico Nacional, Salvador Díaz Mirón esq. Plan de San Luis S/N, Miguel Hidalgo, Casco de Santo Tomas, 11340, Mexico City, México

    Paola Berenice Zarate Segura

Authors
  1. Abraham Sánchez Hernández
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  2. Juan Diego Valenzuela Cobos
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  3. Joel Herrera Martínez
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  4. Ramón Villanueva Arce
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  5. Yolanda de las Mercedes Gómez y Gomez
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  6. Paola Berenice Zarate Segura
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  7. María Eugenia Garín Aguilar
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  8. Hermilo Leal Lara
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  9. Gustavo Valencia del Toro
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Correspondence to Gustavo Valencia del Toro.

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Hernández, A.S., Valenzuela Cobos, J.D., Martínez, J.H. et al. Characterization of Pleurotus djamor neohaplonts recovered by production of protoplasts and chemical dedikaryotization. 3 Biotech 9, 24 (2019). https://doi.org/10.1007/s13205-018-1532-4

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