Complete genome sequence of Bacillus velezensis ZY-1-1 reveals the genetic basis for its hemicellulosic/cellulosic substrate-inducible xylanase and cellulase activities

Zhang, Zhen-yu; Raza, Muhammad Fahim; Zheng, Ziqiang; Zhang, Xuhao; Dong, Xinxin; Zhang, Hongyu

doi:10.1007/s13205-018-1490-x

Genome Reports
Published: 31 October 2018

Complete genome sequence of Bacillus velezensis ZY-1-1 reveals the genetic basis for its hemicellulosic/cellulosic substrate-inducible xylanase and cellulase activities

Zhen-yu Zhang ORCID: orcid.org/0000-0002-6128-5113¹,
Muhammad Fahim Raza¹,
Ziqiang Zheng¹,
Xuhao Zhang¹,
Xinxin Dong¹ &
Hongyu Zhang¹

3 Biotech volume 8, Article number: 465 (2018) Cite this article

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Abstract

Bacillus velezensis ZY-1-1 was isolated from the larval gut of the lignocellulose-rich diet-fed scarab beetle, Holotrichia parallela, and confirmed to possess extremely high xylanase (48153.8 ± 412.1 U/L) and relatively moderate cellulase activity (610.1 ± 8.2 U/L). Notably, these xylanase and cellulase activities were enhanced by xylan (1.4 and 5.8-fold, respectively) and cellulose (1.1 and 3.5-fold, respectively), which indicated the hemicellulosic/cellulosic substrate-inducible lignocellulolytic activities of this strain. The complete genome of B. velezensis ZY-1-1 comprises of 3,899,251 bp in a circular chromosome with a G + C content of 46.6%. Among the predicted 3688 protein-coding genes, 24 genes are involved in the degradation of lignocellulose and other polysaccharides, including 8, 7 and 2 critical genes for the degradation of xylan, cellulose and lignin, respectively. This genome-based analysis will facilitate our understanding of the mechanism underlying the biodegradation of lignocellulose and the biotechnological application of this novel lignocellulolytic bacteria or related enzymes.

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Acknowledgements

This study was supported by National Natural Science Foundation of China (31501634 and 30671404) and National Key R&D Program of China (2017YFD0202000).

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Authors and Affiliations

State Key Laboratory of Agricultural Microbiology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People’s Republic of China
Zhen-yu Zhang, Muhammad Fahim Raza, Ziqiang Zheng, Xuhao Zhang, Xinxin Dong & Hongyu Zhang

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Zhen-yu Zhang
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Muhammad Fahim Raza
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Ziqiang Zheng
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Xuhao Zhang
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Xinxin Dong
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Hongyu Zhang
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Correspondence to Hongyu Zhang.

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Zhang, Zy., Raza, M.F., Zheng, Z. et al. Complete genome sequence of Bacillus velezensis ZY-1-1 reveals the genetic basis for its hemicellulosic/cellulosic substrate-inducible xylanase and cellulase activities. 3 Biotech 8, 465 (2018). https://doi.org/10.1007/s13205-018-1490-x

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Received: 02 June 2018
Accepted: 23 October 2018
Published: 31 October 2018
DOI: https://doi.org/10.1007/s13205-018-1490-x

Keywords

Bacillus velezensis
Complete genome
Xylanase
Cellulase
Substrate induction