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Genome-wide overview of Trichosporon akiyoshidainum HP-2023, new insights into its mechanism of dye discoloration

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Abstract

Trichosporon akiyoshidainum HP-2023 completely discolorised Reactive Black 5 (200 mg/l) in 24 h. Manganese peroxidase and phenoloxidase, but no laccase activities were detected throughout the incubation. Total aromatic amines in media with Reactive Black 5 decreased 83% after 24 h, supporting an oxidative mechanism of azo dye discoloration. To unravel the genetic basis of these activities, the genome of Trichosporon akiyoshidainum HP-2023 was sequenced, assembled and annotated de novo. T. akiyoshidainum HP-2023 genome comprises 30 MB with a G+C content of 60.75% and 9019 gene models. Thirty-three putative carbohydrate-active enzymes with auxiliary activities, probably involved in lignin degradation and dye discoloration, were identified in the annotated genome, including two laccases, four extracellular fungal heme-peroxidases, nineteen hydrogen peroxide-producing enzymes, and four benzoquinone oxidoreductases. This report will facilitate further studies of textile-dye discoloration with this and closely related strains and poses questions about the ligninolytic potential of Trichosporon akiyoshidainum HP-2023 and related species.

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Funding

This research was supported by the Agencia Nacional de Promoción Científica Y Tecnológica (Grants Numbers PICT 1154-2013 and PICT 2884-2014). The genome of T. oleaginosus and their ESTs data were produced by the US Department of Energy Joint Genome Institute http://www.jgi.doe.gov/ in collaboration with the user community.

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Author notes

  1. Natalia María Bulacio Gil and Hipólito Fernando Pajot contributed equally to this job.

Authors and Affiliations

  1. PROIMI-CONICET (Planta Piloto de Procesos Industriales Microbiológicos), Av. Belgrano y Caseros (T4001MVB), Tucuman, Argentina

    Natalia María Bulacio Gil, Hipólito Fernando Pajot, María del Milagro Rosales Soro, Lucía Inés Castellanos de Figueroa & Daniel Kurth

  2. Microbiología Superior, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, Tucumán, Argentina

    Lucía Inés Castellanos de Figueroa

Authors
  1. Natalia María Bulacio Gil
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  2. Hipólito Fernando Pajot
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  3. María del Milagro Rosales Soro
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  4. Lucía Inés Castellanos de Figueroa
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  5. Daniel Kurth
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Contributions

NBG and MRS performed the cultivation, DNA extraction and genome comparison of selected genomes. HFP provided T. akiyoshidainum HP-2023, designed the experiments, and contributed to the writing of the manuscript. LICF supervised the project and reviewed the manuscript, while DGK annotated T. akiyoshidainum HP-2023 genome, provided computational support and contributed to the writing of the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Daniel Kurth.

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The authors declare that they have no conflict of interest in the publication.

Ethical standards

This study focused on the analysis of a genome sequence of the cellulolytic fungus Trichosporon akiyoshidainum HP-2023. This article does not describe any studies involving human participants or animals performed by any of the authors. Our manuscript complies with the Ethical Rules applicable for 3 Biotech.

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Bulacio Gil, N.M., Pajot, H.F., Rosales Soro, M.d.M. et al. Genome-wide overview of Trichosporon akiyoshidainum HP-2023, new insights into its mechanism of dye discoloration. 3 Biotech 8, 440 (2018). https://doi.org/10.1007/s13205-018-1465-y

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  • DOI: https://doi.org/10.1007/s13205-018-1465-y

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