Abstract
The objective of this study was to isolate the lactic acid bacteria from fermented silage sample and analyze their antibacterial activities, probiotic properties, and fermentation potential in silage. Eleven lactic acid bacteria (LAB) were selected based on distinct morphologies and preliminary studies. Cell-free supernatant (CFS) was then prepared from the selected strains for antibacterial analysis. L-30 strain and its CFS showed highest inhibition (> 10 mm) against tested foodborne pathogens as compared to other strains. Hereafter, the strain L-30 was named as KCC-30 and used for further studies. KCC-30 can survive in the harsh conditions of GIT such as low pH ( 2) and bile salt environment (oxgal) than standard L. plantarum KACC-91016 (pH 2: 27.2% vs 20.5%; oxgal: 72.3% vs 57.7%, both p < 0.05). In addition, KCC-30 exhibited strong auto-aggregation (68.3% vs 51.5%) and co-aggregation (33% vs 23.9%) properties. For silage experiment, KCC-30 treatment did not alter the nutrient profiles of silage. At the same time, KCC-30 treatment increased the lactic acid content of silage as compared to untreated silage (5.55 DM% vs 3.11 DM%). An increase of lactic acid content in the silage is due to higher lactic acid bacteria population in KCC-30 treated silage (15.33 × 107 CFU/g vs 7.66 × 107 CFU/g) than untreated silage (p < 0.05). Overall data suggested that KCC-30 exhibited strong probiotic potential and improved the quality of Lolium multiflorum silage by increasing the lactic acid level. Therefore, KCC-30 could be considered as potential strain to improve the fermentation quality of L. multiflorum silage.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Adeniyi BA, Adetoye A, Ayeni FA (2015) Antibacterial activities of lactic acid bacteria isolated from cow faeces against potential enteric pathogens. Afr Health Sci 15(3):888–895. https://doi.org/10.4314/ahs.v15i3.24
Al Kassaa I, Hamze M, Hober D, Chihib NE, Drider D (2014) Identification of vaginal lactobacilli with potential probiotic properties isolated from women in North Lebanon. Microb Ecol 67(3):722–734. https://doi.org/10.1007/s00248-014-0384-7.
Alcantara C, Zuniga M (2012) Proteomic and transcriptomic analysis of the response to bile stress of Lactobacillus casei BL23. Microbiology 158(Pt 5):1206–1218. https://doi.org/10.1099/mic.0.055657-0.
Ali G, Liu Q, Yuan X, Dong Z, Desta ST, Li J, Bai X, Shah AA, Shao T (2017) Characteristics of lactic acid bacteria isolates and their effects on the fermentation quality of acacia (Sophora japonica L.) leaf silage at low temperatures. Grassl Sci 63(3):141–149. https://doi.org/10.1111/grs.12162
Altay F, Karbancıoglu-Güler F, Daskaya-Dikmen C, Heperkan D (2013) A review on traditional Turkish fermented non-alcoholic beverages: microbiota, fermentation process and quality characteristics. Int J Food Microbiol 167(1):44–56. https://doi.org/10.1016/j.ijfoodmicro.2013.06.016
Arasu MV, Jung M-W, Kim DH, Ilavenil S, Jane M, Park HS, Al-Dhabi NA, Jeon BT, Choi KC (2014a) Enhancing nutritional quality of silage by fermentation with Lactobacillus plantarum. Indian J Microbiol 54(4):396–402. https://doi.org/10.1007/s12088-014-0473-9
Arasu MV, Jung MW, Ilavenil S, Kim DH, Park HS, Park JW, Al-Dhabi NA, Choi KC (2014b) Characterization, phylogenetic affiliation and probiotic properties of high cell density Lactobacillus strains recovered from silage. J Sci Food Agric 94(12):2429–2440. https://doi.org/10.1002/jsfa.6573
Arena MP, Silvain A, Normanno G, Grieco F, Drider D, Spano G, Fiocco D (2016) Use of Lactobacillus plantarum strains as a bio-control strategy against food-borne pathogenic microorganisms. Front Microbiol. https://doi.org/10.3389/fmicb.2016.00464
Bajagai YS, Klieve AV, Dart PJ, Bryden WL (2016) Probiotics in animal nutrition—production, impact and regulation. Food Agric Organ U N 179(1):1–108
Cezário AS, Ribeiro KG, Santos SA, Valadares Filho SdC, Pereira OG (2015) Silages of Brachiaria brizantha cv. Marandu harvested at two regrowth ages: microbial inoculant responses in silage fermentation, ruminant digestion and beef cattle performance. Anim Feed Sci Technol 208:33–43. https://doi.org/10.1016/j.anifeedsci.2015.06.025.
Chen X, Zhuang Y, Dong Z, Zhang J (2017) Factors influencing the distribution of lactic acid bacteria on Pennisetum grasses. Grassl Sci 63(3):150–158. https://doi.org/10.1111/grs.12161
Ferreira DdJ, Lana RdP, Zanine AdM, Santos EM, Veloso CM, Ribeiro GA (2013) Silage fermentation and chemical composition of elephant grass inoculated with rumen strains of Streptococcus bovis. Anim Feed Sci Technol 183(1–2):22–28. https://doi.org/10.1016/j.anifeedsci.2013.04.020
Filya I (2003a) The effect of Lactobacillus buchneri and Lactobacillus plantarum on the fermentation, aerobic stability, and ruminal degradability of low dry matter corn and sorghum silages. J Dairy Sci 86(11):3575–3581. https://doi.org/10.3168/jds.S0022-0302(03)73963-0
Filya I (2003b) The effect of Lactobacillus buchneri, with or without homofermentative lactic acid bacteria, on the fermentation, aerobic stability and ruminal degradability of wheat, sorghum and maize silages. J Appl Microbiol 95(5):1080–1086
Gaudana SB, Dhanani AS, Bagchi T (2010) Probiotic attributes of Lactobacillus strains isolated from food and of human origin. Br J Nutr 103(11):1620–1628. https://doi.org/10.1017/s0007114509993643
Giraffa G, Chanishvili N, Widyastuti Y (2010) Importance of lactobacilli in food and feed biotechnology. Res Microbiol 161(6):480–487. https://doi.org/10.1016/j.resmic.2010.03.001
Haghshenas B, Nami Y, Haghshenas M, Abdullah N, Rosli R, Radiah D, Yari Khosroushahi A (2015) Bioactivity characterization of Lactobacillus strains isolated from dairy products. Microbiol Open 4(5):803–813. https://doi.org/10.1002/mbo3.280
Hursting SD, Dunlap SM (2012) Obesity, metabolic dysregulation, and cancer: a growing concern and an inflammatory (and microenvironmental) issue. Ann N Y Acad Sci 1271(1):82–87. https://doi.org/10.1111/j.1749-6632.2012.06737.x
Ilavenil S, Park HS, Vijayakumar M, Arasu MV, Kim DH, Ravikumar S, Choi KC (2015) Probiotic potential of lactobacillus strains with antifungal activity isolated from animal manure. Sci World J 2015:1–10
Ilavenil S, Vijayakumar M, Kim daH, Valan Arasu M, Park HS, Ravikumar S, Choi KC (2016) Assessment of probiotic, antifungal and cholesterol lowering properties of Pediococcus pentosaceus KCC-23 isolated from Italian ryegrass. J Sci Food Agric 96(2):593–601. https://doi.org/10.1002/jsfa.7128
Nejati F, Babaei M, Taghi-Zadeh A (2016) Characterisation of Lactobacillus helveticus strains isolated from home-made dairy products in Iran. Int J Dairy Technol 69(1):89–95. https://doi.org/10.1111/1471-0307.12223
Ng SY, Koon SS, Padam BS, Chye FY (2015) Evaluation of probiotic potential of lactic acid bacteria isolated from traditional Malaysian fermented Bambangan (Mangifera pajang). CyTA J Food 13(4):563–572. https://doi.org/10.1080/19476337.2015.1020342
Park S-Y, Lim S-D (2015) Probiotic characteristics of Lactobacillus plantarum FH185 isolated from human feces. Korean J Food Sci Anim Resour 35(5):615–621. https://doi.org/10.5851/kosfa.2015.35.5.615
Rabelo CHS, Basso FC, Lara EC, Jorge LGO, Härter CJ, Mesquita LG, Silva LFP, Reis RA (2018) Effects of Lactobacillus buchneri as a silage inoculant and as a probiotic on feed intake, apparent digestibility and ruminal fermentation and microbiology in wethers fed low-dry-matter whole-crop maize silage. Grass Forage Sci 73(1):67–77. https://doi.org/10.1111/gfs.12303
Srigopalram S, Park HS, Ilavenil S, Kim DH, Arasu MV, Kuppusamy P, Lee KD, Choi KC (2017) Isolation, in vitro probiotic characterization of Lactobacillus plantarum and its role on italian ryegrass silage quality enhancement. Int J Agric Biol 19:164–170
Sucu E, Kalkan H, Canbolat O, Filya I (2016) Effects of ensiling density on nutritive value of maize and sorghum silages. Rev Bras Zoot 45:596–603
Tabacco E, Piano S, Cavallarin L, Bernardes TF, Borreani G (2009) Clostridia spore formation during aerobic deterioration of maize and sorghum silages as influenced by Lactobacillus buchneri and Lactobacillus plantarum inoculants. J Appl Microbiol 107(5):1632–1641. https://doi.org/10.1111/j.1365-2672.2009.04344.x
Valan Arasu M, Jung MW, Kim DH, Park HS, Ilavenil S, Al-Dhabi NA, Choon Choi K (2015) Identification and phylogenetic characterization of novel Lactobacillus plantarum species and their metabolite profiles in grass silage. Ann Microbiol 65(1):15–25. https://doi.org/10.1007/s13213-014-0830-2
Wang G, Zhao Y, Tian F, Jin X, Chen H, Liu X, Zhang Q, Zhao J, Chen Y, Zhang H, Chen W (2014) Screening of adhesive lactobacilli with antagonistic activity against Campylobacter jejuni. Food Control 44:49–57. https://doi.org/10.1016/j.foodcont.2014.03.042
Wasko A, Polak-Berecka M, Targonski Z (2011) Purification and characterization of pullulanase from Lactococcus lactis. Prep Biochem Biotechnol 41(3):252–261. https://doi.org/10.1080/10826068.2011.575316
Wasko A, Kieliszek M, Targonski Z (2012) Purification and characterization of a proteinase from the probiotic Lactobacillus rhamnosus OXY. Prep Biochem Biotechnol 42(5):476–488. https://doi.org/10.1080/10826068.2012.656869
Yadav R, Shukla P (2017) An overview of advanced technologies for selection of probiotics and their expediency: a review. Crit Rev Food Sci Nutr 57(15):3233–3242. https://doi.org/10.1080/10408398.2015.1108957
Yadav R, Puniya AK, Shukla P (2016a) Probiotic properties of Lactobacillus plantarum RYPR1 from an indigenous fermented beverage Raabadi. Front Microbiol 7:1683. https://doi.org/10.3389/fmicb.2016.01683
Yadav R, Singh PK, Puniya AK, Shukla P (2016b) catalytic interactions and molecular docking of bile salt hydrolase (BSH) from L. plantarum RYPR1 and its prebiotic utilization. Front Microbiol 7:2116. https://doi.org/10.3389/fmicb.2016.02116
Zanine AdM, Bonelli EA, de Souza AL, Ferreira DdJ, Santos EM, Ribeiro MD, Geron LJV, Pinho RMA (2016) Effects of Streptococcus bovis isolated from bovine rumen on the fermentation characteristics and nutritive value of tanzania grass silage. Sci World J 2016:8517698, 8512016. https://doi.org/10.1155/2016/8517698
Zhang Y, Xiang X, Lu Q, Zhang L, Ma F, Wang L (2016) Adhesions of extracellular surface-layer associated proteins in Lactobacillus M5-L and Q8-L. J Dairy Sci 99(2):1011–1018. https://doi.org/10.3168/jds.2015-10020.
Acknowledgements
This work was carried out with the support of Cooperative Research Program for Agriculture Science & Technology Development (Project title: Development of Quality Improvement and Standardization Technique for Low Moisture Storage Forage, Project no. “PJ010916012017”) funded by Rural Development Administration, Republic of Korea. This study was also supported by 2014 Postdoctoral Fellowship Program of National Institute of Animal Science funded by Rural Development Administration, Republic of Korea.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors have no conflicts of interest related to this study to disclose.
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Srisesharam, S., Park, H.S., Soundharrajan, I. et al. Evaluation of probiotic Lactobacillus plantarum against foodborne pathogens and its fermentation potential in improving Lolium multiflorum silage quality. 3 Biotech 8, 443 (2018). https://doi.org/10.1007/s13205-018-1449-y
Received:
Accepted:
Published:
DOI: https://doi.org/10.1007/s13205-018-1449-y