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Identification and characterization of the myeloid differentiation factor 88 gene in yellow catfish

3 Biotech volume 8, Article number: 430 (2018) Cite this article

Abstract

Myeloid differentiation factor 88 (MyD88) is an important adapter protein of the innate immune system, but it has never before been reported in yellow catfish (Pelteobagrus fulvidraco). In this study, we cloned and characterized the yellow catfish MyD88 gene. The gene was 1230 bp in length and contained an 876-bp open reading frame which encodes a polypeptide of 291 amino acid residues. The theoretical molecular mass and isoelectric point of this polypeptide were 33.4341 kDa and 5.17, respectively. Furthermore, bioinformatic and phylogenetic analyses grouped yellow catfish MyD88 with MyD88 of other fish. We found that the deduced amino acid sequence showed that the conserved N-terminal death domain and the C-terminal typical Toll/interleukin-1 receptor domain were very similar to those of other fish. Moreover, reverse transcription PCR showed that yellow catfish MyD88 is ubiquitously expressed in all tissues examined, with highest expression levels observed in the spleen and lowest levels in the intestine. Importantly, MyD88 was shown to be significantly up-regulated in the intestines after 30-day dietary supplement of Clostridium butyricum. Collectively, these results indicate that yellow catfish MyD88 has a conserved structure and is probably an important component of innate immunity in yellow catfish. This study is the first to identify and characterize MyD88 in yellow catfish, thereby providing a reference for further research into the yellow catfish innate immune system.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 31402083) and the Fundamental Research Funds of China West Normal University (No. 17E077).

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  1. Lintian Yu and Long Zhang have contributed equally to this work.

Affiliations

  1. Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, 611130, China

    Lintian Yu, Long Zhang & Yiping Liu

  2. Institute of Quality Standards for Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China

    Hua Yang, Guohong Gui & Yingping Xiao

  3. Institute of Ecology, China West Normal University, Nanchong, 637009, China

    Long Zhang

  4. Guangxi Agricultural Vocational College, Nanning, 530007, Guangxi, China

    Lintian Yu

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  1. Lintian Yu
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  2. Long Zhang
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  3. Hua Yang
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  4. Guohong Gui
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  5. Yiping Liu
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Correspondence to Yiping Liu or Yingping Xiao.

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Yu, L., Zhang, L., Yang, H. et al. Identification and characterization of the myeloid differentiation factor 88 gene in yellow catfish. 3 Biotech 8, 430 (2018). https://doi.org/10.1007/s13205-018-1448-z

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  • DOI: https://doi.org/10.1007/s13205-018-1448-z

Keywords

  • Yellow catfish
  • MyD88
  • TLR
  • Clostridium butyricum
  • Intestine
  • Innate immunity