Abstract
Light is an important factor for plant development and has serious effects on the growth, production and quality of potatoes. However, the physical and molecular mechanisms by which potato plantlets cope with different light qualities are not understood. In this study, the potato “Zhuanxinwu”, which is a germplasm potato resource with a high anthocyanin content, was used for physiological and transcriptome profiling analyses to uncover the different mechanisms that occur in response to blue, red and white light conditions, with the white light condition serving as the control. Multiple growth indexes, protective enzyme activity and metabolite accumulation were measured. The results indicated that white light promoted a shift in biomass allocation away from tubers to leaves to enhance dry leaf matter and reduce tuber fresh/dry weight relative to the effects of blue or red light. The leaf area and anthocyanin content values were greater for plants grown in blue light than those grown in white or red light, suggesting that combinations of different spectra were more conducive to regulating potato growth. A total of 2220 differentially expressed genes (DEGs) were found among the three samples, and the DEGs in the three comparison sets were analyzed. A total of 1180 and 984 DEGs were identified in the red light (Red) and blue light (Blue) conditions compared to the control condition, respectively, and 359 DEGs overlapped between the two comparison sets (Blue_vs_White and Red_vs_White). Interestingly, the 24 most common overlapped DEGs were involved in photosynthesis, respiration, and reactive oxygen species (ROS) scavenging. Of these DEGs, four genes involved in photosynthesis and two genes involved in pigment synthesis were highly expressed, implying that some genes could be implemented to cope with different light spectra by regulating the expression of DEGs involved in the corresponding metabolic pathways. In conclusion, our study characterizes physiological responses of potato to different light qualities and identifies potential pathways and candidate genes involved in these responses, thus providing a basis for further research on artificial light regulation of potato plant growth.
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References
Ahuja I, de Vos RC, Bones AM, Hall RD (2010) Plant molecular stress responses face climate change. Trends Plant Sci 15(12):664–674. https://doi.org/10.1016/j.tplants.2010.08.002
Akula R, Ravishankar GA (2011) Influence of abiotic stress signals on secondary metabolites in plants. Plant Signal Behav 6(11):1720–1731. https://doi.org/10.4161/psb.6.11.17613
Basu S, Giri RK, Benazir I, Kumar S, Rajwanshi R, Dwivedi SK, Kumar G (2017) Comprehensive physiological analyses and reactive oxygen species profiling in drought tolerant rice genotypes under salinity stress. Physiol Mol Biol Plants 23(4):837–850. https://doi.org/10.1007/s12298-017-0477-0
Birch PRJ, Bryan G, Fenton B, Gilroy EM, Hein I, Jones JT, Prashar A, Taylor MA, Torrance L, Toth IK (2012) Crops that feed the world 8: potato: are the trends of increased global production sustainable? Food Secur 4(4):477–508. https://doi.org/10.1007/s12571-012-0220-1
Conesa A, Gotz S, Garcia-Gomez JM, Terol J, Talon M, Robles M (2005) Blast2GO: a universal tool for annotation, visualization and analysis in functional genomics research. Bioinformatics 21(18):3674–3676. https://doi.org/10.1093/bioinformatics/bti610
Cramer GR, Urano K, Delrot S, Pezzotti M, Shinozaki K (2011) Effects of abiotic stress on plants: a systems biology perspective. BMC Plant Biol 11:163. https://doi.org/10.1186/1471-2229-11-163
Dai H, Fu M, Yang X, Chen Q (2016) Ethylene inhibited sprouting of potato tubers by influencing the carbohydrate metabolism pathway. J Food Sci Technol 53(8):3166–3174. https://doi.org/10.1007/s13197-016-2290-0
Dinakar C, Djilianov D, Bartels D (2012) Photosynthesis in desiccation tolerant plants: energy metabolism and antioxidative stress defense. Plant Sci 182:29–41. https://doi.org/10.1016/j.plantsci.2011.01.018
Fan X-X, Xu Z-G, Liu X-Y, Tang C-M, Wang L-W, Han X-l (2013) Effects of light intensity on the growth and leaf development of young tomato plants grown under a combination of red and blue light. Sci Hortic 153:50–55. https://doi.org/10.1016/j.scienta.2013.01.017
Geigenberger P (2003) Regulation of sucrose to starch conversion in growing potato tubers. J Exp Bot 54(382):457–465
Hancock RD, Morris WL, Ducreux LJ, Morris JA, Usman M, Verrall SR, Fuller J, Simpson CG, Zhang R, Hedley PE, Taylor MA (2014) Physiological, biochemical and molecular responses of the potato (Solanum tuberosum L.) plant to moderately elevated temperature. Plant Cell Environ 37(2):439–450. https://doi.org/10.1111/pce.12168
Hideg E, Jansen MA, Strid A (2013) UV-B exposure, ROS, and stress: inseparable companions or loosely linked associates? Trends Plant Sci 18(2):107–115. https://doi.org/10.1016/j.tplants.2012.09.003
Hogewoning SW, Trouwborst G, Maljaars H, Poorter H, van Ieperen W, Harbinson J (2010) Blue light dose–responses of leaf photosynthesis, morphology, and chemical composition of Cucumis sativus grown under different combinations of red and blue light. J Exp Bot 61(11):3107–3117. https://doi.org/10.1093/jxb/erq132
Jamil A, Riaz S, Ashraf M, Foolad MR (2011) Gene expression profiling of plants under salt stress. Crit Rev Plant Sci 30(5):435–458. https://doi.org/10.1080/07352689.2011.605739
Larkindale J, Vierling E (2008) Core genome responses involved in acclimation to high temperature. Plant Physiol 146(2):748–761. https://doi.org/10.1104/pp.107.112060
Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method. Methods 25(4):402–408. https://doi.org/10.1006/meth.2001.1262
Ma X, Wang Y, Liu M, Xu J, Xu Z (2015) Effects of green and red lights on the growth and morphogenesis of potato (Solanum tuberosum L.) plantlets in vitro. Sci Hortic 190:104–109. https://doi.org/10.1016/j.scienta.2015.01.006
Ma H, Cao X, Shi S, Li S, Gao J, Ma Y, Zhao Q, Chen Q (2016) Genome-wide survey and expression analysis of the amino acid transporter superfamily in potato (Solanum tuberosum L.). Plant Physiol Biochem 107:164–177. https://doi.org/10.1016/j.plaphy.2016.06.007
Macedo AF, Leal-Costa MV, Tavares ES, Lage CLS, Esquibel MA (2011) The effect of light quality on leaf production and development of in vitro-cultured plants of Alternanthera brasiliana Kuntze. Environ Exp Bot 70(1):43–50. https://doi.org/10.1016/j.envexpbot.2010.05.012
Matsuda R, Yamano T, Murakami K, Fujiwara K (2016) Effects of spectral distribution and photosynthetic photon flux density for overnight LED light irradiation on tomato seedling growth and leaf injury. Sci Hortic 198:363–369. https://doi.org/10.1016/j.scienta.2015.11.045
McNellis TW, Deng XW (1995) Light control of seedling morphogenetic pattern. Plant Cell 7(11):1749–1761. https://doi.org/10.1105/tpc.7.11.1749
Moradi F, Ismail AM (2007) Responses of photosynthesis, chlorophyll fluorescence and ROS-scavenging systems to salt stress during seedling and reproductive stages in rice. Ann Bot 99(6):1161–1173. https://doi.org/10.1093/aob/mcm052
Mortazavi A, Williams BA, McCue K, Schaeffer L, Wold B (2008) Mapping and quantifying mammalian transcriptomes by RNA-seq. Nat Methods 5(7):621–628. https://doi.org/10.1038/nmeth.1226
Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. 15 (3):473–497. doi:https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Nazarian-Firouzabadi F, Visser RGF (2017) Potato starch synthases: functions and relationships. Biochem Biophys Rep 10:7–16. https://doi.org/10.1016/j.bbrep.2017.02.004
Parks BM (2003) The red side of photomorphogenesis. Plant Physiol 133(4):1437–1444. https://doi.org/10.1104/pp.103.029702
Robinson MD, McCarthy DJ, Smyth GK (2010) edgeR: a Bioconductor package for differential expression analysis of digital gene expression data. Bioinformatics 26(1):139–140. https://doi.org/10.1093/bioinformatics/btp616
Rocha ABO, Honório SL, Messias CL, Otón M, Gómez PA (2015) Effect of UV-C radiation and fluorescent light to control postharvest soft rot in potato seed tubers. Sci Hortic 181:174–181. https://doi.org/10.1016/j.scienta.2014.10.045
Skirycz A, Vandenbroucke K, Clauw P, Maleux K, De Meyer B, Dhondt S, Pucci A, Gonzalez N, Hoeberichts F, Tognetti VB, Galbiati M, Tonelli C, Van Breusegem F, Vuylsteke M, Inze D (2011) Survival and growth of Arabidopsis plants given limited water are not equal. Nat Biotechnol 29(3):212–214. https://doi.org/10.1038/nbt.1800
Terashima I, Hanba YT, Tholen D, Niinemets U (2011) Leaf functional anatomy in relation to photosynthesis. Plant Physiol 155(1):108–116. https://doi.org/10.1104/pp.110.165472
Umezawa T, Fujita M, Fujita Y, Yamaguchi-Shinozaki K, Shinozaki K (2006) Engineering drought tolerance in plants: discovering and tailoring genes to unlock the future. Curr Opin Biotechnol 17(2):113–122. https://doi.org/10.1016/j.copbio.2006.02.002
Van Harsselaar JK, Lorenz J, Senning M, Sonnewald U, Sonnewald S (2017) Genome-wide analysis of starch metabolism genes in potato (Solanum tuberosum L.). BMC Genom 18(1):37. https://doi.org/10.1186/s12864-016-3381-z
Vincent R, Nadeau D (1983) A micromethod for the quantitation of cellular proteins in Percoll with the Coomassie brilliant blue dye-binding assay. Anal Biochem 135(2):355–362
Xie C, Mao X, Huang J, Ding Y, Wu J, Dong S, Kong L, Gao G, Li C-Y, Wei L (2011) KOBAS 2.0: a web server for annotation and identification of enriched pathways and diseases. Nucleic Acids Res 39(suppl_2):W316–W322. https://doi.org/10.1093/nar/gkr483
Xu J, Su X, Lim S, Griffin J, Carey E, Katz B, Tomich J, Smith JS, Wang W (2015) Characterisation and stability of anthocyanins in purple-fleshed sweet potato P40. Food Chem 186:90–96. https://doi.org/10.1016/j.foodchem.2014.08.123
Acknowledgements
The study was funded by Grants from the National Natural Science Foundation of China (11674174), Jiangsu Vocational College of Agriculture and Forestry Research Project (2016kj002), Top-notch Academic Program Project of Jiangsu Higher Education Institutions (PPZY2015B173) and National Key R&D Program of China (2017YFB0403903).
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ZX conceived the study and amended the manuscript. JX, ZY, YW and ZX performed experiments and data analysis. JX wrote the paper. All authors have read and approved the final manuscript.
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Xu, J., Yan, Z., Xu, Z. et al. Transcriptome analysis and physiological responses of the potato plantlets in vitro under red, blue, and white light conditions. 3 Biotech 8, 394 (2018). https://doi.org/10.1007/s13205-018-1410-0
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DOI: https://doi.org/10.1007/s13205-018-1410-0